Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1975 Jun;29(6):807–813. doi: 10.1128/am.29.6.807-813.1975

Improved Medium for Lactic Streptococci and Their Bacteriophages

Betty E Terzaghi 1, W E Sandine 1,1
PMCID: PMC187084  PMID: 16350018

Abstract

Incorporation of 1.9% β-disodium glycerophosphate (GP) into a complex medium resulted in improved growth by lactic streptococci at 30 C. The medium, called M17, contained: Phytone peptone, 5.0 g; polypeptone, 5.0 g; yeast extract, 2.5 g; beef extract, 5.0 g; lactose, 5.0 g; ascorbic acid, 0.5 g; GP, 19.0 g; 1.0 M MgSO4·7H2O, 1.0 ml; and glass-distilled water, 1,000 ml. Based on absorbance readings and total counts, all strains of Streptococcus cremoris, S. diacetilactis, and S. lactis grew better in M17 medium than in a similar medium lacking GP or in lactic broth. Enhanced growth was probably due to the increased buffering capacity of the medium, since pH values below 5.70 were not reached after 24 h of growth at 30 C by S. lactis or S. cremoris strains. The medium also proved useful for isolation of bacterial mutants lacking the ability to ferment lactose; such mutants formed minute colonies on M17 agar plates, whereas wild-type cells formed colonies 3 to 4 mm in diameter. Incorporation of sterile GP into skim milk at 1.9% final concentration resulted in enhanced acid-producing activity by lactic streptococci when cells were inoculated from GP milk into skim milk not containing GP. M17 medium also proved superior to other media in demonstrating and distinguishing between lactic streptococcal bacteriophages. Plaques larger than 6 mm in diameter developed with some phage-host combinations, and turbid plaques, indicative of lysogeny, were also easily demonstrated for some systems.

Full text

PDF
807

Images in this article

811Image
on p.811
811Image
on p.811

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bertani G., Choe B. K., Lindahl G. Calcium sensitive and other mutants of bacteriophage P2. J Gen Virol. 1969 Jul;5(1):97–104. doi: 10.1099/0022-1317-5-1-97. [DOI] [PubMed] [Google Scholar]
  2. Bertani L. E., Bertani G. Preparation and characterization of temperate, non-inducible bacteriophage P2 (host: Escherichia coli). J Gen Virol. 1970 Feb;6(2):201–212. doi: 10.1099/0022-1317-6-2-201. [DOI] [PubMed] [Google Scholar]
  3. CITTI J. E., SANDINE W. E., ELLIKER P. R. COMPARISON OF SLOW AND FAST ACID-PRODUCING STREPTOCOCCUS LACTIS. J Dairy Sci. 1965 Jan;48:14–18. doi: 10.3168/jds.s0022-0302(65)88152-8. [DOI] [PubMed] [Google Scholar]
  4. Douglas J. A critical review of the use of glycerophosphates in microbiological media. Lab Pract. 1971 May;20(5):414–passim. [PubMed] [Google Scholar]
  5. Douglas J., Qanber-Agha A., Phillips V. Medium for the propagation and assay of lactic and other phages. Lab Pract. 1974 Jan;23(1):3–4. [PubMed] [Google Scholar]
  6. Kozak W., Rajchert-Trzpil M., Zajdel J., Dobrzański W. T. Lysogeny in lactic streptococci producing and not producing nisin. Appl Microbiol. 1973 Feb;25(2):305–308. doi: 10.1128/am.25.2.305-308.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lowrie R. J. Lysogenic strains of group N lactic streptococci. Appl Microbiol. 1974 Jan;27(1):210–217. doi: 10.1128/am.27.1.210-217.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McKay L. L., Baldwin K. A., Zottola E. A. Loss of lactose metabolism in lactic streptococci. Appl Microbiol. 1972 Jun;23(6):1090–1096. doi: 10.1128/am.23.6.1090-1096.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Molskness T. A., Sandine W. E., Brown L. R. Characterization of lac+ transductants of Streptococcus lactis. Appl Microbiol. 1974 Nov;28(5):753–758. doi: 10.1128/am.28.5.753-758.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nyiendo J., Seidler R. J., Sandine W. E., Elliker P. R. Preparation and storage of high-titer lactic streptococcus bacteriophages. Appl Microbiol. 1974 Jan;27(1):72–77. doi: 10.1128/am.27.1.72-77.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pearce L. E., Skipper N. A., Jarvis B. D. Proteinase activity in slow lactic acid-producing variants of Streptococcus lactis. Appl Microbiol. 1974 May;27(5):933–937. doi: 10.1128/am.27.5.933-937.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Thomas T. D., Jarvis B. D., Skipper N. A. Localization of proteinase(s) near the cell surface of Streptococcus lactis. J Bacteriol. 1974 May;118(2):329–333. doi: 10.1128/jb.118.2.329-333.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES