Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1989 Mar;134(3):651–659.

In situ demonstration of Epstein-Barr viral genomes in viral-associated B cell lymphoproliferations.

L M Weiss 1, L A Movahed 1
PMCID: PMC1879531  PMID: 2538060

Abstract

Thirteen cases of benign and malignant Epstein-Barr viral (EBV)-associated B cell lymphoproliferations were examined by in situ hybridization studies performed on formalin-fixed, paraffin-embedded tissue sections. EBV nucleic acids were identified in a minority of lymphoid cells in five of six cases of benign infectious mononucleosis studied in tonsil or lymph node specimens. No evidence of EBV was found in two splenectomy specimens from patients with infectious mononucleosis. EBV nucleic acids were identified in one case of fatal, infectious, mononucleosislike immunoblastic proliferation, and were especially concentrated in areas where there were sheets of immunoblasts associated with necrosis. EBV nucleic acids were identified in all four cases of EBV-associated post-transplant lymphoproliferations, including three cases of non-Hodgkin's lymphoma in which a majority of the neoplastic cells contained EBV nucleic acids.

Full text

PDF
651

Images in this article

654Figure 1
on p.654
655Figure 2
on p.655
655Figure 3
on p.655
656Figure 4
on p.656
656Figure 5
on p.656
657Figure 6
on p.657

