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. 1991 Jul;139(1):161–168.

Rearrangement of the T-cell receptor delta chain gene in T-cell lymphomas with a mature phenotype.

J H van Krieken 1, L Elwood 1, R E Andrade 1, E S Jaffe 1, J Cossman 1, L J Medeiros 1
PMCID: PMC1886140  PMID: 1830192

Abstract

The configuration of the T-cell receptor (TCR) delta chain gene was assessed using restriction fragment analysis and the Southern blot technique in 39 T-cell lymphomas with a mature immunophenotype. The TCR delta gene was rearranged in four lymphomas although the gamma/delta TCR was not expressed in two cases studied. The TCR delta gene was the only TCR gene rearranged in two cases. Each lymphoma with TCR delta gene rearrangement had an aberrant T-cell immunophenotype and three cases were of the large cell anaplastic type. The TCR delta gene was deleted in 22 cases and was in the germline configuration in 13 lymphomas. Deletion of the TCR delta gene was characteristic of mycosis fungoides, adult T-cell leukemia/lymphoma (human T cell leukemia-lymphoma virus positive), and Lennert's lymphoma, and was not identified in angiocentric lymphomas. In eight cases with TCR delta deletion, however, a large number of polyclonal (presumably reactive) T cells were present and, in these lymphomas, the authors could not determine if TCR delta gene deletion occurred in the polyclonal T cells, the neoplastic cells, or both cell populations. The authors conclude that the TCR delta gene is usually deleted in mature T-cell lymphomas, as would be expected in alpha/beta TCR T cells. However, TCR delta gene rearrangement is detectable in approximately 10% of cases. Analysis of this locus may be useful diagnostically, as it occasionally may be the only molecular marker of clonality in mature T-cell lymphomas T-cell receptor delta chain gene rearrangement also is found most often in lymphomas of the large cell anaplastic type.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asou N., Matsuoka M., Hattori T., Kawano F., Maeda S., Shimada K., Takatsuki K. T cell gamma gene rearrangements in hematologic neoplasms. Blood. 1987 Mar;69(3):968–970. [PubMed] [Google Scholar]
  2. Bertness V., Kirsch I., Hollis G., Johnson B., Bunn P. A., Jr T-cell receptor gene rearrangements as clinical markers of human T-cell lymphomas. N Engl J Med. 1985 Aug 29;313(9):534–538. doi: 10.1056/NEJM198508293130902. [DOI] [PubMed] [Google Scholar]
  3. Cossman J., Uppenkamp M. T-cell gene rearrangements and the diagnosis of T-cell neoplasms. Clin Lab Med. 1988 Mar;8(1):31–44. [PubMed] [Google Scholar]
  4. Dyer M. J. T-cell receptor delta/alpha rearrangements in lymphoid neoplasms. Blood. 1989 Aug 15;74(3):1073–1083. [PubMed] [Google Scholar]
  5. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  6. Flug F., Pelicci P. G., Bonetti F., Knowles D. M., 2nd, Dalla-Favera R. T-cell receptor gene rearrangements as markers of lineage and clonality in T-cell neoplasms. Proc Natl Acad Sci U S A. 1985 May;82(10):3460–3464. doi: 10.1073/pnas.82.10.3460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Foroni L., Laffan M., Boehm T., Rabbitts T. H., Catovsky D., Luzzatto L. Rearrangement of the T-cell receptor delta genes in human T-cell leukemias. Blood. 1989 Feb;73(2):559–565. [PubMed] [Google Scholar]
  8. Griesinger F., Greenberg J. M., Kersey J. H. T cell receptor gamma and delta rearrangements in hematologic malignancies. Relationship to lymphoid differentiation. J Clin Invest. 1989 Aug;84(2):506–516. doi: 10.1172/JCI114193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Griesser H., Tkachuk D., Reis M. D., Mak T. W. Gene rearrangements and translocations in lymphoproliferative diseases. Blood. 1989 May 1;73(6):1402–1415. [PubMed] [Google Scholar]
  10. Hara J., Benedict S. H., Champagne E., Takihara Y., Mak T. W., Minden M., Gelfand E. W. T cell receptor delta gene rearrangements in acute lymphoblastic leukemia. J Clin Invest. 1988 Dec;82(6):1974–1982. doi: 10.1172/JCI113817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Herbst H., Tippelmann G., Anagnostopoulos I., Gerdes J., Schwarting R., Boehm T., Pileri S., Jones D. B., Stein H. Immunoglobulin and T-cell receptor gene rearrangements in Hodgkin's disease and Ki-1-positive anaplastic large cell lymphoma: dissociation between phenotype and genotype. Leuk Res. 1989;13(2):103–116. doi: 10.1016/0145-2126(89)90134-3. [DOI] [PubMed] [Google Scholar]
  12. Jaffe E. S., Blattner W. A., Blayney D. W., Bunn P. A., Jr, Cossman J., Robert-Guroff M., Gallo R. C. The pathologic spectrum of adult T-cell leukemia/lymphoma in the United States. Human T-cell leukemia/lymphoma virus-associated lymphoid malignancies. Am J Surg Pathol. 1984 Apr;8(4):263–275. doi: 10.1097/00000478-198404000-00003. [DOI] [PubMed] [Google Scholar]
