Abstract
Susceptibility testing was performed on Dientamoeba fragilis ATCC 30948 in a dixenic culture with Klebsiella pneumoniae and Bacteroides vulgatus. D. fragilis was cocultured with the bacteria in TYGM-9 medium (ATCC medium 1171). The activities of antiparasitic drugs were assessed by counting viable D. fragilis trophozoites with a hemacytometer by trypan blue exclusion. The minimal amebicidal concentrations of the following four drugs were determined: iodoquinol at 128 micrograms/ml, paromomycin at 16 micrograms/ml, tetracycline (questionably) at 32 micrograms/ml, and metronidazole at 32 micrograms/ml.
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Selected References
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- BALAMUTH W. Comparative action of selected amebicidal agents and antibiotics against several species of human intestinal amebae. Am J Trop Med Hyg. 1953 Mar;2(2):191–205. doi: 10.4269/ajtmh.1953.2.191. [DOI] [PubMed] [Google Scholar]
- BURROWS R. B., SWERDLOW M. A. Enterobius vermicularis as a probable vector of Dientamoeba fragilis. Am J Trop Med Hyg. 1956 Mar;5(2):258–265. doi: 10.4269/ajtmh.1956.5.258. [DOI] [PubMed] [Google Scholar]
- Bolton R. P., Culshaw M. A. Faecal metronidazole concentrations during oral and intravenous therapy for antibiotic associated colitis due to Clostridium difficile. Gut. 1986 Oct;27(10):1169–1172. doi: 10.1136/gut.27.10.1169. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bruckner D. A., Garcia L. S., Voge M. Intestinal parasites in Los Angeles, California. Am J Med Technol. 1979 Dec;45(12):1020–1022. [PubMed] [Google Scholar]
- Chan F. T., Guan M. X., Mackenzie A. M. Application of indirect immunofluorescence to detection of Dientamoeba fragilis trophozoites in fecal specimens. J Clin Microbiol. 1993 Jul;31(7):1710–1714. doi: 10.1128/jcm.31.7.1710-1714.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Diamond L. S., Harlow D. R., Cunnick C. C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg. 1978;72(4):431–432. doi: 10.1016/0035-9203(78)90144-x. [DOI] [PubMed] [Google Scholar]
- Diamond L. S. Improved method for the monoxenic cultivation of Entamoeba histolytica Schaudinn, 1903 and E. histolytica-like amebae with trypanosomatids. J Parasitol. 1968 Aug;54(4):715–719. [PubMed] [Google Scholar]
- Gillin F. D., Diamond L. S. Clonal growth of Entamoeba histolytica and other species of Entamoeba in agar. J Protozool. 1978 Nov;25(4):539–543. doi: 10.1111/j.1550-7408.1978.tb04182.x. [DOI] [PubMed] [Google Scholar]
- Johnson P. J. Metronidazole and drug resistance. Parasitol Today. 1993 May;9(5):183–186. doi: 10.1016/0169-4758(93)90143-4. [DOI] [PubMed] [Google Scholar]
- Kean B. H., Malloch C. L. The neglected ameba: dientamoeba fragilis. A report of 100 "pure" infections. Am J Dig Dis. 1966 Sep;11(9):735–746. doi: 10.1007/BF02239427. [DOI] [PubMed] [Google Scholar]
- Levine N. D., Corliss J. O., Cox F. E., Deroux G., Grain J., Honigberg B. M., Leedale G. F., Loeblich A. R., 3rd, Lom J., Lynn D. A newly revised classification of the protozoa. J Protozool. 1980 Feb;27(1):37–58. doi: 10.1111/j.1550-7408.1980.tb04228.x. [DOI] [PubMed] [Google Scholar]
- Millet V., Spencer M. J., Chapin M., Stewart M., Yatabe J. A., Brewer T., Garcia L. S. Dientamoeba fragilis, a protozoan parasite in adult members of a semicommunal group. Dig Dis Sci. 1983 Apr;28(4):335–339. doi: 10.1007/BF01324950. [DOI] [PubMed] [Google Scholar]
- Spencer M. J., Chapin M. R., Garcia L. S. Dientamoeba fragilis: a gastrointestinal protozoan infection in adults. Am J Gastroenterol. 1982 Aug;77(8):565–569. [PubMed] [Google Scholar]
- Spencer M. J., Garcia L. S., Chapin M. R. Dientamoeba fragilis. An intestinal pathogen in children? Am J Dis Child. 1979 Apr;133(4):390–393. [PubMed] [Google Scholar]
- Steinitz H., Talis B., Stein B. Entamoeba histolytica and Dientamoeba fragilis and the syndrome of chronic recurrent intestinal amoebiasis in Israel. Digestion. 1970;3(3):146–153. doi: 10.1159/000197025. [DOI] [PubMed] [Google Scholar]
- Talis B., Stein B., Lengy J. Dientamoeba fragilis in human feces and bile. Isr J Med Sci. 1971 Sep;7(9):1063–1069. [PubMed] [Google Scholar]
- Yang J., Scholten T. Dientamoeba fragilis: a review with notes on its epidemiology, pathogenicity, mode of transmission, and diagnosis. Am J Trop Med Hyg. 1977 Jan;26(1):16–22. doi: 10.4269/ajtmh.1977.26.16. [DOI] [PubMed] [Google Scholar]
- Zierdt C. H. Blastocystis hominis--past and future. Clin Microbiol Rev. 1991 Jan;4(1):61–79. doi: 10.1128/cmr.4.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
