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. 1975 Aug;30(2):271–275. doi: 10.1128/am.30.2.271-275.1975

Derivation of High Enterotoxin B-Producing Mutants of Staphylococcus aureus from the Parent Strains

Robert A Altenbern 1
PMCID: PMC187166  PMID: 240311

Abstract

Certain pH-sensitive (membrane) mutants of Staphylococcus aureus, strains 14458 and 778, produce significantly more type-B enterotoxin (SEB) than the parent type. Some carbohydrate mutants (car) from these parent strains also are superior to the parent in SEB formation. By isolating car mutants from high-SEB-producing membrane mutants, it is possible to derive a double mutant producing from six to 50 times as much SEB as the parent type. Inversion of the sequence by isolating pH-sensitive mutants from car mutants does not yield clones with strikingly higher SEB production than the parent strain. The successful isolation sequence (pH-sensitive mutant first and car mutants derived from it) is relatively simple and virtually assures detection of a truly high-SEB-producing clone. The total number of clones whose direct assay for SEB formation is necessary for detection of a high-producing mutant is on the order of 50 to 60.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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