Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1988 Sep;132(3):512–520.

Bleomycin-stimulated hamster alveolar macrophages release interleukin-1.

A Suwabe 1, K Takahashi 1, S Yasui 1, S Arai 1, F Sendo 1
PMCID: PMC1880746  PMID: 2458043

Abstract

The capacity of alveolar macrophages (AM) of bleomycin-instilled hamsters to proliferate mouse thymocytes (interleukin-1 activity) and hamster fibroblasts (fibroblast proliferation (FP) activity was studied. Using bleomycin-instilled hamsters, the FP activity of AM culture supernatants was increased significantly on days 1, 5, and 10 after instillation of bleomycin. The interleukin-1 (IL-1) activity, however, was increased significantly on day 1 only as compared with saline-treated hamsters. Next, normal AM were stimulated in vitro by bleomycin. After being fractionated by chromatography, their culture supernatant showed IL-1 activity, which also indicated FP activity. These results suggest that bleomycin directly stimulates AM to release IL-1 in the fibrogenic responses.

Full text

PDF
512

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bigby T. D., Allen D., Leslie C. G., Henson P. M., Cherniack R. M. Bleomycin-induced lung injury in the rabbit. Analysis and correlation of bronchoalveolar lavage, morphometrics, and fibroblast stimulating activity. Am Rev Respir Dis. 1985 Sep;132(3):590–595. doi: 10.1164/arrd.1985.132.3.590. [DOI] [PubMed] [Google Scholar]
  2. Bitterman P. B., Rennard S. I., Hunninghake G. W., Crystal R. G. Human alveolar macrophage growth factor for fibroblasts. Regulation and partial characterization. J Clin Invest. 1982 Oct;70(4):806–822. doi: 10.1172/JCI110677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark J. G., Kostal K. M., Marino B. A. Bleomycin-induced pulmonary fibrosis in hamsters. An alveolar macrophage product increases fibroblast prostaglandin E2 and cyclic adenosine monophosphate and suppresses fibroblast proliferation and collagen production. J Clin Invest. 1983 Dec;72(6):2082–2091. doi: 10.1172/JCI111173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark J. G., Kuhn C., 3rd Bleomycin-induced pulmonary fibrosis in hamsters: effect of neutrophil depletion on lung collagen synthesis. Am Rev Respir Dis. 1982 Oct;126(4):737–739. doi: 10.1164/arrd.1982.126.4.737. [DOI] [PubMed] [Google Scholar]
  5. Conlon P. J., Henney C. S., Gillis S. Cytokine-dependent thymocyte responses: characterization of IL 1 and IL 2 target subpopulations and mechanism of action. J Immunol. 1982 Feb;128(2):797–801. [PubMed] [Google Scholar]
  6. Eden E., Turino G. M. Interleukin-1 secretion by human alveolar macrophages stimulated with endotoxin is augmented by recombinant immune (gamma) interferon. Am Rev Respir Dis. 1986 Mar;133(3):455–460. doi: 10.1164/arrd.1986.133.3.455. [DOI] [PubMed] [Google Scholar]
  7. Elias J. A., Rossman M. D., Zurier R. B., Daniele R. P. Human alveolar macrophage inhibition of lung fibroblast growth. A prostaglandin-dependent process. Am Rev Respir Dis. 1985 Jan;131(1):94–99. doi: 10.1164/arrd.1985.131.1.94. [DOI] [PubMed] [Google Scholar]
  8. Elias J. A., Zurier R. B., Rossman M. D., Berube M. L., Daniele R. P. Inhibition of lung fibroblast growth by human lung mononuclear cells. Am Rev Respir Dis. 1984 Nov;130(5):810–816. doi: 10.1164/arrd.1984.130.5.810. [DOI] [PubMed] [Google Scholar]
