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. 1975 Feb;29(2):170–174. doi: 10.1128/am.29.2.170-174.1975

Polarographic Assay of Hydrogen Peroxide Accumulation in Microbial Cultures

Patrick M Dempsey 1, Joseph O'Leary 1,1, Seamus Condon 1
PMCID: PMC186939  PMID: 803813

Abstract

A method is described for determining low concentrations of hydrogen peroxide by using a polarographic oxygen electrode to measure the oxygen released into solution on addition of catalase. A sample can be assayed directly without prior manipulation in 3 min. The method is capable of assaying hydrogen peroxide concentrations as low as 7 μM. The method has proved extremely useful for the assay of hydrogen peroxide secreted into milk by lactic acid bacteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anders R. F., Hogg D. M., Jago G. R. Formation of hydrogen peroxide by group N streptococci and its effect on their growth and metabolism. Appl Microbiol. 1970 Apr;19(4):608–612. doi: 10.1128/am.19.4.608-612.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
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