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. 1975 Jun;29(6):765–768. doi: 10.1128/am.29.6.765-768.1975

Comparative Inhibitory Effects of Antigen and Antibody in the Staphylococcal Enterotoxin Solid-Phase Radioimmunoassay System

J A Bukovic 1, H M Johnson 1, J T Peeler 1
PMCID: PMC187076  PMID: 1171653

Abstract

A solid-phase radioimmunoassay employing 125I-labeled enterotoxins and polystyrene tubes coated with specific antibody has been developed for assaying the relative concentrations of antibodies to staphylococcal enterotoxins A and B. Competitive binding occurs between tube-bound antibody and free antibody for binding sites on 125I-labeled enterotoxin. The sensitivity of the system is affected by the amount of antibody on the walls of the tubes, the concentration of 125I-labeled enterotoxin added to the system, and probably by the relative binding affinities of the bound and unbound antibodies. Antibody, 0.01 to 0.07 μg/ml, inhibited the uptake of 125I-labeled enterotoxin by 20%. Both the antibody and antigen solid-phase radioimmunoassay inhibition systems can be appropriately represented by either of the following two models: Loge (Y/1 - Y) = α0 + α1 LogeX and LogeY = β0 + β1 LogeX, where Y is bound activity, X is antigen or antibody concentration for inhibition, and α0, α1, β0, and β1 are regression coefficients. Estimates from the first model were slightly more precise for the antibody system, whereas the reverse was true for the antigen system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
  2. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HALL H. E., ANGELOTTI R., LEWIS K. H. QUANTITATIVE DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN B IN FOOD BY GEL-DIFFUSION METHODS. Public Health Rep. 1963 Dec;78:1089–1098. [PMC free article] [PubMed] [Google Scholar]
  4. Hunter R. Standardization of the chloramine-T method of protein iodination. Proc Soc Exp Biol Med. 1970 Mar;133(3):989–992. doi: 10.3181/00379727-133-34611. [DOI] [PubMed] [Google Scholar]
  5. Johnson H. M., Bukovic J. A., Kauffman P. E. Antigenic cross-reactivity of staphylococcal enterotoxins. Infect Immun. 1972 May;5(5):645–647. doi: 10.1128/iai.5.5.645-647.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Johnson H. M., Bukovic J. A., Kauffman P. E., Peeler J. T. Staphylococcal enterotoxin B: solid-phase radioimmunoassay. Appl Microbiol. 1971 Nov;22(5):837–841. doi: 10.1128/am.22.5.837-841.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rodbard D., Lewald J. E. Computer analysis of radioligand assay and radioimmunoassay data. Acta Endocrinol Suppl (Copenh) 1970;147:79–103. doi: 10.1530/acta.0.065s079. [DOI] [PubMed] [Google Scholar]

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