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andiman W., Gradoville L., Heston L., Neydorff R., Savage M. E., Kitchingman G., Shedd D., Miller G. Use of cloned probes to detect Epstein-Barr viral DNA in tissues of patients with neoplastic and lymphoproliferative diseases. J Infect Dis. 1983 Dec;148(6):967–977. doi: 10.1093/infdis/148.6.967. [DOI] [PubMed] [Google Scholar]
  2. Cheung A., Kieff E. Long internal direct repeat in Epstein-Barr virus DNA. J Virol. 1982 Oct;44(1):286–294. doi: 10.1128/jvi.44.1.286-294.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Childs C. C., Parham D. M., Berard C. W. Infectious mononucleosis. The spectrum of morphologic changes simulating lymphoma in lymph nodes and tonsils. Am J Surg Pathol. 1987 Feb;11(2):122–132. doi: 10.1097/00000478-198702000-00006. [DOI] [PubMed] [Google Scholar]
  4. Crum C. P., Nagai N., Levine R. U., Silverstein S. In situ hybridization analysis of HPV 16 DNA sequences in early cervical neoplasia. Am J Pathol. 1986 Apr;123(1):174–182. [PMC free article] [PubMed] [Google Scholar]
  5. Crum C. P., Nuovo G., Friedman D., Silverstein S. J. A comparison of biotin and isotope-labeled ribonucleic acid probes for in situ detection of HPV-16 ribonucleic acid in genital precancers. Lab Invest. 1988 Mar;58(3):354–359. [PubMed] [Google Scholar]
  6. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  7. Frizzera G., Hanto D. W., Gajl-Peczalska K. J., Rosai J., McKenna R. W., Sibley R. K., Holahan K. P., Lindquist L. L. Polymorphic diffuse B-cell hyperplasias and lymphomas in renal transplant recipients. Cancer Res. 1981 Nov;41(11 Pt 1):4262–4279. [PubMed] [Google Scholar]
  8. Hanto D. W., Frizzera G., Gajl-Peczalska K. J., Simmons R. L. Epstein-Barr virus, immunodeficiency, and B cell lymphoproliferation. Transplantation. 1985 May;39(5):461–472. doi: 10.1097/00007890-198505000-00001. [DOI] [PubMed] [Google Scholar]
  9. Hanto D. W., Sakamoto K., Purtilo D. T., Simmons R. L., Najarian J. S. The Epstein-Barr virus in the pathogenesis of posttransplant lymphoproliferative disorders. Clinical, pathologic, and virologic correlation. Surgery. 1981 Aug;90(2):204–213. [PubMed] [Google Scholar]
  10. Jones J. F., Shurin S., Abramowsky C., Tubbs R. R., Sciotto C. G., Wahl R., Sands J., Gottman D., Katz B. Z., Sklar J. T-cell lymphomas containing Epstein-Barr viral DNA in patients with chronic Epstein-Barr virus infections. N Engl J Med. 1988 Mar 24;318(12):733–741. doi: 10.1056/NEJM198803243181203. [DOI] [PubMed] [Google Scholar]
  11. Krueger G. R., Papadakis T., Schaefer H. J. Persistent active Epstein-Barr virus infection and atypical lymphoproliferation. Report of two cases. Am J Surg Pathol. 1987 Dec;11(12):972–981. doi: 10.1097/00000478-198712000-00008. [DOI] [PubMed] [Google Scholar]
  12. Lawrence J. B., Villnave C. A., Singer R. H. Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma line. Cell. 1988 Jan 15;52(1):51–61. doi: 10.1016/0092-8674(88)90530-2. [DOI] [PubMed] [Google Scholar]
  13. Masih A. S., Linder J., Shaw B. W., Jr, Wood R. P., Donovan J. P., White R., Markin R. S. Rapid identification of cytomegalovirus in liver allograft biopsies by in situ hybridization. Am J Surg Pathol. 1988 May;12(5):362–367. doi: 10.1097/00000478-198805000-00004. [DOI] [PubMed] [Google Scholar]
  14. Rentrop M., Knapp B., Winter H., Schweizer J. Aminoalkylsilane-treated glass slides as support for in situ hybridization of keratin cDNAs to frozen tissue sections under varying fixation and pretreatment conditions. Histochem J. 1986 May;18(5):271–276. doi: 10.1007/BF01676237. [DOI] [PubMed] [Google Scholar]
  15. Sauerbrei A., Wutzler P., Färber I., Brichácek B., Swoboda R., Macheleidt S. Comparative detection of herpesviruses in tissue specimens by in situ hybridization and immunofluorescence. Acta Virol. 1986 May;30(3):213–219. [PubMed] [Google Scholar]
  16. Sixbey J. W., Nedrud J. G., Raab-Traub N., Hanes R. A., Pagano J. S. Epstein-Barr virus replication in oropharyngeal epithelial cells. N Engl J Med. 1984 May 10;310(19):1225–1230. doi: 10.1056/NEJM198405103101905. [DOI] [PubMed] [Google Scholar]
  17. Sixbey J. W., Vesterinen E. H., Nedrud J. G., Raab-Traub N., Walton L. A., Pagano J. S. Replication of Epstein-Barr virus in human epithelial cells infected in vitro. Nature. 1983 Dec 1;306(5942):480–483. doi: 10.1038/306480a0. [DOI] [PubMed] [Google Scholar]
  18. Sullivan J. L. Epstein-Barr virus and the X-linked lymphoproliferative syndrome. Adv Pediatr. 1983;30:365–399. [PubMed] [Google Scholar]
  19. Syrjänen S., Laine P., Valle S. L. Demonstration of Epstein-Barr virus (EBV) DNA in oral hairy leukoplakia using in situ hybridization with biotinylated probe. Proc Finn Dent Soc. 1988;84(2):127–132. [PubMed] [Google Scholar]
  20. Teo C. G., Griffin B. E. Epstein-Barr virus genomes in lymphoid cells: activation in mitosis and chromosomal location. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8473–8477. doi: 10.1073/pnas.84.23.8473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Unger E. R., Budgeon L. R., Myerson D., Brigati D. J. Viral diagnosis by in situ hybridization. Description of a rapid simplified colorimetric method. Am J Surg Pathol. 1986 Jan;10(1):1–8. doi: 10.1097/00000478-198601000-00001. [DOI] [PubMed] [Google Scholar]
  22. Weiss L. M., Strickler J. G., Warnke R. A., Purtilo D. T., Sklar J. Epstein-Barr viral DNA in tissues of Hodgkin's disease. Am J Pathol. 1987 Oct;129(1):86–91. [PMC free article] [PubMed] [Google Scholar]
  23. Wolf H., Haus M., Wilmes E. Persistence of Epstein-Barr virus in the parotid gland. J Virol. 1984 Sep;51(3):795–798. doi: 10.1128/jvi.51.3.795-798.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yamashiroya H. M., Ghosh L., Yang R., Robertson A. L., Jr Herpesviridae in the coronary arteries and aorta of young trauma victims. Am J Pathol. 1988 Jan;130(1):71–79. [PMC free article] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES