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. 1990 Dec;137(6):1425–1434.

Production of Transforming Growth Factor Alpha by Hamster Eosinophils

Aram Elovic, Stephen J Galli, Peter F Weller, Allen L C Chang, Tao Chiang, Ming Yung Chou, R Bruce Donoff, George T Gallagher, Karekine Matossian, Jim McBride, Mindy Tsai, Randy Todd, David T W Wong
PMCID: PMC1877717  PMID: 2124414

Abstract

Previously it was demonstrated that malignant transformation of the Syrian hamster cheek pouchmucosa is associated with the expression of TGF-α. Therefore in situ hybridization and immunohistochemistry was used to investigate the cellular sources of TGF-α production in this model system. Surprisingly one cell type in the inflammatory infiltrate present in the connective tissue adjacent to the transformed epithelium represented a major source of TGF-α mRNA. Detailed analysis of these cells revealed that they were eosinophils. In addition to TGF-α mRNA, about 40% of the eosinophils associated with the oral tumors exhibited TGF-α product reactive with a monoclonal antibody against the C terminus of the mature TGF-α peptide. Normal hamster bone marrow eosinophils also exhibited TGF-α mRNA and product by in situ hybridization and immunohistochemistry. These results suggest that the eosinophil represents a biologically significant source of TGF-α.

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Selected References

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