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. 1975 Nov;30(5):825–830. doi: 10.1128/am.30.5.825-830.1975

Development of a Micromethod for Identification of Anaerobic Bacteria

Tracy D Wilkins 1, Clay B Walker 1
PMCID: PMC187279  PMID: 1106324

Abstract

A microprocedure was described for determining the carbohydrate fermentation patterns of 48 anaerobic bacteria at one time in microtiter plates. The cultures were transferred into agar-filled wells of microtiter plates with a replicator inside an anaerobic glove box. Fermentation was measured both with a colorimetric indicator and with a small pH electrode. The method was approximately 97% accurate. It would be most useful for laboratories that need to identify large numbers of anaerobes at one time.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aranki A., Freter R. Use of anaerobic glove boxes for the cultivation of strictly anaerobic bacteria. Am J Clin Nutr. 1972 Dec;25(12):1329–1334. doi: 10.1093/ajcn/25.12.1329. [DOI] [PubMed] [Google Scholar]
  2. Moore W. E., Holdeman L. V. Human fecal flora: the normal flora of 20 Japanese-Hawaiians. Appl Microbiol. 1974 May;27(5):961–979. doi: 10.1128/am.27.5.961-979.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Wilkins T. D., Walker C. B., Moore W. E. Micromethod for identification of anaerobic bacteria: design and operation of apparatus. Appl Microbiol. 1975 Nov;30(5):831–837. doi: 10.1128/am.30.5.831-837.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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