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. 1975 Jan;29(1):102–105. doi: 10.1128/am.29.1.102-105.1975

Recovery of Murine Leukemia Virus from Large Volumes of Freshly Harvested Culture Fluids by Using a Single Density Gradient

P Sottong *, P Hill *, M Feeney *, J Klecker *, K Johnson *, R Harris *, C Bell *, K Stafford *
PMCID: PMC186918  PMID: 162965

Abstract

Concentration and purification of murine leukemia virus for use as complement fixation antigens was accomplished by using a single density gradient in the model RK ultracentrifuge.

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Selected References

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  1. Anderson N. G., Waters D. A., Nunley C. E., Gibson R. F., Schilling R. M., Denny E. C., Cline G. B., Babelay E. F., Perardi T. E. K-series centrifuges. I. Development of the K-II continuous-sample-flow-with-banding centrifuge system for vaccine purification. Anal Biochem. 1969 Dec;32(3):460–494. doi: 10.1016/s0003-2697(69)80014-x. [DOI] [PubMed] [Google Scholar]
  2. Grandgenett D. P., Brackmann K., Green M. Large-scale purification of ribonucleic acid tumor viruses by use of continuous-flow density gradient centrifugation. Appl Microbiol. 1973 Sep;26(3):452–454. doi: 10.1128/am.26.3.452-454.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hartley J. W., Rowe W. P., Capps W. I., Huebner R. J. Isolation of naturally occurring viruses of the murine leukemia virus group in tissue culture. J Virol. 1969 Feb;3(2):126–132. doi: 10.1128/jvi.3.2.126-132.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Monroe J. H., Brandt P. M. Rapid semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy. Appl Microbiol. 1970 Aug;20(2):259–262. doi: 10.1128/am.20.2.259-262.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Parks W. P., Scolnick E. M., Ross J., Todaro G. J., Aaronson S. A. Immunological relationships of reverse transcriptases from ribonucleic acid tumor viruses. J Virol. 1972 Jan;9(1):110–115. doi: 10.1128/jvi.9.1.110-115.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rhim J. S., Creasy B., Huebner R. J. Production of altered cell foci by 3-methylcholanthrene in mouse cells infected with AKR leukemia virus. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2212–2216. doi: 10.1073/pnas.68.9.2212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  9. Smith R. E., Bernstein E. H. Production and purification of large amounts of Rous sarcoma virus. Appl Microbiol. 1973 Mar;25(3):346–353. doi: 10.1128/am.25.3.346-353.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Toplin I., Sottong P. Large-volume purification of tumor viruses by use of zonal centrifuges. Appl Microbiol. 1972 May;23(5):1010–1014. doi: 10.1128/am.23.5.1010-1014.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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