Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1988 Dec;133(3):623–629.

Neutrophil mobilization induced by complement fragments during experimental group B streptococcal (GBS) infection.

A O Shigeoka 1, R J Gobel 1, J Janatova 1, H R Hill 1
PMCID: PMC1880801  PMID: 3059808

Abstract

Degradation products of the third component of complement have been reported to have the ability to mobilize leukocytes from the marrow and induce leukocytosis. The effect of C3d,g preparations on neutrophil responses in a neonatal rat model of group B streptococcal infection in which neutrophil mobilization from the marrow is inadequate has been evaluated. Dimeric and monomeric fragments of C3d,g were isolated from human serum; the identity of the C3d,g preparations was confirmed by SDS-PAGE, Western blotting, and N-terminal amino acid sequencing. Uninfected neonatal rats responded to intraperitoneal injection of C3d,g with a peripheral blood neutrophilia at 30 minutes and 4 hours after inoculation. C3d,g, which lacks intrinsic chemotactic activity, enhanced the local accumulation of neutrophils in the peritoneal cavity of infected, but not uninfected, neonatal rats. In addition, myeloid cell release from the marrow of isolated femurs of neonatal rats receiving C3d,g was significantly enhanced. Thus, the effect of C3d,g in this model was to mobilize marrow cells and induce peripheral leukocytosis. Chemotactic factors released at the site of infection then resulted in the local accumulation of these inflammatory cells. Complement-derived components capable of releasing marrow myeloid elements may play a major role in determining the outcome of bacterial infection in the immature host.

