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. 1988 Dec;133(3):615–622.

Degree of malignancy of thymic epithelial tumors in terms of nuclear DNA content and nuclear area. An analysis of 39 cases.

H Asamura 1, T Nakajima 1, K Mukai 1, M Noguchi 1, Y Shimosato 1
PMCID: PMC1880827  PMID: 3202120

Abstract

For determination of the degree of malignancy among thymic epithelial tumors, the DNA content and area of nuclei in 13 cases each of noninvasive thymoma, invasive thymoma, and thymic carcinoma were investigated by cytofluorometry and morphometry. The nuclear DNA content was determined in terms of the mean nuclear DNA content, DNA histogram pattern, and the occurrence of the aneuploid stem cell line. The mean nuclear DNA content of the thymic carcinoma was significantly higher than that of both subgroups of thymoma (P less than 0.01), but there was no significant difference between noninvasive and invasive thymomas. The aneuploid stem cell line appeared in 92.3% of thymic carcinomas, one case (7.7%) of invasive thymomas, and none of noninvasive thymomas. Abnormal DNA histogram patterns were seen in 53.8% of thymic carcinomas and none of the thymomas. The mean nuclear area increased significantly in the increasing order of noninvasive thymoma, invasive thymoma, and thymic carcinoma (P less than 0.01). The cytofluorometric and morphometric results demonstrated a significant difference in degree of malignancy between thymic carcinoma and thymoma; however, there was a trend toward an increasing degree of malignancy from noninvasive to invasive thymomas, yet there was a sizeable overlap in results between the two groups. Therefore, these two methods are not satisfactory for predicting the behavior of an individual case of noninvasive or invasive thymoma.

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Selected References

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  1. Atkin N. B., Kay R. Prognostic significance of modal DNA value and other factors in malignant tumours, based on 1465 cases. Br J Cancer. 1979 Aug;40(2):210–221. doi: 10.1038/bjc.1979.168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barlogie B., Drewinko B., Schumann J., Göhde W., Dosik G., Latreille J., Johnston D. A., Freireich E. J. Cellular DNA content as a marker of neoplasia in man. Am J Med. 1980 Aug;69(2):195–203. doi: 10.1016/0002-9343(80)90379-4. [DOI] [PubMed] [Google Scholar]
  3. Blöndal T., Bengtsson A. Nuclear DNA measurements in squamous cell carcinoma of the lung: a guide for prognostic evaluation. Anticancer Res. 1981;1(2):79–86. [PubMed] [Google Scholar]
  4. Blöndal T., Lindgren A. Fluorescence cytophotometric measurements of nuclear DNA in adenocarcinoma of the lung; relation of proliferative activity and DNA ploidy to prognosis. Anal Quant Cytol. 1982 Sep;4(3):225–232. [PubMed] [Google Scholar]
  5. Bunn P. A., Jr, Carney D. N., Gazdar A. F., Whang-Peng J., Matthews M. J. Diagnostic and biological implications of flow cytometric DNA content analysis in lung cancer. Cancer Res. 1983 Oct;43(10):5026–5032. [PubMed] [Google Scholar]
  6. Emdin S. O., Stenling R., Roos G. Prognostic value of DNA content in colorectal carcinoma. A flow cytometric study with some methodologic aspects. Cancer. 1987 Sep 15;60(6):1282–1287. doi: 10.1002/1097-0142(19870915)60:6<1282::aid-cncr2820600620>3.0.co;2-f. [DOI] [PubMed] [Google Scholar]
  7. Ewers S. B., Långström E., Baldetorp B., Killander D. Flow-cytometric DNA analysis in primary breast carcinomas and clinicopathological correlations. Cytometry. 1984 Jul;5(4):408–419. doi: 10.1002/cyto.990050419. [DOI] [PubMed] [Google Scholar]
  8. Frankfurt O. S., Slocum H. K., Rustum Y. M., Arbuck S. G., Pavelic Z. P., Petrelli N., Huben R. P., Pontes E. J., Greco W. R. Flow cytometric analysis of DNA aneuploidy in primary and metastatic human solid tumors. Cytometry. 1984 Jan;5(1):71–80. doi: 10.1002/cyto.990050111. [DOI] [PubMed] [Google Scholar]
  9. Fried J., Perez A. G., Clarkson B. D. Flow cytofluorometric analysis of cell cycle distributions using propidium iodide. Properties of the method and mathematical analysis of the data. J Cell Biol. 1976 Oct;71(1):172–181. doi: 10.1083/jcb.71.1.172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Friedlander M. L., Hedley D. W., Taylor I. W., Russell P., Coates A. S., Tattersall M. H. Influence of cellular DNA content on survival in advanced ovarian cancer. Cancer Res. 1984 Jan;44(1):397–400. [PubMed] [Google Scholar]
  11. Gerein A. N., Srivastava S. P., Burgess J. Thymoma: a ten year review. Am J Surg. 1978 Jul;136(1):49–53. doi: 10.1016/0002-9610(78)90199-x. [DOI] [PubMed] [Google Scholar]
  12. Gray G. F., Gutowski W. T., 3rd Thymoma. A clinicopathologic study of 54 cases. Am J Surg Pathol. 1979 Jun;3(3):235–249. [PubMed] [Google Scholar]
  13. Hattori T., Hosokawa Y., Fukuda M., Sugihara H., Hamada S., Takamatsu T., Nakanishi K., Tsuchihashi Y., Kitamura T., Fujita S. Analysis of DNA ploidy patterns of gastric carcinomas of Japanese. Cancer. 1984 Oct 15;54(8):1591–1597. doi: 10.1002/1097-0142(19841015)54:8<1591::aid-cncr2820540821>3.0.co;2-k. [DOI] [PubMed] [Google Scholar]
  14. Hedley D. W., Friedlander M. L., Taylor I. W., Rugg C. A., Musgrove E. A. Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem. 1983 Nov;31(11):1333–1335. doi: 10.1177/31.11.6619538. [DOI] [PubMed] [Google Scholar]
  15. Joensuu H., Söderström K. O., Klemi P. J., Eerola E. Nuclear DNA content and its prognostic value in lymphoma of the stomach. Cancer. 1987 Dec 15;60(12):3042–3048. doi: 10.1002/1097-0142(19871215)60:12<3042::aid-cncr2820601231>3.0.co;2-9. [DOI] [PubMed] [Google Scholar]
  16. Kodama T., Watanabe S., Sato Y., Shimosato Y., Miyazawa N. An immunohistochemical study of thymic epithelial tumors. I. Epithelial component. Am J Surg Pathol. 1986 Jan;10(1):26–33. doi: 10.1097/00000478-198601000-00004. [DOI] [PubMed] [Google Scholar]
  17. Levine G. D., Rosai J. Thymic hyperplasia and neoplasia: a review of current concepts. Hum Pathol. 1978 Sep;9(5):495–515. doi: 10.1016/s0046-8177(78)80131-2. [DOI] [PubMed] [Google Scholar]
  18. Lewis J. E., Wick M. R., Scheithauer B. W., Bernatz P. E., Taylor W. F. Thymoma. A clinicopathologic review. Cancer. 1987 Dec 1;60(11):2727–2743. doi: 10.1002/1097-0142(19871201)60:11<2727::aid-cncr2820601125>3.0.co;2-d. [DOI] [PubMed] [Google Scholar]
  19. Maggi G., Giaccone G., Donadio M., Ciuffreda L., Dalesio O., Leria G., Trifiletti G., Casadio C., Palestro G., Mancuso M. Thymomas. A review of 169 cases, with particular reference to results of surgical treatment. Cancer. 1986 Aug 1;58(3):765–776. doi: 10.1002/1097-0142(19860801)58:3<765::aid-cncr2820580326>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  20. Morinaga S., Sato Y., Shimosato Y., Sinkai T., Tsuchiya R. Multiple thymic squamous cell carcinomas associated with mixed type thymoma. Am J Surg Pathol. 1987 Dec;11(12):982–988. doi: 10.1097/00000478-198712000-00009. [DOI] [PubMed] [Google Scholar]
  21. Nomori H., Horinouchi H., Kaseda S., Ishihara T., Torikata C. Evaluation of the malignant grade of thymoma by morphometric analysis. Cancer. 1988 Mar 1;61(5):982–988. doi: 10.1002/1097-0142(19880301)61:5<982::aid-cncr2820610521>3.0.co;2-1. [DOI] [PubMed] [Google Scholar]
  22. Nomori H., Shimosato Y., Kodama T., Morinaga S., Nakajima T., Watanabe S. Subtypes of small cell carcinoma of the lung: morphometric, ultrastructural, and immunohistochemical analyses. Hum Pathol. 1986 Jun;17(6):604–613. doi: 10.1016/s0046-8177(86)80133-2. [DOI] [PubMed] [Google Scholar]
  23. Rosenow E. C., 3rd, Hurley B. T. Disorders of the thymus. A review. Arch Intern Med. 1984 Apr;144(4):763–770. [PubMed] [Google Scholar]
  24. Sato Y., Watanabe S., Mukai K., Kodama T., Upton M. P., Goto M., Shimosato Y. An immunohistochemical study of thymic epithelial tumors. II. Lymphoid component. Am J Surg Pathol. 1986 Dec;10(12):862–870. doi: 10.1097/00000478-198612000-00005. [DOI] [PubMed] [Google Scholar]
  25. Shimosato Y., Kameya T., Nagai K., Suemasu K. Squamous cell carcinoma of the thymus. An analysis of eight cases. Am J Surg Pathol. 1977 Jun;1(2):109–121. doi: 10.1097/00000478-197706000-00002. [DOI] [PubMed] [Google Scholar]
  26. Shinkai T., Saijo N., Yoshioka S., Suemasu K., Hasegawa H., Miyazawa N., Kamiya T., Kanzawa F., Nakajima T., Shimosato Y. Recurrence of thymoma with appearance of myasthenia gravis 18 years after surgery: a case report. Jpn J Clin Oncol. 1985 Sep;15(3):567–575. [PubMed] [Google Scholar]
  27. Snover D. C., Levine G. D., Rosai J. Thymic carcinoma. Five distinctive histological variants. Am J Surg Pathol. 1982 Jul;6(5):451–470. [PubMed] [Google Scholar]
  28. Teodori L., Tirindelli-Danesi D., Mauro F., De Vita R., Uccelli R., Botti C., Modini C., Nervi C., Stipa S. Non-small-cell lung carcinoma: tumor characterization on the basis of flow cytometrically determined cellular heterogeneity. Cytometry. 1983 Sep;4(2):174–183. doi: 10.1002/cyto.990040212. [DOI] [PubMed] [Google Scholar]
  29. Tirindelli-Danesi D., Teodori L., Mauro F., Modini C., Botti C., Cicconetti F., Stipa S. Prognostic significance of flow cytometry in lung cancer. A 5-year study. Cancer. 1987 Aug 15;60(4):844–851. doi: 10.1002/1097-0142(19870815)60:4<844::aid-cncr2820600421>3.0.co;2-3. [DOI] [PubMed] [Google Scholar]
  30. Verley J. M., Hollmann K. H. Thymoma. A comparative study of clinical stages, histologic features, and survival in 200 cases. Cancer. 1985 Mar 1;55(5):1074–1086. doi: 10.1002/1097-0142(19850301)55:5<1074::aid-cncr2820550524>3.0.co;2-t. [DOI] [PubMed] [Google Scholar]
  31. Vindeløv L. L., Hansen H. H., Christensen I. J., Spang-Thomsen M., Hirsch F. R., Hansen M., Nissen N. I. Clonal heterogeneity of small-cell anaplastic carcinoma of the lung demonstrated by flow-cytometric DNA analysis. Cancer Res. 1980 Nov;40(11):4295–4300. [PubMed] [Google Scholar]
  32. Volm M., Drings P., Mattern J., Sonka J., Vogt-Moykopf I., Wayss K. Prognostic significance of DNA patterns and resistance-predictive tests in non-small cell lung carcinoma. Cancer. 1985 Sep 15;56(6):1396–1403. doi: 10.1002/1097-0142(19850915)56:6<1396::aid-cncr2820560630>3.0.co;2-l. [DOI] [PubMed] [Google Scholar]
  33. Volm M., Mattern J., Sonka J., Vogt-Schaden M., Wayss K. DNA distribution in non-small-cell lung carcinomas and its relationship to clinical behavior. Cytometry. 1985 Jul;6(4):348–356. doi: 10.1002/cyto.990060412. [DOI] [PubMed] [Google Scholar]
  34. Wick M. R., Scheithauer B. W., Weiland L. H., Bernatz P. E. Primary thymic carcinomas. Am J Surg Pathol. 1982 Oct;6(7):613–630. doi: 10.1097/00000478-198210000-00003. [DOI] [PubMed] [Google Scholar]
  35. Wolley R. C., Schreiber K., Koss L. G., Karas M., Sherman A. DNA distribution in human colon carcinomas and its relationship to clinical behavior. J Natl Cancer Inst. 1982 Jul;69(1):15–22. [PubMed] [Google Scholar]

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