Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1990 May;136(5):1147–1152.

Expression of Zn-alpha 2-glycoprotein and PSP-94 in prostatic adenocarcinoma. An immunohistochemical study of 88 cases.

S Gagnon 1, B Têtu 1, J Y Dubé 1, R R Tremblay 1
PMCID: PMC1877423  PMID: 2349965

Abstract

Zn-Alpha 2-Glycoprotein (Zn-Alpha 2-GP) and prostatic secretory protein of 94 amino-acids (PSP-94) were recently isolated from the human prostate. Their expression in benign and malignant well-differentiated and poorly differentiated components of 88 prostates with prostatic adenocarcinomas, and in 25 metastases, was evaluated using polyclonal antibodies developed against these antigens. Zn-Alpha 2-GP was present in benign hyperplastic glands in 91.1% of cases, but in only 40.7% (poorly differentiated component) to 48.5% (well-differentiated component) of prostatic adenocarcinomas, and in 8% of metastases. The expression of PSP-94 was present in 89.3% of benign hyperplastic glands, but in only 50% (well-differentiated adenocarcinoma component) to 57.3% (poorly differentiated component) of prostatic adenocarcinomas and 28% of metastases. The expression of these proteins by the tumor was unrelated to the initial stage and the tumor grade. Because of their low frequency in prostatic adenocarcinomas, especially in metastases, Zn-Alpha 2-GP and PSP-94 appear to have a limited diagnostic usefulness. Further studies are needed, however, to explore other clinical applications of these two new prostatic secretory proteins.

Full text

PDF
1147

Images in this article

1149Figure 1
on p.1149
1150Figure 2
on p.1150
1150Figure 3
on p.1150
1151Figure 4
on p.1151

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Al Adnani M. S. Origin of carcinomas causing bladder neck obstruction demonstrated by immunoperoxidase localisation of specific antigens. Br J Urol. 1986 Jun;58(3):283–286. doi: 10.1111/j.1464-410x.1986.tb09055.x. [DOI] [PubMed] [Google Scholar]
  2. Allhoff E. P., Proppe K. H., Chapman C. M., Lin C. W., Prout G. R., Jr Evaluation of prostate specific acid phosphatase and prostate specific antigen in identification of prostatic cancer. J Urol. 1983 Feb;129(2):315–318. doi: 10.1016/s0022-5347(17)52074-1. [DOI] [PubMed] [Google Scholar]
  3. BURGI W., SCHMID K. Preparation and properties of Zn-alpha 2-glycoprotein of normal human plasma. J Biol Chem. 1961 Apr;236:1066–1074. [PubMed] [Google Scholar]
  4. Brawn P. N., Ayala A. G., Von Eschenbach A. C., Hussey D. H., Johnson D. E. Histologic grading study of prostate adenocarcinoma: the development of a new system and comparison with other methods--a preliminary study. Cancer. 1982 Feb 1;49(3):525–532. doi: 10.1002/1097-0142(19820201)49:3<525::aid-cncr2820490321>3.0.co;2-m. [DOI] [PubMed] [Google Scholar]
  5. Carter D. B., Resnick M. I. High resolution analysis of human prostatic fluid by two-dimensional electrophoresis. Prostate. 1982;3(1):27–33. doi: 10.1002/pros.2990030106. [DOI] [PubMed] [Google Scholar]
  6. Chalbos D., Haagensen D., Parish T., Rochefort H. Identification and androgen regulation of two proteins released by T47D human breast cancer cells. Cancer Res. 1987 Jun 1;47(11):2787–2792. [PubMed] [Google Scholar]
  7. Doctor V. M., Sheth A. R., Simha M. M., Arbatti N. J., Aaveri J. P., Sheth N. A. Studies on immunocytochemical localization of inhibin-like material in human prostatic tissue: comparison of its distribution in normal, benign and malignant prostates. Br J Cancer. 1986 Apr;53(4):547–554. doi: 10.1038/bjc.1986.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dube V. E., Farrow G. M., Greene L. F. Prostatic adenocarcinoma of ductal origin. Cancer. 1973 Aug;32(2):402–409. doi: 10.1002/1097-0142(197308)32:2<402::aid-cncr2820320218>3.0.co;2-q. [DOI] [PubMed] [Google Scholar]
  9. Dubé J. Y., Frenette G., Paquin R., Chapdelaine P., Tremblay J., Tremblay R. R., Lazure C., Seidah N., Chrétien M. Isolation from human seminal plasma of an abundant 16-kDa protein originating from the prostate, its identification with a 94-residue peptide originally described as beta-inhibin. J Androl. 1987 May-Jun;8(3):182–189. doi: 10.1002/j.1939-4640.1987.tb02429.x. [DOI] [PubMed] [Google Scholar]
  10. Dubé J. Y., Pelletier G., Gagnon P., Tremblay R. R. Immunohistochemical localization of a prostatic secretory protein of 94 amino acids in normal prostatic tissue, in primary prostatic tumors and in their metastases. J Urol. 1987 Oct;138(4):883–887. doi: 10.1016/s0022-5347(17)43408-2. [DOI] [PubMed] [Google Scholar]
  11. Frenette G., Dubé J. Y., Lazure C., Paradis G., Chrétien M., Tremblay R. R. The major 40-kDa glycoprotein in human prostatic fluid is identical to Zn-alpha 2-glycoprotein. Prostate. 1987;11(3):257–270. doi: 10.1002/pros.2990110306. [DOI] [PubMed] [Google Scholar]
  12. Greene L. F., Farrow G. M., Ravits J. M., Tomera F. M. Prostatic adenocarcinoma of ductal origin. J Urol. 1979 Mar;121(3):303–305. doi: 10.1016/s0022-5347(17)56763-4. [DOI] [PubMed] [Google Scholar]
  13. Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
  14. Jewett H. J. The present status of radical prostatectomy for stages A and B prostatic cancer. Urol Clin North Am. 1975 Feb;2(1):105–124. [PubMed] [Google Scholar]
  15. Li C. Y., Lam W. K., Yam L. T. Immunohistochemical diagnosis of prostatic cancer with metastasis. Cancer. 1980 Aug 15;46(4):706–712. doi: 10.1002/1097-0142(19800815)46:4<706::aid-cncr2820460412>3.0.co;2-e. [DOI] [PubMed] [Google Scholar]
  16. Lin M. F., Lee C. L., Sharief F. S., Li S. S., Chu T. M. Glycoprotein exhibiting immunological and enzymatic activities of human prostatic acid phosphatase. Cancer Res. 1983 Aug;43(8):3841–3846. [PubMed] [Google Scholar]
  17. Nadji M., Tabei S. Z., Castro A., Chu T. M., Murphy G. P., Wang M. C., Morales A. R. Prostatic-specific antigen: an immunohistologic marker for prostatic neoplasms. Cancer. 1981 Sep 1;48(5):1229–1232. doi: 10.1002/1097-0142(19810901)48:5<1229::aid-cncr2820480529>3.0.co;2-l. [DOI] [PubMed] [Google Scholar]
  18. Papsidero L. D., Croghan G. A., Asirwatham J., Gaeta J., Abenoza P., Englander L., Valenzuela L. Immunohistochemical demonstration of prostate-specific antigen in metastases with the use of monoclonal antibody F5. Am J Pathol. 1985 Dec;121(3):451–454. [PMC free article] [PubMed] [Google Scholar]
  19. Ro J. Y., Ayala A. G., Wishnow K. I., Ordóez N. G. Prostatic duct adenocarcinoma with endometrioid features: immunohistochemical and electron microscopic study. Semin Diagn Pathol. 1988 Aug;5(3):301–311. [PubMed] [Google Scholar]
  20. Rui H., Mevåg B., Purvis K. Two-dimensional electrophoresis of proteins in various fractions of the human split ejaculate. Int J Androl. 1984 Dec;7(6):509–520. doi: 10.1111/j.1365-2605.1984.tb00808.x. [DOI] [PubMed] [Google Scholar]
  21. Sheth N. A., Doctor V. M., Sampat M. B., Garde S. V., Arbatti N. J., Sheth A. R. Inhibin-like material--an immunohistologic marker for prostatic origin of metastases. Cancer Lett. 1987 Jul;36(1):93–98. doi: 10.1016/0304-3835(87)90106-6. [DOI] [PubMed] [Google Scholar]
  22. Thakur A. N., Vaze A. Y., Dattatreyamurthy B., Sheth A. R. Isolation & characterization of inhibin from human seminal plasma. Indian J Exp Biol. 1981 Apr;19(4):307–313. [PubMed] [Google Scholar]
  23. Tsai Y. C., Harrison H. H., Lee C., Daufeldt J. A., Oliver L., Grayhack J. T. Systematic characterization of human prostatic fluid proteins with two-dimensional electrophoresis. Clin Chem. 1984 Dec;30(12 Pt 1):2026–2030. [PubMed] [Google Scholar]
  24. Wang M. C., Valenzuela L. A., Murphy G. P., Chu T. M. Purification of a human prostate specific antigen. Invest Urol. 1979 Sep;17(2):159–163. [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES