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. 1975 May;29(5):635–640. doi: 10.1128/am.29.5.635-640.1975

Isolation and Characterization of Vibrio parahaemolyticus from Cape Cod Soft-Shell Clams (Mya arenaria)

Paul M Earle 1, F D Crisley 1
PMCID: PMC187049  PMID: 1096817

Abstract

Vibro parahaemolyticus was isolated from soft-shell clams (Mya arenaria) taken from 10 different clamming areas on Cape Cod, Mass., during July and August 1972. Direct plating on thiosulfate-citrate-bile salts-sucrose agar was found to be superior to either direct plating on Vanderzant modified salt starch agar or enrichment with Trypticase soy broth containing 7% salt for isolation from clam samples. Morphological and biochemical characteristics of 33 isolates from 30 samples generally conform to those described for this organism in the literature, except for the production of acid from sucrose, lactose, and sorbitol. Six of the isolates were hemolytic on human blood agar plates, whereas all showed a negative Kanagawa phenomenon. Twenty of the 33 isolates reacted with pooled antisera to the K antigen; 15 of these reacted with 9 different specific K antisera, leaving 5 untypable. Ten of these 15 reacted with 4 different O antisera.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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