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. 1993 May;37(5):1167–1170. doi: 10.1128/aac.37.5.1167

Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants.

N B Murphy 1, A M Muthiani 1, A S Peregrine 1
PMCID: PMC187924  PMID: 8517708

Abstract

We determined the level of susceptibility of Trypanosoma brucei brucei to the aminoglycoside G418 in vivo and demonstrated that it is possible to select for G418-resistant transfected T. brucei brucei bloodstream parasites in a mouse host by inoculating the drug intraperitoneally at doses between 40 and 80 mg/kg of body weight daily for 3 days. The ability to select for transfectants in vivo offers new possibilities for studies on genetic recombination in these parasites.

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Selected References

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