Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1995 Jul;147(1):136–144.

Detection of numerical alterations for chromosomes 7 and 12 in benign thyroid lesions by in situ hybridization. Histological implications.

B Criado 1, A Barros 1, R F Suijkerbuijk 1, D O Weghuis 1, R Seruca 1, E Fonseca 1, S Castedo 1
PMCID: PMC1869887  PMID: 7604875

Abstract

Polysomies of chromosomes 7 and 12 have been frequently observed by conventional cytogenetics in a subgroup of thyroid follicular adenomas and in some cases of thyroid goiters. To further study possible cytogenetic similarities between these two types of thyroid lesions, we have used fluorescence in situ hybridization (FISH) to detect polysomies of chromosomes 7 and/or 12 in isolated nuclei from frozen and paraffin-embedded material of goiters and thyroid follicular adenomas and compared results with previous ones obtained by flow cytometry and conventional cytogenetics. With a set of two alpha-satellite DNA probes specific for the centromeric regions of chromosomes 7 and 12, used either separately (single-target fluorescence in situ hybridization) or simultaneously (double-target fluorescence in situ hybridization), we detected polysomies of chromosome 7 in 35.7% of the thyroid follicular adenomas and in 10.7% of the goiters. Polysomies of chromosome 12 were detected in 29.6% of the thyroid follicular adenomas and 6.7% of the goiters. The significantly higher frequency of adenomas with numerical alterations for chromosomes 7 and/or 12 supports the idea of a biological continuum and karyotypic evolution between both lesions. It is also noteworthy that polysomies of chromosomes 7 and/or 12 were observed only in lesions with an exclusive (or predominant) microfollicular histological component, as detected by enzymatic in situ hybridization on frozen sections.

Full text

PDF
136

Images in this article

141Figure 1
on p.141
142Figure 2
on p.142
143Figure 3
on p.143

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antonini P., Lévy N., Caillou B., Vénuat A. M., Schlumberger M., Parmentier C., Bernheim A. Numerical aberrations, including trisomy 22 as the sole anomaly, are recurrent in follicular thyroid adenomas. Genes Chromosomes Cancer. 1993 Sep;8(1):63–66. doi: 10.1002/gcc.2870080111. [DOI] [PubMed] [Google Scholar]
  2. Arnoldus E. P., Dreef E. J., Noordermeer I. A., Verheggen M. M., Thierry R. F., Peters A. C., Cornelisse C. J., Van der Ploeg M., Raap A. K. Feasibility of in situ hybridisation with chromosome specific DNA probes on paraffin wax embedded tissue. J Clin Pathol. 1991 Nov;44(11):900–904. doi: 10.1136/jcp.44.11.900. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bartnitzke S., Herrmann M. E., Lobeck H., Zuschneid W., Neuhaus P., Bullerdiek J. Cytogenetic findings on eight follicular thyroid adenomas including one with a t(10;19). Cancer Genet Cytogenet. 1989 May;39(1):65–68. doi: 10.1016/0165-4608(89)90230-6. [DOI] [PubMed] [Google Scholar]
  4. Dal Cin P., Sneyers W., Aly M. S., Segers A., Ostijn F., Van Damme B., Van Den Berghe H. Involvement of 19q13 in follicular thyroid adenoma. Cancer Genet Cytogenet. 1992 May;60(1):99–101. doi: 10.1016/0165-4608(92)90244-3. [DOI] [PubMed] [Google Scholar]
  5. Gama N. B., Gama R., Thomé J. A., Tajara E. H. Cytogenetic analysis of a multinodular thyroid goiter. Cancer Genet Cytogenet. 1991 Aug;55(1):73–77. doi: 10.1016/0165-4608(91)90237-o. [DOI] [PubMed] [Google Scholar]
  6. Herrmann M. E., Lalley P. A. Significance of trisomy 7 in thyroid tumors. Cancer Genet Cytogenet. 1992 Sep;62(2):144–149. doi: 10.1016/0165-4608(92)90253-5. [DOI] [PubMed] [Google Scholar]
  7. Hicks D. G., LiVolsi V. A., Neidich J. A., Puck J. M., Kant J. A. Clonal analysis of solitary follicular nodules in the thyroid. Am J Pathol. 1990 Sep;137(3):553–562. [PMC free article] [PubMed] [Google Scholar]
  8. Hopman A. H., Moesker O., Smeets A. W., Pauwels R. P., Vooijs G. P., Ramaekers F. C. Numerical chromosome 1, 7, 9, and 11 aberrations in bladder cancer detected by in situ hybridization. Cancer Res. 1991 Jan 15;51(2):644–651. [PubMed] [Google Scholar]
  9. Hostetter A. L., Hrafnkelsson J., Wingren S. O., Enestrom S., Nordenskjöld B. A comparative study of DNA cytometry methods for benign and malignant thyroid tissue. Am J Clin Pathol. 1988 Jun;89(6):760–763. doi: 10.1093/ajcp/89.6.760. [DOI] [PubMed] [Google Scholar]
  10. Joensuu H., Klemi P., Eerola E. DNA aneuploidy in follicular adenomas of the thyroid gland. Am J Pathol. 1986 Sep;124(3):373–376. [PMC free article] [PubMed] [Google Scholar]
  11. Lee W., Han K., Harris C. P., Meisner L. F. Detection of aneuploidy and possible deletion in paraffin-embedded rhabdomyosarcoma cells with FISH. Cancer Genet Cytogenet. 1993 Jul 15;68(2):99–103. doi: 10.1016/0165-4608(93)90004-6. [DOI] [PubMed] [Google Scholar]
  12. Lemoine N. R., Mayall E. S., Wyllie F. S., Williams E. D., Goyns M., Stringer B., Wynford-Thomas D. High frequency of ras oncogene activation in all stages of human thyroid tumorigenesis. Oncogene. 1989 Feb;4(2):159–164. [PubMed] [Google Scholar]
  13. Looijenga L. H., Smit V. T., Wessels J. W., Mollevanger P., Oosterhuis J. W., Cornelisse C. J., Devilee P. Localization and polymorphism of a chromosome 12-specific alpha satellite DNA sequence. Cytogenet Cell Genet. 1990;53(4):216–218. doi: 10.1159/000132934. [DOI] [PubMed] [Google Scholar]
  14. Namba H., Matsuo K., Fagin J. A. Clonal composition of benign and malignant human thyroid tumors. J Clin Invest. 1990 Jul;86(1):120–125. doi: 10.1172/JCI114673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Poddighe P. J., Moesker O., Smeets D., Awwad B. H., Ramaekers F. C., Hopman A. H. Interphase cytogenetics of hematological cancer: comparison of classical karyotyping and in situ hybridization using a panel of eleven chromosome specific DNA probes. Cancer Res. 1991 Apr 1;51(7):1959–1967. [PubMed] [Google Scholar]
  16. Poddighe P. J., Ramaekers F. C., Hopman A. H. Interphase cytogenetics of tumours. J Pathol. 1992 Mar;166(3):215–224. doi: 10.1002/path.1711660303. [DOI] [PubMed] [Google Scholar]
  17. Roque L., Castedo S., Gomes P., Soares P., Clode A., Soares J. Cytogenetic findings in 18 follicular thyroid adenomas. Cancer Genet Cytogenet. 1993 May;67(1):1–6. doi: 10.1016/0165-4608(93)90036-l. [DOI] [PubMed] [Google Scholar]
  18. Roque L., Gomes P., Correia C., Soares P., Soares J., Castedo S. Thyroid nodular hyperplasia: chromosomal studies in 14 cases. Cancer Genet Cytogenet. 1993 Aug;69(1):31–34. doi: 10.1016/0165-4608(93)90108-x. [DOI] [PubMed] [Google Scholar]
  19. Sozzi G., Miozzo M., Cariani T. C., Bongarzone I., Pilotti S., Pierotti M. A., Della Porta G. A t(2;3)(q12-13;p24-25) in follicular thyroid adenomas. Cancer Genet Cytogenet. 1992 Nov;64(1):38–41. doi: 10.1016/0165-4608(92)90320-8. [DOI] [PubMed] [Google Scholar]
  20. Suijkerbuijk R. F., van de Veen A. Y., van Echten J., Buys C. H., de Jong B., Oosterhuis J. W., Warburton D. A., Cassiman J. J., Schonk D., Geurts van Kessel A. Demonstration of the genuine iso-12p character of the standard marker chromosome of testicular germ cell tumors and identification of further chromosome 12 aberrations by competitive in situ hybridization. Am J Hum Genet. 1991 Feb;48(2):269–273. [PMC free article] [PubMed] [Google Scholar]
  21. Teyssier J. R., Liautaud-Roger F., Ferre D., Patey M., Dufer J. Chromosomal changes in thyroid tumors. Relation with DNA content, karyotypic features, and clinical data. Cancer Genet Cytogenet. 1990 Dec;50(2):249–263. doi: 10.1016/0165-4608(90)90184-c. [DOI] [PubMed] [Google Scholar]
  22. Waye J. S., England S. B., Willard H. F. Genomic organization of alpha satellite DNA on human chromosome 7: evidence for two distinct alphoid domains on a single chromosome. Mol Cell Biol. 1987 Jan;7(1):349–356. doi: 10.1128/mcb.7.1.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. de Leeuw B., Suijkerbuijk R. F., Olde Weghuis D., Meloni A. M., Stenman G., Kindblom L. G., Balemans M., van den Berg E., Molenaar W. M., Sandberg A. A. Distinct Xp11.2 breakpoint regions in synovial sarcoma revealed by metaphase and interphase FISH: relationship to histologic subtypes. Cancer Genet Cytogenet. 1994 Apr;73(2):89–94. doi: 10.1016/0165-4608(94)90191-0. [DOI] [PubMed] [Google Scholar]
  24. van den Berg E., Oosterhuis J. W., de Jong B., Buist J., Vos A., Dam A., Vermeij B. Cytogenetics of thyroid follicular adenomas. Cancer Genet Cytogenet. 1990 Feb;44(2):217–222. doi: 10.1016/0165-4608(90)90050-k. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES