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. 1975 Aug;30(2):267–270. doi: 10.1128/am.30.2.267-270.1975

Chemostat Culture of Escherichia coli K-12 Limited by the Activity of Alkaline Phosphatase

Stagg L King 1, J C Francis 1
PMCID: PMC187165  PMID: 240310

Abstract

The growth-limiting reaction of a chemostat culture of Escherichia coli K-12 was the hydrolysis of β-glycerophosphate by alkaline phosphatase. The culture was buffered at pH 5.2 where alkaline phosphatase was unable to supply phosphate to the cell at a rate sufficient to sustain the maximum rate of growth. Alkaline phosphatase activity in this system is discussed in terms of the so-called Flip-Flop mechanism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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