  13. Kimura N., Takihara Y., Akiyoshi T., Yoshida T., Ohshima K., Kawara T., Hisano S., Kozuru M., Okumura M., Kikuchi M. Rearrangement of T-cell receptor delta chain gene as a marker of lineage and clonality in T-cell lymphoproliferative disorders. Cancer Res. 1989 Aug 15;49(16):4488–4492. [PubMed] [Google Scholar]
  14. Knowles D. M. Immunophenotypic and antigen receptor gene rearrangement analysis in T cell neoplasia. Am J Pathol. 1989 Apr;134(4):761–785. [PMC free article] [PubMed] [Google Scholar]
  15. Lafaille J. J., DeCloux A., Bonneville M., Takagaki Y., Tonegawa S. Junctional sequences of T cell receptor gamma delta genes: implications for gamma delta T cell lineages and for a novel intermediate of V-(D)-J joining. Cell. 1989 Dec 1;59(5):859–870. doi: 10.1016/0092-8674(89)90609-0. [DOI] [PubMed] [Google Scholar]
  16. Lipford E. H., Smith H. R., Pittaluga S., Jaffe E. S., Steinberg A. D., Cossman J. Clonality of angioimmunoblastic lymphadenopathy and implications for its evolution to malignant lymphoma. J Clin Invest. 1987 Feb;79(2):637–642. doi: 10.1172/JCI112860. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Medeiros L. J., Herrington R. D., Gonzalez C. L., Jaffe E. S., Cossman J. My4 antibody staining of non-Hodgkin's lymphomas. Am J Clin Pathol. 1991 Mar;95(3):363–368. doi: 10.1093/ajcp/95.3.363. [DOI] [PubMed] [Google Scholar]
  18. O'Connor N. T., Wainscoat J. S., Weatherall D. J., Gatter K. C., Feller A. C., Isaacson P., Jones D., Lennert K., Pallesen G., Ramsey A. Rearrangement of the T-cell-receptor beta-chain gene in the diagnosis of lymphoproliferative disorders. Lancet. 1985 Jun 8;1(8441):1295–1297. doi: 10.1016/s0140-6736(85)92791-6. [DOI] [PubMed] [Google Scholar]
  19. Patsouris E., Noël H., Lennert K. Histological and immunohistological findings in lymphoepithelioid cell lymphoma (Lennert's lymphoma). Am J Surg Pathol. 1988 May;12(5):341–350. doi: 10.1097/00000478-198805000-00002. [DOI] [PubMed] [Google Scholar]
  20. Picker L. J., Weiss L. M., Medeiros L. J., Wood G. S., Warnke R. A. Immunophenotypic criteria for the diagnosis of non-Hodgkin's lymphoma. Am J Pathol. 1987 Jul;128(1):181–201. [PMC free article] [PubMed] [Google Scholar]
  21. Pittaluga S., Raffeld M., Lipford E. H., Cossman J. 3A1 (CD7) expression precedes T beta gene rearrangements in precursor T (lymphoblastic) neoplasms. Blood. 1986 Jul;68(1):134–139. [PubMed] [Google Scholar]
  22. Reed K. C., Mann D. A. Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res. 1985 Oct 25;13(20):7207–7221. doi: 10.1093/nar/13.20.7207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Smith J. L., Haegert D. G., Hodges E., Stacey G. N., Howell W. M., Wright D. H., Jones D. B. Phenotypic and genotypic heterogeneity of peripheral T-cell lymphoma. Br J Cancer. 1988 Dec;58(6):723–729. doi: 10.1038/bjc.1988.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Stein H., Mason D. Y., Gerdes J., O'Connor N., Wainscoat J., Pallesen G., Gatter K., Falini B., Delsol G., Lemke H. The expression of the Hodgkin's disease associated antigen Ki-1 in reactive and neoplastic lymphoid tissue: evidence that Reed-Sternberg cells and histiocytic malignancies are derived from activated lymphoid cells. Blood. 1985 Oct;66(4):848–858. [PubMed] [Google Scholar]
  25. Takihara Y., Reimann J., Michalopoulos E., Ciccone E., Moretta L., Mak T. W. Diversity and structure of human T cell receptor delta chain genes in peripheral blood gamma/delta-bearing T lymphocytes. J Exp Med. 1989 Feb 1;169(2):393–405. doi: 10.1084/jem.169.2.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tkachuk D. C., Griesser H., Takihara Y., Champagne E., Minden M., Feller A. C., Lennert K., Mak T. W. Rearrangement of T-cell delta locus in lymphoproliferative disorders. Blood. 1988 Jul;72(1):353–357. [PubMed] [Google Scholar]
  27. Uppenkamp M., Andrade R., Sundeen J., Raffeld M., Coupland R., Cossman J. Diagnostic interpretation of T gamma gene rearrangement: effect of polyclonal T cells. Hematol Pathol. 1988;2(1):15–24. [PubMed] [Google Scholar]
  28. Waldmann T. A., Davis M. M., Bongiovanni K. F., Korsmeyer S. J. Rearrangements of genes for the antigen receptor on T cells as markers of lineage and clonality in human lymphoid neoplasms. N Engl J Med. 1985 Sep 26;313(13):776–783. doi: 10.1056/NEJM198509263131303. [DOI] [PubMed] [Google Scholar]
  29. de Villartay J. P., Hockett R. D., Coran D., Korsmeyer S. J., Cohen D. I. Deletion of the human T-cell receptor delta-gene by a site-specific recombination. Nature. 1988 Sep 8;335(6186):170–174. doi: 10.1038/335170a0. [DOI] [PubMed] [Google Scholar]
  30. de Villartay J. P., Pullman A. B., Andrade R., Tschachler E., Colamenici O., Neckers L., Cohen D. I., Cossman J. Gamma/delta lineage relationship within a consecutive series of human precursor T-cell neoplasms. Blood. 1989 Nov 15;74(7):2508–2518. [PubMed] [Google Scholar]

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