Abstract
Thirty-one soil samples were examined for the presence of organisms capable of inhibiting growth and toxin production of strains of Clostridium botulinum type A. Such organisms were found in eight samples of soil. Inhibiting strains of C. perfringens were found in five samples, of C. sporogenes in three and of Bacillus cereus in three. Three of the C. perfringens strains produced an inhibitor effective on all 11 strains of C. botulinum type A against which they were tested, seven of eight proteolytic type B strains, one nonproteolytic type B strain, five of nine type E strains and all seven type F strains, whether proteolytic or nonproteolytic. They did not inhibit any of 26 type C strains, 6 type D strains, 4 type E strains, or 24 C. sporogenes strains. In mixed culture, an inhibitor strain of C. perfringens repressed growth and toxin production by a C. botulinum type A strain even though it was outnumbered by the latter about 40 times. It also repressed growth and toxin production of C. botulinum in mixed culture of soils in which this latter organism naturally occurred when cooked meat medium but not when trypticase medium was used.
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- Anastasio K. L., Soucheck J. A., Sugiyama H. Boticinogeny and actions of the bacteriocin. J Bacteriol. 1971 Jul;107(1):143–149. doi: 10.1128/jb.107.1.143-149.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bott T. L., Johnson J., Jr, Foster E. M., Sugiyama H. Possible origin of the high incidence of Clostridium botulinum type E in an inland bay (Green Bay of Lake Michigan). J Bacteriol. 1968 May;95(5):1542–1547. doi: 10.1128/jb.95.5.1542-1547.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clarke D. J., Robson R. M., Morris J. G. Purification of two Clostridium bacteriocins by procedures appropriate to hydrophobic proteins. Antimicrob Agents Chemother. 1975 Mar;7(3):256–264. doi: 10.1128/aac.7.3.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duncan C. L., Strong D. H. Improved medium for sporulation of Clostridium perfringens. Appl Microbiol. 1968 Jan;16(1):82–89. doi: 10.1128/am.16.1.82-89.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ellison J. S., Kautter J. A. Purification and some properties of two boticins. J Bacteriol. 1970 Oct;104(1):19–26. doi: 10.1128/jb.104.1.19-26.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kautter D. A., Harmon S. M., Lynt R. K., Jr, Lilly T., Jr Antagonistic effect on Clostridium botulinum type E by organisms resembling it. Appl Microbiol. 1966 Jul;14(4):616–622. doi: 10.1128/am.14.4.616-622.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee W. H., Riemann H. Correlation of toxic and non-toxic strains of Clostridium botulinum by DNA composition and homology. J Gen Microbiol. 1970 Jan;60(1):117–123. doi: 10.1099/00221287-60-1-117. [DOI] [PubMed] [Google Scholar]
- MANDIA J. W. Serological group II of the proteolytic Clostridia. J Immunol. 1951 Jul;67(1):49–55. [PubMed] [Google Scholar]
- Midura T. F., Inouye Y., Bodily H. L. Use of immunofluorescence to identify Clostridium botulinum types A, B, and E. Public Health Rep. 1967 Mar;82(3):275–279. [PMC free article] [PubMed] [Google Scholar]
- Ward B. Q., Carroll B. J., Garrett E. S., Reese G. B. Survey of the U.S. Gulf Coast for the presence of Clostridium botulinum. Appl Microbiol. 1967 May;15(3):629–636. doi: 10.1128/am.15.3.629-636.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wentz M. W., Scott R. A., Vennes J. W. Clostridium botulinum type F: seasonal inhibition by bacillus licheniformis. Science. 1967 Jan 6;155(3758):89–90. doi: 10.1126/science.155.3758.89. [DOI] [PubMed] [Google Scholar]