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. 1993 Jan;37(1):148–151. doi: 10.1128/aac.37.1.148

Neutralizing antibodies against the peptide antibiotic AS-48: immunocytological studies.

M Maqueda 1, A Gálvez 1, M Martínez-Bueno 1, I Guerra 1, E Valdivia 1
PMCID: PMC187625  PMID: 8431014

Abstract

Antisera against the broad-spectrum peptide antibiotic AS-48 produced by Enterococcus faecalis were obtained from immunized rabbits. Appreciable antibody titers were obtained only after repeated immunization, suggesting a feeble antigenicity for AS-48. Upon incubation with AS-48, the antisera neutralized its bacteriolytic action on E. faecalis S-47, although the simultaneous addition of AS-48 and serum did not prevent lysis. Crude serum cross-reacted with outer envelope components of enterococci, although specific anti-AS-48 antibodies, purified by affinity chromatography, reacted only with AS-48-treated cells. Labelling with immunofluorescence and colloidal gold particles was carried out on sensitive and resistant bacterial species to determine the interaction of AS-48 with cell structures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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