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. 1975 Jan;29(1):90–93. doi: 10.1128/am.29.1.90-93.1975

Preparation of Protoplasts of Group H Streptococci (Streptococcus sanguis)

G B Calandra 1, K M Nugent 1, R M Cole 1
PMCID: PMC186916  PMID: 803288

Abstract

Stable protoplasts of several strains of group H streptococci (Streptococcus sanguis) can be prepared by use of group C streptococcal phage-associated lysin in the presence of 30% raffinose. Sucrose cannot be substituted for raffinose. Protoplast formation did not require the addition of Mg2+; however, this cation enhanced their stability. Some other strains, also presumptive group H streptococci, were not sensitive to phage-associated lysin.

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Selected References

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