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. 1975 Mar;29(3):309–312. doi: 10.1128/am.29.3.309-312.1975

Use of pH Gradient Plates for Increasing the Acid Tolerance of Salmonellae

C N Huhtanen 1
PMCID: PMC186971  PMID: 234717

Abstract

Several strains of salmonellae survived higher concentrations of lactic acid after streaking on the surface of pH gradient plates. Most strains increased their acid tolerance by about 0.8 to 1.0 pH unit (9- to 10-fold), with Salmonella madelia showing the greatest differential, pH 5.2 in the wild strain and pH 4.2 after conditioning. The increased acid resistance was quickly lost after transferring to normal tryptic soy agar. Tests for survival in a liquid medium at pH values lower than those giving visible growth indicated that these pH values were bactericidal rather than bacteriostatic for both the wild and acid-conditioned strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Goepfert J. M., Hicks R. Effect of volatile fatty acids on Salmonella typhimurium. J Bacteriol. 1969 Feb;97(2):956–958. doi: 10.1128/jb.97.2.956-958.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Levine A. S., Fellers C. R. Action of Acetic Acid on Food Spoilage Microörganisms. J Bacteriol. 1940 May;39(5):499–515. doi: 10.1128/jb.39.5.499-515.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Prost E., Riemann H. Food-borne salmonellosis. Annu Rev Microbiol. 1967;21:495–528. doi: 10.1146/annurev.mi.21.100167.002431. [DOI] [PubMed] [Google Scholar]
  4. STOKES J. L., BAYNE H. G. Growth rates of Salmonella colonies. J Bacteriol. 1957 Aug;74(2):200–206. doi: 10.1128/jb.74.2.200-206.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. SZYBALSKI W., BRYSON V. Genetic studies on microbial cross resistance to toxic agents. I. Cross resistance of Escherichia coli to fifteen antibiotics. J Bacteriol. 1952 Oct;64(4):489–499. doi: 10.1128/jb.64.4.489-499.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Smith J. L., Palumbo S. A. Microbiology of Lebanon bologna. Appl Microbiol. 1973 Oct;26(4):489–496. doi: 10.1128/am.26.4.489-496.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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