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. 1989 Feb;134(2):243–251.

Beta-protein amyloid is widely distributed in the central nervous system of patients with Alzheimer's disease.

K Ogomori 1, T Kitamoto 1, J Tateishi 1, Y Sato 1, M Suetsugu 1, M Abe 1
PMCID: PMC1879581  PMID: 2464938

Abstract

To clarify the distribution, morphology, and density of amyloid deposits in patients with Alzheimer's disease (AD), tissue sections from various areas of the central nervous system of 14 patients with AD and from 20 nondemented aged controls were investigated immunohistochemically using anti-beta protein antiserum. beta-protein amyloid deposits were present not only in the cores of the senile plaques and in the vascular wall (amyloid angiopathy), but also in various sized plaque-shaped fibrillary, perivascular, subpial, and subependymal deposits. Amyloid deposits were found mainly in the cerebral cortex in nondemented controls, while in AD they were distributed widely in the regions that were not affected in nondemented controls. The positivity of amyloid deposits in AD was 100% in the cerebral cortex, hippocampus, amygdala, thalamus, caudate nucleus, claustrum, hypothalamus, nucleus basalis of Meynert, and cerebellar cortex. Putamen and brain-stem nuclei were affected frequently, and the spinal cord, dentate nucleus, and globus pallidus were sometimes (less than 50%) affected. This result provides an evidence that Alzheimer's disease is a beta-protein amyloidosis of the central nervous system. An assessment of the distribution of amyloid deposits should prove to be useful for the histopathologic diagnosis of AD.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S., Taudou B., Chuilon S. Glutaraldehyde, cyanuric chloride and tetrazotized O-dianisidine as coupling reagents in the passive hemagglutination test. Immunochemistry. 1969 Jan;6(1):67–76. doi: 10.1016/0019-2791(69)90179-7. [DOI] [PubMed] [Google Scholar]
  2. Avrameas S., Ternynck T. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry. 1969 Jan;6(1):53–66. doi: 10.1016/0019-2791(69)90178-5. [DOI] [PubMed] [Google Scholar]
  3. Blessed G., Tomlinson B. E., Roth M. The association between quantitative measures of dementia and of senile change in the cerebral grey matter of elderly subjects. Br J Psychiatry. 1968 Jul;114(512):797–811. doi: 10.1192/bjp.114.512.797. [DOI] [PubMed] [Google Scholar]
  4. Coria F., Castaño E. M., Frangione B. Brain amyloid in normal aging and cerebral amyloid angiopathy is antigenically related to Alzheimer's disease beta-protein. Am J Pathol. 1987 Dec;129(3):422–428. [PMC free article] [PubMed] [Google Scholar]
  5. Glenner G. G., Wong C. W. Alzheimer's disease: initial report of the purification and characterization of a novel cerebrovascular amyloid protein. Biochem Biophys Res Commun. 1984 May 16;120(3):885–890. doi: 10.1016/s0006-291x(84)80190-4. [DOI] [PubMed] [Google Scholar]
  6. Goldgaber D., Lerman M. I., McBride O. W., Saffiotti U., Gajdusek D. C. Characterization and chromosomal localization of a cDNA encoding brain amyloid of Alzheimer's disease. Science. 1987 Feb 20;235(4791):877–880. doi: 10.1126/science.3810169. [DOI] [PubMed] [Google Scholar]
  7. HIRANO A., ZIMMERMAN H. M. Silver impregnation of nerve cells and fibers in celloidin sections. A simple impregnation technique. Arch Neurol. 1962 Feb;6:114–122. doi: 10.1001/archneur.1962.00450200028003. [DOI] [PubMed] [Google Scholar]
  8. Ishino H., Higashi S., Chûta M., Ohta H. Juvenile Alzheimer's disease with myoclonus: amyloid plaques and grumose alteration in the cerebellum. Clin Neuropathol. 1984 Sep-Oct;3(5):193–198. [PubMed] [Google Scholar]
  9. Kang J., Lemaire H. G., Unterbeck A., Salbaum J. M., Masters C. L., Grzeschik K. H., Multhaup G., Beyreuther K., Müller-Hill B. The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor. Nature. 1987 Feb 19;325(6106):733–736. doi: 10.1038/325733a0. [DOI] [PubMed] [Google Scholar]
  10. Kitamoto T., Ogomori K., Tateishi J., Prusiner S. B. Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids. Lab Invest. 1987 Aug;57(2):230–236. [PubMed] [Google Scholar]
  11. Kitamoto T., Tateishi J. Immunohistochemical confirmation of Creutzfeldt-Jakob disease with a long clinical course with amyloid plaque core antibodies. Am J Pathol. 1988 Jun;131(3):435–443. [PMC free article] [PubMed] [Google Scholar]
  12. Masters C. L., Simms G., Weinman N. A., Multhaup G., McDonald B. L., Beyreuther K. Amyloid plaque core protein in Alzheimer disease and Down syndrome. Proc Natl Acad Sci U S A. 1985 Jun;82(12):4245–4249. doi: 10.1073/pnas.82.12.4245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ogomori K., Kitamoto T., Tateishi J., Sato Y., Tashima T. Aging and cerebral amyloid: early detection of amyloid in the human brain using biochemical extraction and immunostain. J Gerontol. 1988 Nov;43(6):B157–B162. doi: 10.1093/geronj/43.6.b157. [DOI] [PubMed] [Google Scholar]
  14. Pro J. D., Smith C. H., Sumi S. M. Presenile Alzheimer disease: amyloid plaques in the cerebellum. Neurology. 1980 Aug;30(8):820–825. doi: 10.1212/wnl.30.8.820. [DOI] [PubMed] [Google Scholar]
  15. Probst A., Brunnschweiler H., Lautenschlager C., Ulrich J. A special type of senile plaque, possibly an initial stage. Acta Neuropathol. 1987;74(2):133–141. doi: 10.1007/BF00692843. [DOI] [PubMed] [Google Scholar]
  16. Robakis N. K., Ramakrishna N., Wolfe G., Wisniewski H. M. Molecular cloning and characterization of a cDNA encoding the cerebrovascular and the neuritic plaque amyloid peptides. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4190–4194. doi: 10.1073/pnas.84.12.4190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Roth M., Tomlinson B. E., Blessed G. Correlation between scores for dementia and counts of 'senile plaques' in cerebral grey matter of elderly subjects. Nature. 1966 Jan 1;209(5018):109–110. doi: 10.1038/209109a0. [DOI] [PubMed] [Google Scholar]
  18. SCHWARTZ P., KURUCZ J., KURUCZ A. RECENT OBSERVATIONS ON SENILE CEREBRAL CHANGES AND THEIR PATHOGENESIS. J Am Geriatr Soc. 1964 Oct;12:908–922. doi: 10.1111/j.1532-5415.1964.tb00640.x. [DOI] [PubMed] [Google Scholar]
  19. Tomlinson B. E., Blessed G., Roth M. Observations on the brains of demented old people. J Neurol Sci. 1970 Sep;11(3):205–242. doi: 10.1016/0022-510x(70)90063-8. [DOI] [PubMed] [Google Scholar]
  20. Tomlinson B. E., Blessed G., Roth M. Observations on the brains of non-demented old people. J Neurol Sci. 1968 Sep-Oct;7(2):331–356. doi: 10.1016/0022-510x(68)90154-8. [DOI] [PubMed] [Google Scholar]
  21. Vakili S. T., Muller J. Juvenile Alzheimer's disease with cerebellar involvement. Arch Pathol Lab Med. 1987 May;111(5):480–482. [PubMed] [Google Scholar]

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