Abstract
Simple pour plate and spectrophotometric techniques for the evaluation of growth curves of several anaerobic bacteria on solid media are described. Three basic patterns of anaerobic growth were observed. The curves obtained were very reproducible when studied on separate occasions. The curves obtained by spectrophotometric measurement were comparable to those obtained by the pour plate method, especially when a large bacterial inoculum was used. Limitations in the interpretation of the results are discussed. The methods and principles reported could provide the basis for the determination of bacterial growth on solid media using other organisms and different experimental conditions.
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- Arank A., Syed S. A., Kenney E. B., Freter R. Isolation of anaerobic bacteria from human gingiva and mouse cecum by means of a simplified glove box procedure. Appl Microbiol. 1969 Apr;17(4):568–576. doi: 10.1128/am.17.4.568-576.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COLLINS F. M. THE EFFECT OF THE GROWTH RATE ON THE COMPOSITION OF S. ENTERITIDIS CELL WALLS. Aust J Exp Biol Med Sci. 1964 Apr;42:255–262. doi: 10.1038/icb.1964.27. [DOI] [PubMed] [Google Scholar]
- Caldwell D. R., Keeney M., Barton J. S., Kelley J. F. Sodium and other inorganic growth requirements of bacteroides amylophilus. J Bacteriol. 1973 May;114(2):782–789. doi: 10.1128/jb.114.2.782-789.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Czop J. K., Bergdoll M. S. Staphylococcal enterotoxin synthesis during the exponential, transitional, and stationary growth phases. Infect Immun. 1974 Feb;9(2):229–235. doi: 10.1128/iai.9.2.229-235.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loesche W. J. Oxygen sensitivity of various anaerobic bacteria. Appl Microbiol. 1969 Nov;18(5):723–727. doi: 10.1128/am.18.5.723-727.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MUSCHEL L. H., TREFFERS H. P. Quantitative studies on the bactericidal actions of serum and complement. I. A rapid photometric growth assay for bactericidal activity. J Immunol. 1956 Jan;76(1):1–10. [PubMed] [Google Scholar]
- SCHAECHTER M., MAALOE O., KJELDGAARD N. O. Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium. J Gen Microbiol. 1958 Dec;19(3):592–606. doi: 10.1099/00221287-19-3-592. [DOI] [PubMed] [Google Scholar]
- Wahren A., Holme T. Growth of Bacteroidaceae in stirred fermentors. Appl Microbiol. 1969 Aug;18(2):235–239. doi: 10.1128/am.18.2.235-239.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]