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. 1990 Jan;136(1):13–16.

Colonic aberrant crypts in azoxymethane-treated F344 rats have decreased hexosaminidase activity.

T P Pretlow 1, M A O'Riordan 1, M F Kolman 1, J A Jurcisek 1
PMCID: PMC1877465  PMID: 2297043

Abstract

Aberrant crypts, identified with methylene blue staining of unsectioned colon from carcinogen-treated rats on the basis of their increased size, were examined for the altered expression of hexosaminidase activity. Previously we identified enzyme-altered foci with normal morphology in sections of colon from carcinogen-treated rats. A reduction of histochemically demonstrable hexosaminidase activity was the most consistent marker for these foci. Aberrant crypts, marked with permanent ink and embedded in methacrylate, had a marked decrease of hexosaminidase activity compared to the adjacent, normal crypts. Hexosaminidase may be a marker that will aid in the identification of the molecular basis of colon cancer in a manner similar to that of esterase D and retinoblastoma.

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Selected References

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