  9. Estes J. E., Pledger W. J., Gillespie G. Y. Macrophage-derived growth factor for fibroblasts and Interleukin-1 are distinct entities. J Leukoc Biol. 1984 Jan;35(1):115–129. doi: 10.1002/jlb.35.1.115. [DOI] [PubMed] [Google Scholar]
  10. Gritter H. L., Adamson I. Y., King G. M. Modulation of fibroblast activity by normal and silica-exposed alveolar macrophages. J Pathol. 1986 Apr;148(4):263–271. doi: 10.1002/path.1711480402. [DOI] [PubMed] [Google Scholar]
  11. Harington J. S., Ritchie M., King P. C., Miller K. The in-vitro effects of silica-treated hamster macrophages on collagen production by hamster fibroblasts. J Pathol. 1973 Jan;109(1):21–37. doi: 10.1002/path.1711090104. [DOI] [PubMed] [Google Scholar]
  12. Hunninghake G. W., Gadek J. E., Fales H. M., Crystal R. G. Human alveolar macrophage-derived chemotactic factor for neutrophils. Stimuli and partial characterization. J Clin Invest. 1980 Sep;66(3):473–483. doi: 10.1172/JCI109878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hunninghake G. W. Release of interleukin-1 by alveolar macrophages of patients with active pulmonary sarcoidosis. Am Rev Respir Dis. 1984 Apr;129(4):569–572. [PubMed] [Google Scholar]
  14. Kaelin R. M., Center D. M., Bernardo J., Grant M., Snider G. L. The role of macrophage-derived chemoattractant activities in the early inflammatory events of bleomycin-induced pulmonary injury. Am Rev Respir Dis. 1983 Jul;128(1):132–137. doi: 10.1164/arrd.1983.128.1.132. [DOI] [PubMed] [Google Scholar]
  15. Koretzky G. A., Elias J. A., Kay S. L., Rossman M. D., Nowell P. C., Daniele R. P. Spontaneous production of interleukin-1 by human alveolar macrophages. Clin Immunol Immunopathol. 1983 Dec;29(3):443–450. doi: 10.1016/0090-1229(83)90047-8. [DOI] [PubMed] [Google Scholar]
  16. Kovacs E. J., Kelley J. Intra-alveolar release of a competence-type growth factor after lung injury. Am Rev Respir Dis. 1986 Jan;133(1):68–72. doi: 10.1164/arrd.1986.133.1.68. [DOI] [PubMed] [Google Scholar]
  17. Kovacs E. J., Kelley J. Intra-alveolar release of a competence-type growth factor after lung injury. Am Rev Respir Dis. 1986 Jan;133(1):68–72. doi: 10.1164/arrd.1986.133.1.68. [DOI] [PubMed] [Google Scholar]
  18. Kovacs E. J., Kelley J. Secretion of macrophage-derived growth factor during acute lung injury induced by bleomycin. J Leukoc Biol. 1985 Jan;37(1):1–14. doi: 10.1002/jlb.37.1.1. [DOI] [PubMed] [Google Scholar]
  19. Lamontagne L., Gauldie J., Stadnyk A., Richards C., Jenkins E. In vivo initiation of unstimulated in vitro interleukin-1 release by alveolar macrophages. Am Rev Respir Dis. 1985 Mar;131(3):326–330. doi: 10.1164/arrd.1985.131.3.326. [DOI] [PubMed] [Google Scholar]
  20. Leibovich S. J., Ross R. A macrophage-dependent factor that stimulates the proliferation of fibroblasts in vitro. Am J Pathol. 1976 Sep;84(3):501–514. [PMC free article] [PubMed] [Google Scholar]
  21. Lugano E. M., Dauber J. H., Elias J. A., Bashey R. I., Jimenez S. A., Daniele R. P. The regulation of lung fibroblast proliferation by alveolar macrophages in experimental silicosis. Am Rev Respir Dis. 1984 May;129(5):767–771. doi: 10.1164/arrd.1984.129.5.767. [DOI] [PubMed] [Google Scholar]
  22. MYRVIK Q., LEAKE E. S., FARISS B. Studies on pulmonary alveolar macrophages from the normal rabbit: a technique to procure them in a high state of purity. J Immunol. 1961 Feb;86:128–132. [PubMed] [Google Scholar]
  23. Phan S. H., Kunkel S. L. Inhibition of bleomycin-induced pulmonary fibrosis by nordihydroguaiaretic acid. The role of alveolar macrophage activation and mediator production. Am J Pathol. 1986 Aug;124(2):343–352. [PMC free article] [PubMed] [Google Scholar]
  24. Phan S. H., Thrall R. S. The role of soluble factors in bleomycin-induced pulmonary fibrosis. Am J Pathol. 1982 Feb;106(2):156–164. [PMC free article] [PubMed] [Google Scholar]
  25. Reisner Y., Linker-Israeli M., Sharon N. Separation of mouse thymocytes into two subpopulations by the use of peanut agglutinin. Cell Immunol. 1976 Jul;25(1):129–134. doi: 10.1016/0008-8749(76)90103-9. [DOI] [PubMed] [Google Scholar]
  26. Reynolds H. Y., Newball H. H. Analysis of proteins and respiratory cells obtained from human lungs by bronchial lavage. J Lab Clin Med. 1974 Oct;84(4):559–573. [PubMed] [Google Scholar]
  27. Ross R., Nist C., Kariya B., Rivest M. J., Raines E., Callis J. Physiological quiescence in plasma-derived serum: influence of platelet-derived growth factor on cell growth in culture. J Cell Physiol. 1978 Dec;97(3 Pt 2 Suppl 1):497–508. doi: 10.1002/jcp.1040970325. [DOI] [PubMed] [Google Scholar]
  28. Schmidt J. A., Mizel S. B., Cohen D., Green I. Interleukin 1, a potential regulator of fibroblast proliferation. J Immunol. 1982 May;128(5):2177–2182. [PubMed] [Google Scholar]
  29. Snider G. L., Celli B. R., Goldstein R. H., O'Brien J. J., Lucey E. C. Chronic interstitial pulmonary fibrosis produced in hamsters by endotracheal bleomycin. Lung volumes, volume-pressure relations, carbon monoxide uptake, and arterial blood gas studied. Am Rev Respir Dis. 1978 Feb;117(2):289–297. doi: 10.1164/arrd.1978.117.2.289. [DOI] [PubMed] [Google Scholar]
  30. Snider G. L., Hayes J. A., Korthy A. L. Chronic interstitial pulmonary fibrosis produced in hamsters by endotracheal bleomycin: pathology and stereology. Am Rev Respir Dis. 1978 Jun;117(6):1099–1108. doi: 10.1164/arrd.1978.117.6.1099. [DOI] [PubMed] [Google Scholar]
  31. Thrall R. S., Barton R. W., D'Amato D. A., Sulavik S. B. Differential cellular analysis of bronchoalveolar lavage fluid obtained at various stages during the development of bleomycin-induced pulmonary fibrosis in the rat. Am Rev Respir Dis. 1982 Sep;126(3):488–492. doi: 10.1164/arrd.1982.126.3.488. [DOI] [PubMed] [Google Scholar]
  32. Thrall R. S., Phan S. H., McCormick J. R., Ward P. A. The development of bleomycin-induced pulmonary fibrosis in neutrophil-depleted and complement-depleted rats. Am J Pathol. 1981 Oct;105(1):76–81. [PMC free article] [PubMed] [Google Scholar]
  33. Wesselius L. J., Catanzaro A., Wasserman S. I. Neutrophil chemotactic activity generation by alveolar macrophages after bleomycin injury. Am Rev Respir Dis. 1984 Mar;129(3):485–490. doi: 10.1164/arrd.1984.129.3.485. [DOI] [PubMed] [Google Scholar]
  34. Wewers M. D., Rennard S. I., Hance A. J., Bitterman P. B., Crystal R. G. Normal human alveolar macrophages obtained by bronchoalveolar lavage have a limited capacity to release interleukin-1. J Clin Invest. 1984 Dec;74(6):2208–2218. doi: 10.1172/JCI111647. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES