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. 1992 Jan;140(1):1–8.

Diversity of T-cell antigen receptor variable genes used by mycosis fungoides cells.

D W Bahler 1, G Berry 1, J Oksenberg 1, R A Warnke 1, R Levy 1
PMCID: PMC1886256  PMID: 1731519

Abstract

The expression of T-cell antigen receptor beta-chain variable genes (V beta) was evaluated in 28 cases of mycosis fungoides. A novel polymerase chain reaction (PCR) technique was used to associate expression of particular V beta genes with monoclonal T-cell populations. In addition, the same biopsies used for PCR analysis were also examined for reactivity with a panel of seven monoclonal antibodies that specifically recognized V beta proteins from four different families. Only three cases clearly stained with the antibodies, a result consistent with a diverse set of V beta genes being used. This was confirmed by PCR analysis, which indicated that V beta genes from many different families were expressed by these tumors. Preferential use of the V beta 8 family, which had been previously use of the V beta 8 family, which had been previously reported for this disease, was not evident among the cases analyzed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bindl J. M., Warnke R. A. Advantages of detecting monoclonal antibody binding to tissue sections with biotin and avidin reagents in Coplin jars. Am J Clin Pathol. 1986 Apr;85(4):490–493. doi: 10.1093/ajcp/85.4.490. [DOI] [PubMed] [Google Scholar]
  2. Boylston A. W., Borst J., Yssel H., Blanchard D., Spits H., de Vries J. E. Properties of a panel of monoclonal antibodies which react with the human T cell antigen receptor on the leukemic line HPB-ALL and a subset of normal peripheral blood T lymphocytes. J Immunol. 1986 Jul 15;137(2):741–744. [PubMed] [Google Scholar]
  3. Bragado R., Lauzurica P., López D., López de Castro J. A. T cell receptor V beta gene usage in a human alloreactive response. Shared structural features among HLA-B27-specific T cell clones. J Exp Med. 1990 Apr 1;171(4):1189–1204. doi: 10.1084/jem.171.4.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark D. M., Boylston A. W., Hall P. A., Carrel S. Antibodies to T cell antigen receptor beta chain families detect monoclonal T cell proliferation. Lancet. 1986 Oct 11;2(8511):835–837. doi: 10.1016/s0140-6736(86)92870-9. [DOI] [PubMed] [Google Scholar]
  5. Dosaka N., Tanaka T., Fujita M., Miyachi Y., Horio T., Imamura S. Southern blot analysis of clonal rearrangements of T-cell receptor gene in plaque lesion of mycosis fungoides. J Invest Dermatol. 1989 Nov;93(5):626–629. doi: 10.1111/1523-1747.ep12319746. [DOI] [PubMed] [Google Scholar]
  6. Ferradini L., Roman-Roman S., Azocar J., Michalaki H., Triebel F., Hercend T. Studies on the human T cell receptor alpha/beta variable region genes. II. Identification of four additional V beta subfamilies. Eur J Immunol. 1991 Apr;21(4):935–942. doi: 10.1002/eji.1830210412. [DOI] [PubMed] [Google Scholar]
  7. Güssow D., Rein R., Ginjaar I., Hochstenbach F., Seemann G., Kottman A., Ploegh H. L. The human beta 2-microglobulin gene. Primary structure and definition of the transcriptional unit. J Immunol. 1987 Nov 1;139(9):3132–3138. [PubMed] [Google Scholar]
  8. Jack A. S., Boylston A. W., Carrel S., Grigor I. Cutaneous T-cell lymphoma cells employ a restricted range of T-cell antigen receptor variable region genes. Am J Pathol. 1990 Jan;136(1):17–21. [PMC free article] [PubMed] [Google Scholar]
  9. Kappler J., Kotzin B., Herron L., Gelfand E. W., Bigler R. D., Boylston A., Carrel S., Posnett D. N., Choi Y., Marrack P. V beta-specific stimulation of human T cells by staphylococcal toxins. Science. 1989 May 19;244(4906):811–813. doi: 10.1126/science.2524876. [DOI] [PubMed] [Google Scholar]
  10. Maecker H. T., Levy R. Prevalence of antigen receptor variants in human T cell lines and tumors. J Immunol. 1989 Feb 15;142(4):1395–1404. [PubMed] [Google Scholar]
  11. Marrack P., Kappler J. The T cell receptor. Science. 1987 Nov 20;238(4830):1073–1079. doi: 10.1126/science.3317824. [DOI] [PubMed] [Google Scholar]
  12. McCarthy K. P., Sloane J. P., Kabarowski J. H., Matutes E., Wiedemann L. M. The rapid detection of clonal T-cell proliferations in patients with lymphoid disorders. Am J Pathol. 1991 Apr;138(4):821–828. [PMC free article] [PubMed] [Google Scholar]
  13. Poppema S., Hepperle B. Restricted V gene usage in T-cell lymphomas as detected by anti-T-cell receptor variable region reagents. Am J Pathol. 1991 Jun;138(6):1479–1484. [PMC free article] [PubMed] [Google Scholar]
  14. Ralfkiaer E., O'Connor N. T., Crick J., Wantzin G. L., Mason D. Y. Genotypic analysis of cutaneous T-cell lymphomas. J Invest Dermatol. 1987 Jun;88(6):762–765. doi: 10.1111/1523-1747.ep12470458. [DOI] [PubMed] [Google Scholar]
  15. Robinson M. A. The human T cell receptor beta-chain gene complex contains at least 57 variable gene segments. Identification of six V beta genes in four new gene families. J Immunol. 1991 Jun 15;146(12):4392–4397. [PubMed] [Google Scholar]
  16. Toyonaga B., Mak T. W. Genes of the T-cell antigen receptor in normal and malignant T cells. Annu Rev Immunol. 1987;5:585–620. doi: 10.1146/annurev.iy.05.040187.003101. [DOI] [PubMed] [Google Scholar]
  17. Toyonaga B., Yoshikai Y., Vadasz V., Chin B., Mak T. W. Organization and sequences of the diversity, joining, and constant region genes of the human T-cell receptor beta chain. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8624–8628. doi: 10.1073/pnas.82.24.8624. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wood G. S., Bourguin A., Crooks C. F., Sklar J. Quantitation of T-cell DNA in cutaneous lymphoid infiltrates. Am J Pathol. 1991 Jun;138(6):1503–1509. [PMC free article] [PubMed] [Google Scholar]
  19. Wood G. S., Dubiel C., Mueller C., Abel E. A., Hoppe R. T., Edinger A., Weissman I., Warnke R. A. Most CD8+ cells in skin lesions of CD3+ CD4+ mycosis fungoides are CD3+ T cells that lack CD11b, CD16, CD56, CD57, and human Hanukah factor mRNA. Am J Pathol. 1991 Jun;138(6):1545–1552. [PMC free article] [PubMed] [Google Scholar]
  20. Wucherpfennig K. W., Ota K., Endo N., Seidman J. G., Rosenzweig A., Weiner H. L., Hafler D. A. Shared human T cell receptor V beta usage to immunodominant regions of myelin basic protein. Science. 1990 May 25;248(4958):1016–1019. doi: 10.1126/science.1693015. [DOI] [PubMed] [Google Scholar]

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