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. 1993 Sep;37(9):1962–1965. doi: 10.1128/aac.37.9.1962

Fluconazole resistance in Candida glabrata.

C A Hitchcock 1, G W Pye 1, P F Troke 1, E M Johnson 1, D W Warnock 1
PMCID: PMC188100  PMID: 8239613

Abstract

We report a case of infection with Candida glabrata in which the organism became resistant to fluconazole and in which pre- and posttreatment isolates were available for comparison. The organism was cross-resistant to ketoconazole and itraconazole, in common with other azole-resistant yeasts. Fluconazole was a potent inhibitor of cytochrome P-450-dependent 14 alpha-sterol demethylase (P-450DM) in lysates of cells from both susceptible and resistant cultures (50% inhibitory concentration, 0.2 microM), indicating that resistance was unrelated to changes in P-450DM. Instead, it appeared to arise from a permeability barrier to fluconazole, since resistant cells were unable to take up radiolabelled drug.

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Selected References

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