Abstract
Polyclonal antibodies against osteonectin, a 32 kd non-collagenous bone protein, were applied for the histogenetic identification of variously differentiated osteosarcoma tissues. A strong positive reaction was found in matrix-producing osteosarcoma cells of the osteoblastic type, but pleomorphic or fibrosarcomatous osteosarcoma tissues reacted focally positive as well. Because the production of osteonectin depends on the osteoblastlike function of the individual tumor cell, a homogeneous immunocytochemical staining of all tumor cells cannot be expected. Nevertheless, the immunocytochemical demonstration of osteonectin in osteolytic tumors that produce no or scarcely any matrix seems to be a valuable tool for establishment of their osteogenic origin.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Church R. L. Chromosome mapping of connective tissue protein genes. Int Rev Connect Tissue Res. 1981;9:99–150. doi: 10.1016/B978-0-12-363709-3.50009-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
- Jundt G., Berghäuser K. H., Termine J. D., Schulz A. Osteonectin--a differentiation marker of bone cells. Cell Tissue Res. 1987 May;248(2):409–415. doi: 10.1007/BF00218209. [DOI] [PubMed] [Google Scholar]
- Martin S. E., Dwyer A., Kissane J. M., Costa J. Small-cell osteosarcoma. Cancer. 1982 Sep 1;50(5):990–996. doi: 10.1002/1097-0142(19820901)50:5<990::aid-cncr2820500529>3.0.co;2-r. [DOI] [PubMed] [Google Scholar]
- Price P. A., Otsuka A. A., Poser J. W., Kristaponis J., Raman N. Characterization of a gamma-carboxyglutamic acid-containing protein from bone. Proc Natl Acad Sci U S A. 1976 May;73(5):1447–1451. doi: 10.1073/pnas.73.5.1447. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Remberger K., Gay S. Immunhistochemical demonstration of different collagen types in the normal epiphyseal plate and in benign and malignant tumors of bone and cartilage. Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1977 Oct;90(1):95–106. doi: 10.1007/BF00306024. [DOI] [PubMed] [Google Scholar]
- Schulz A., Fischer H. P., Breithaupt H., Pralle H. Therapie-Response verschiedener histologischer Subtypen des Osteosarkoms unter hochdosierter Methotrexat-Behandlung. Onkologie. 1983 Dec;6(6):296–304. doi: 10.1159/000215252. [DOI] [PubMed] [Google Scholar]
- Sim F. H., Unni K. K., Beabout J. W., Dahlin D. C. Osteosarcoma with small cells simulating Ewing's tumor. J Bone Joint Surg Am. 1979 Mar;61(2):207–215. [PubMed] [Google Scholar]
- Straus W. Imidazole increases the sensitivity of the cytochemical reaction for peroxidase with diaminobenzidine at a neutral pH. J Histochem Cytochem. 1982 May;30(5):491–493. doi: 10.1177/30.5.6176617. [DOI] [PubMed] [Google Scholar]
- Termine J. D., Kleinman H. K., Whitson S. W., Conn K. M., McGarvey M. L., Martin G. R. Osteonectin, a bone-specific protein linking mineral to collagen. Cell. 1981 Oct;26(1 Pt 1):99–105. doi: 10.1016/0092-8674(81)90037-4. [DOI] [PubMed] [Google Scholar]
- von der Mark K. Localization of collagen types in tissues. Int Rev Connect Tissue Res. 1981;9:265–324. doi: 10.1016/b978-0-12-363709-3.50012-7. [DOI] [PubMed] [Google Scholar]