Abstract
The authors have used morphometric, immunocytochemical, and electron optical techniques to study fibrin deposits associated with villi from 14 normal term placentas, and have examined the response of cultured cellular trophoblast to fibrin matrix in vitro. Morphometric analysis of 3477 villous profiles showed that 5.5% of villi examined had fibrin deposition at sites of syncytial denudation and that fibrin deposition was highly associated with villous epithelial denudation, as evidenced by loss of cytokeratin staining. The perivillous fibrin deposits were strongly immunoreactive for the B beta chain of fibrin II, consistent with local thrombolytic cleavage of fibrinogen to fibrin. Deposits were frequently surfaced by a discontinuous layer of cytokeratin-positive trophoblastic cells that showed type IV basement membrane collagen immunoreactivity at the interface between trophoblast and fibrin. Ultrastructurally, damage to the syncytial trophoblast was apparent at the edge of some deposits, where syncytial denudation was accompanied by a fibrin coating of residual cellular trophoblast and the trophoblastic basal lamina. Other deposits were surfaced by syncytial trophoblast with underlying cellular trophoblast and a new basal lamina external to the basal lamina of the villous core. Cultured cellular trophoblast grown on a fibrin matrix, but not on uncoated plastic, showed morphologic differentiation into a trophoblast layer like that on term villi. The authors suggest that epithelialization of perivillous fibrin deposits is a form of villous repair and that trophoblast-fibrin interactions can modulate trophoblastic differentiation.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Enders A. C. Fine structure of anchoring villi of the human placenta. Am J Anat. 1968 May;122(3):419–451. doi: 10.1002/aja.1001220302. [DOI] [PubMed] [Google Scholar]
- Fox H. Fibrinoid necrosis of placental villi. J Obstet Gynaecol Br Commonw. 1968 Apr;75(4):448–452. doi: 10.1111/j.1471-0528.1968.tb00142.x. [DOI] [PubMed] [Google Scholar]
- Fox H. Perivillous fibrin deposition in the human placenta. Am J Obstet Gynecol. 1967 May 15;98(2):245–251. doi: 10.1016/s0002-9378(16)34594-x. [DOI] [PubMed] [Google Scholar]
- Hamilton W. J., Boyd J. D. Specializations of the syncytium of the human chorion. Br Med J. 1966 Jun 18;1(5502):1501–1506. doi: 10.1136/bmj.1.5502.1501. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones M., Gabriel D. A. Influence of the subendothelial basement membrane components on fibrin assembly. Evidence for a fibrin binding site on type IV collagen. J Biol Chem. 1988 May 25;263(15):7043–7048. [PubMed] [Google Scholar]
- Kleinman H. K., Luckenbill-Edds L., Cannon F. W., Sephel G. C. Use of extracellular matrix components for cell culture. Anal Biochem. 1987 Oct;166(1):1–13. doi: 10.1016/0003-2697(87)90538-0. [DOI] [PubMed] [Google Scholar]
- Kliman H. J., Nestler J. E., Sermasi E., Sanger J. M., Strauss J. F., 3rd Purification, characterization, and in vitro differentiation of cytotrophoblasts from human term placentae. Endocrinology. 1986 Apr;118(4):1567–1582. doi: 10.1210/endo-118-4-1567. [DOI] [PubMed] [Google Scholar]
- Kudryk B., Rohoza A., Ahadi M., Chin J., Wiebe M. E. Specificity of a monoclonal antibody for the NH2-terminal region of fibrin. Mol Immunol. 1984 Jan;21(1):89–94. doi: 10.1016/0161-5890(84)90093-2. [DOI] [PubMed] [Google Scholar]
- Moe N. Histological and histochemical study of the extracellular deposits in the normal human placenta. Acta Pathol Microbiol Scand. 1969;76(3):419–431. doi: 10.1111/j.1699-0463.1969.tb03272.x. [DOI] [PubMed] [Google Scholar]
- Moe N., Jorgensen L. Fibrin deposits on the syncytium of the normal human placenta: evidence of their thrombogenic origin. Acta Pathol Microbiol Scand. 1968;72(4):519–541. doi: 10.1111/j.1699-0463.1968.tb00466.x. [DOI] [PubMed] [Google Scholar]
- Moe N. The deposits of fibrin and fibrin-like materials in the basal plate of the normal human placenta. Acta Pathol Microbiol Scand. 1969;75(1):1–17. [PubMed] [Google Scholar]
- The association of clinical pathologists: 75th general meeting. J Clin Pathol. 1966 Jan;19(1):98–102. doi: 10.1136/jcp.19.1.98. [DOI] [PMC free article] [PubMed] [Google Scholar]