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. 1975 Apr;29(4):448–450. doi: 10.1128/am.29.4.448-450.1975

Stability of Bacterial Mutants in Saline

A M Kropinski 1
PMCID: PMC187002  PMID: 804847

Abstract

By storage in 1% NaCl, genetically characterized strains of Escherichia coli, Salmonella typhimurium, and Pseudomonas aeruginosa were stable for more than 1 year at 4 C. This method of preservation was more effective than maintenance of the strains in stab culture.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antheunisse J. Viability of lyophilized microorganisms after storage. Antonie Van Leeuwenhoek. 1973;39(2):243–248. doi: 10.1007/BF02578856. [DOI] [PubMed] [Google Scholar]
  2. CHANCE H. L. SALT--A PRESERVATIVE FOR BACTERIAL CULTURES. J Bacteriol. 1963 Mar;85:719–720. doi: 10.1128/jb.85.3.719-720.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DEVAY J. E., SCHNATHORST W. C. SINGLE-CELL ISOLATION AND PRESERVATION OF BACTERIAL CULTURES. Nature. 1963 Aug 24;199:775–777. doi: 10.1038/199775a0. [DOI] [PubMed] [Google Scholar]
  5. Emmanouilidou-Arseni A., Koumentakou I. Viability of Pseudomonas aeruginosa. J Bacteriol. 1964 May;87(5):1253–1253. doi: 10.1128/jb.87.5.1253-.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HOWARD D. H. The preservation of bacteria by freezing in glycerol broth. J Bacteriol. 1956 May;71(5):625–625. doi: 10.1128/jb.71.5.625-625.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  8. McGinnis M. R., Padhye A. A., Ajello L. Storage of stock cultures of filamentous fungi, yeasts, and some aerobic actinomycetes in sterile distilled water. Appl Microbiol. 1974 Aug;28(2):218–222. doi: 10.1128/am.28.2.218-222.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Salt D. T., Arret B., Wilner J. Low temperature maintenance of test organism suspensions for antibiotic assays. J Pharm Sci. 1973 Dec;62(12):2040–2043. doi: 10.1002/jps.2600621234. [DOI] [PubMed] [Google Scholar]

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