Full text

PDF
623

Images in this article

625Figure 1
on p.625

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chervenick P. A., Boggs D. R., Marsh J. C., Cartwright G. E., Wintrobe M. M. Quantitative studies of blood and bone marrow neutrophils in normal mice. Am J Physiol. 1968 Aug;215(2):353–360. doi: 10.1152/ajplegacy.1968.215.2.353. [DOI] [PubMed] [Google Scholar]
  2. Christensen R. D., Rothstein G., Hill H. R., Pincus S. H. The effect of hybridoma antibody administration upon neutrophil kinetics during experimental type III group B streptococcal sepsis. Pediatr Res. 1983 Oct;17(10):795–799. doi: 10.1203/00006450-198310000-00005. [DOI] [PubMed] [Google Scholar]
  3. Christensen R. D., Shigeoka A. O., Hill H. R., Rothstein G. Circulating and storage neutrophil changes in experimental type II group B streptococcal sepsis. Pediatr Res. 1980 Jun;14(6):806–808. doi: 10.1203/00006450-198006000-00006. [DOI] [PubMed] [Google Scholar]
  4. Cross A. S., Wooldridge W. H., Zollinger W. D. Monoclonal antibody 2-2-B kills K1-positive Escherichia coli in conjunction with cord blood neutrophils and sera, but not with spinal fluid. Pediatr Res. 1984 Aug;18(8):770–772. doi: 10.1203/00006450-198408000-00021. [DOI] [PubMed] [Google Scholar]
  5. Davis A. E., 3rd, Harrison R. A., Lachmann P. J. Physiologic inactivation of fluid phase C3b: isolation and structural analysis of C3c, C3d,g (alpha 2D), and C3g. J Immunol. 1984 Apr;132(4):1960–1966. [PubMed] [Google Scholar]
  6. Falk W., Goodwin R. H., Jr, Leonard E. J. A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration. J Immunol Methods. 1980;33(3):239–247. doi: 10.1016/0022-1759(80)90211-2. [DOI] [PubMed] [Google Scholar]
  7. Fireman P., Zuchowski D. A., Taylor P. M. Development of human complement system. J Immunol. 1969 Jul;103(1):25–31. [PubMed] [Google Scholar]
  8. Gaither T. A., Hammer C. H., Gadek J. E., Katusha K., Santaella M., Frank M. M. Cleavage of membrane-bound C3b and C3bi by viable human neutrophils (PMN). Mol Immunol. 1983 Jun;20(6):623–635. doi: 10.1016/0161-5890(83)90007-x. [DOI] [PubMed] [Google Scholar]
  9. Ghebrehiwet B., Müller-Eberhard H. J. C3e: an acidic fragment of human C3 with leukocytosis-inducing activity. J Immunol. 1979 Aug;123(2):616–621. [PubMed] [Google Scholar]
  10. Harvath L., Falk W., Leonard E. J. Rapid quantitation of neutrophil chemotaxis: use of a polyvinylpyrrolidone-free polycarbonate membrane in a multiwell assembly. J Immunol Methods. 1980;37(1):39–45. doi: 10.1016/0022-1759(80)90179-9. [DOI] [PubMed] [Google Scholar]
  11. Hoeprich P. D., Jr, Dahinden C. A., Lachmann P. J., Davis A. E., 3rd, Hugli T. E. A synthetic nonapeptide corresponding to the NH2-terminal sequence of C3d-K causes leukocytosis in rabbits. J Biol Chem. 1985 Mar 10;260(5):2597–2600. [PubMed] [Google Scholar]
  12. Hostetter M. K. Serotypic variations among virulent pneumococci in deposition and degradation of covalently bound C3b: implications for phagocytosis and antibody production. J Infect Dis. 1986 Apr;153(4):682–693. doi: 10.1093/infdis/153.4.682. [DOI] [PubMed] [Google Scholar]
  13. Iida K., Nadler L., Nussenzweig V. Identification of the membrane receptor for the complement fragment C3d by means of a monoclonal antibody. J Exp Med. 1983 Oct 1;158(4):1021–1033. doi: 10.1084/jem.158.4.1021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Janatova J., Gobel R. J. Activation and fragmentation of the third (C3) and the fourth (C4) components of complement: generation and isolation of physiologically relevant fragments C3c and C4c. J Immunol Methods. 1985 Dec 17;85(1):17–26. doi: 10.1016/0022-1759(85)90270-4. [DOI] [PubMed] [Google Scholar]
  15. Janatova J., Gobel R. J. Rapid optimization of immunoadsorbent characteristics. Biochem J. 1984 Jul 1;221(1):113–120. doi: 10.1042/bj2210113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  17. Meuth J. L., Morgan E. L., DiSipio R. G., Hugli T. E. Suppression of T lymphocyte functions by human C3 fragments. I. Inhibition of human T cell proliferative responses by a kallikrein cleavage fragment of human iC3b. J Immunol. 1983 Jun;130(6):2605–2611. [PubMed] [Google Scholar]
  18. Mills E. L., Björksteń B., Quie P. G. Deficient alternative complement pathway activity in newborn sera. Pediatr Res. 1979 Dec;13(12):1341–1344. doi: 10.1203/00006450-197912000-00008. [DOI] [PubMed] [Google Scholar]
  19. Rother K. Leucocyte mobilizing factor: a new biological activity derived from the third component of complement. Eur J Immunol. 1972 Dec;2(6):550–558. doi: 10.1002/eji.1830020615. [DOI] [PubMed] [Google Scholar]
  20. Seya T., Nagasawa S. Limited proteolysis of complement protein C3b by regulatory enzyme C3b inactivator: isolation and characterization of a biologically active fragment, C3d,g. J Biochem. 1985 Jan;97(1):373–382. doi: 10.1093/oxfordjournals.jbchem.a135064. [DOI] [PubMed] [Google Scholar]
  21. Shigeoka A. O., Jensen C. L., Pincus S. H., Hill H. R. Absolute requirement for complement in monoclonal IgM antibody-mediated protection against experimental infection with type III group B streptococci. J Infect Dis. 1984 Jul;150(1):63–70. doi: 10.1093/infdis/150.1.63. [DOI] [PubMed] [Google Scholar]
  22. Shigeoka A. O., Pincus S. H., Rote N. S., Hill H. R. Protective efficacy of hybridoma type-specific antibody against experimental infection with group-B Streptococcus. J Infect Dis. 1984 Mar;149(3):363–372. doi: 10.1093/infdis/149.3.363. [DOI] [PubMed] [Google Scholar]
  23. Shigeoka A. O., Rote N. S., Santos J. I., Hill H. R. Assessment of the virulence factors of group B streptococci: correlation with sialic acid content. J Infect Dis. 1983 May;147(5):857–863. doi: 10.1093/infdis/147.5.857. [DOI] [PubMed] [Google Scholar]
  24. Shigeoka A. O., Weber M. E., Pincus S. H., Pritchard D. G., Egan M. L., Hill H. R. Type-specific monoclonal antibody enhances the local phagocytic response to group B streptococcal infection. J Infect Dis. 1986 Jun;153(6):1170–1173. doi: 10.1093/infdis/153.6.1170. [DOI] [PubMed] [Google Scholar]
  25. Steinbuch M., Audran R. The isolation of IgG from mammalian sera with the aid of caprylic acid. Arch Biochem Biophys. 1969 Nov;134(2):279–284. doi: 10.1016/0003-9861(69)90285-9. [DOI] [PubMed] [Google Scholar]
  26. Vik D. P., Fearon D. T. Neutrophils express a receptor for iC3b, C3dg, and C3d that is distinct from CR1, CR2, and CR3. J Immunol. 1985 Apr;134(4):2571–2579. [PubMed] [Google Scholar]
  27. WARD P. A., COCHRANE C. G., MUELLER-EBERHARD H. J. THE ROLE OF SERUM COMPLEMENT IN CHEMOTAXIS OF LEUKOCYTES IN VITRO. J Exp Med. 1965 Aug 1;122:327–346. doi: 10.1084/jem.122.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES