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. 1995 Nov;147(5):1186–1192.

Apolipoprotein A1-derived amyloid in human aortic atherosclerotic plaques.

P Westermark 1, G Mucchiano 1, T Marthin 1, K H Johnson 1, K Sletten 1
PMCID: PMC1869500  PMID: 7485381

Abstract

Amyloid deposits in the aortic intima are very common in association with atherosclerosis and aging. In the present study, a major fibril protein purified from amyloid present in human atherosclerotic plaques was shown to be a 69-amino acid N-terminal fragment of apolipoprotein AI. Although senile form of localized apolipoprotein AI-derived amyloidosis has recently been documented in pulmonary vessels of dogs, this is the first example of a localized human amyloid derived from this apolipoprotein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blobel G., Dobberstein B. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J Cell Biol. 1975 Dec;67(3):835–851. doi: 10.1083/jcb.67.3.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brewer H. B., Jr, Ronan R., Meng M., Bishop C. Isolation and characterization of apolipoproteins A-I, A-II, and A-IV. Methods Enzymol. 1986;128:223–246. doi: 10.1016/0076-6879(86)28070-2. [DOI] [PubMed] [Google Scholar]
  3. Cornwell G. G., 3rd, Murdoch W. L., Kyle R. A., Westermark P., Pitkänen P. Frequency and distribution of senile cardiovascular amyloid. A clinicopathologic correlation. Am J Med. 1983 Oct;75(4):618–623. doi: 10.1016/0002-9343(83)90443-6. [DOI] [PubMed] [Google Scholar]
  4. Glenner G. G., Eanes E. D., Bladen H. A., Linke R. P., Termine J. D. Beta-pleated sheet fibrils. A comparison of native amyloid with synthetic protein fibrils. J Histochem Cytochem. 1974 Dec;22(12):1141–1158. doi: 10.1177/22.12.1141. [DOI] [PubMed] [Google Scholar]
  5. Gustavsson A., Engström U., Westermark P. Normal transthyretin and synthetic transthyretin fragments form amyloid-like fibrils in vitro. Biochem Biophys Res Commun. 1991 Mar 29;175(3):1159–1164. doi: 10.1016/0006-291x(91)91687-8. [DOI] [PubMed] [Google Scholar]
  6. Hilbich C., Kisters-Woike B., Reed J., Masters C. L., Beyreuther K. Aggregation and secondary structure of synthetic amyloid beta A4 peptides of Alzheimer's disease. J Mol Biol. 1991 Mar 5;218(1):149–163. doi: 10.1016/0022-2836(91)90881-6. [DOI] [PubMed] [Google Scholar]
  7. Johnson K. H., Sletten K., Hayden D. W., O'Brien T. D., Roertgen K. E., Westermark P. Pulmonary vascular amyloidosis in aged dogs. A new form of spontaneously occurring amyloidosis derived from apolipoprotein AI. Am J Pathol. 1992 Nov;141(5):1013–1019. [PMC free article] [PubMed] [Google Scholar]
  8. Luo C. C., Li W. H., Chan L. Structure and expression of dog apolipoprotein A-I, E, and C-I mRNAs: implications for the evolution and functional constraints of apolipoprotein structure. J Lipid Res. 1989 Nov;30(11):1735–1746. [PubMed] [Google Scholar]
  9. Mucchiano G., Cornwell G. G., 3rd, Westermark P. Senile aortic amyloid. Evidence for two distinct forms of localized deposits. Am J Pathol. 1992 Apr;140(4):871–877. [PMC free article] [PubMed] [Google Scholar]
  10. Nichols W. C., Dwulet F. E., Liepnieks J., Benson M. D. Variant apolipoprotein AI as a major constituent of a human hereditary amyloid. Biochem Biophys Res Commun. 1988 Oct 31;156(2):762–768. doi: 10.1016/s0006-291x(88)80909-4. [DOI] [PubMed] [Google Scholar]
  11. Pitkänen P., Westermark P., Cornwell G. G., 3rd Senile systemic amyloidosis. Am J Pathol. 1984 Dec;117(3):391–399. [PMC free article] [PubMed] [Google Scholar]
  12. Skinner M., Shirahama T., Cohen A. S., Deal C. L. The association of amyloid P-component (AP) with the amyloid fibril: an updated method for amyloid fibril protein isolation. Prep Biochem. 1982;12(5):461–476. doi: 10.1080/10826068208070597. [DOI] [PubMed] [Google Scholar]
  13. Soutar A. K., Hawkins P. N., Vigushin D. M., Tennent G. A., Booth S. E., Hutton T., Nguyen O., Totty N. F., Feest T. G., Hsuan J. J. Apolipoprotein AI mutation Arg-60 causes autosomal dominant amyloidosis. Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7389–7393. doi: 10.1073/pnas.89.16.7389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Vigushin D. M., Gough J., Allan D., Alguacil A., Penner B., Pettigrew N. M., Quinonez G., Bernstein K., Booth S. E., Booth D. R. Familial nephropathic systemic amyloidosis caused by apolipoprotein AI variant Arg26. Q J Med. 1994 Mar;87(3):149–154. [PubMed] [Google Scholar]
  15. Westermark P., Engström U., Johnson K. H., Westermark G. T., Betsholtz C. Islet amyloid polypeptide: pinpointing amino acid residues linked to amyloid fibril formation. Proc Natl Acad Sci U S A. 1990 Jul;87(13):5036–5040. doi: 10.1073/pnas.87.13.5036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Westermark P., Sletten K., Johansson B., Cornwell G. G., 3rd Fibril in senile systemic amyloidosis is derived from normal transthyretin. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2843–2845. doi: 10.1073/pnas.87.7.2843. [DOI] [PMC free article] [PubMed] [Google Scholar]

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