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. 1975 Feb;29(2):219–223. doi: 10.1128/am.29.2.219-223.1975

Production and Purification of the Thermophilic Bacteriophage TP-84

Israel Epstein *,1, L Leon Campbell *
PMCID: PMC186947  PMID: 234714

Abstract

A new procedure for production and purification of the thermophilic bacteriophage TP-84 in high yields is described. Cultures of Bacillus stearothermophilus strain 10, enriched with nutrients to obtain heavy growth and to prevent sporulation and maintained at a pH of 6.5, were infected with the phage in a 100-liter fermentor. Addition of magnesium chloride (0.01 M) and a temperature of 58 C were essential for maximal phage production. Phage (5 × 1011 infective particles/ml) was precipitated with polyethylene glycol (molecular weight 6,000) in the presence of sodium chloride and was further purified by cesium chloride density centrifugation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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