Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1995 Feb;69(2):1271–1274. doi: 10.1128/jvi.69.2.1271-1274.1995

Neutralization of respiratory syncytial virus after cell attachment.

C Osiowy 1, R Anderson 1
PMCID: PMC188702  PMID: 7815504

Abstract

Little is known about the mechanisms of antibody-mediated neutralization of respiratory syncytial virus (RSV) which causes recurrent infections in human despite the virtually universal presence of neutralizing serum antibodies. Human serum neutralization titers showed strong correlation with post-cell-attachment neutralizing titers for both RSV-convalescent sera and control sera but showed less strong correlation with cell-attachment blocking titers. Neutralization was effective for the first 60 min of infection, indicating that immune serum-mediated neutralization of RSV infection largely involves inhibition of early events following cell attachment.

Full Text

The Full Text of this article is available as a PDF (233.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong S. J., Dimmock N. J. Neutralization of influenza virus by low concentrations of hemagglutinin-specific polymeric immunoglobulin A inhibits viral fusion activity, but activation of the ribonucleoprotein is also inhibited. J Virol. 1992 Jun;66(6):3823–3832. doi: 10.1128/jvi.66.6.3823-3832.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bernstein J. M., Hruska J. F. Respiratory syncytial virus proteins: identification by immunoprecipitation. J Virol. 1981 Apr;38(1):278–285. doi: 10.1128/jvi.38.1.278-285.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bruhn F. W., Yeager A. S. Respiratory syncytial virus in early infancy. Circulating antibody and the severity of infection. Am J Dis Child. 1977 Feb;131(2):145–148. doi: 10.1001/archpedi.1977.02120150027004. [DOI] [PubMed] [Google Scholar]
  4. Bächi T. Direct observation of the budding and fusion of an enveloped virus by video microscopy of viable cells. J Cell Biol. 1988 Nov;107(5):1689–1695. doi: 10.1083/jcb.107.5.1689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dietzschold B., Kao M., Zheng Y. M., Chen Z. Y., Maul G., Fu Z. F., Rupprecht C. E., Koprowski H. Delineation of putative mechanisms involved in antibody-mediated clearance of rabies virus from the central nervous system. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7252–7256. doi: 10.1073/pnas.89.15.7252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dimmock N. J. Neutralization of animal viruses. Curr Top Microbiol Immunol. 1993;183:1–149. doi: 10.1007/978-3-642-77849-0. [DOI] [PubMed] [Google Scholar]
  7. Glezen W. P., Paredes A., Allison J. E., Taber L. H., Frank A. L. Risk of respiratory syncytial virus infection for infants from low-income families in relationship to age, sex, ethnic group, and maternal antibody level. J Pediatr. 1981 May;98(5):708–715. doi: 10.1016/s0022-3476(81)80829-3. [DOI] [PubMed] [Google Scholar]
  8. Groothuis J. R., Simoes E. A., Levin M. J., Hall C. B., Long C. E., Rodriguez W. J., Arrobio J., Meissner H. C., Fulton D. R., Welliver R. C. Prophylactic administration of respiratory syncytial virus immune globulin to high-risk infants and young children. The Respiratory Syncytial Virus Immune Globulin Study Group. N Engl J Med. 1993 Nov 18;329(21):1524–1530. doi: 10.1056/NEJM199311183292102. [DOI] [PubMed] [Google Scholar]
  9. Hall C. B., Walsh E. E., Long C. E., Schnabel K. C. Immunity to and frequency of reinfection with respiratory syncytial virus. J Infect Dis. 1991 Apr;163(4):693–698. doi: 10.1093/infdis/163.4.693. [DOI] [PubMed] [Google Scholar]
  10. Kim H. W., Canchola J. G., Brandt C. D., Pyles G., Chanock R. M., Jensen K., Parrott R. H. Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine. Am J Epidemiol. 1969 Apr;89(4):422–434. doi: 10.1093/oxfordjournals.aje.a120955. [DOI] [PubMed] [Google Scholar]
  11. Lamprecht C. L., Krause H. E., Mufson M. A. Role of maternal antibody in pneumonia and bronchiolitis due to respiratory syncytial virus. J Infect Dis. 1976 Sep;134(3):211–217. doi: 10.1093/infdis/134.3.211. [DOI] [PubMed] [Google Scholar]
  12. Levine S., Hamilton R. Kinetics of the respiratory syncytial virus growth cycle in HeLa cells. Arch Gesamte Virusforsch. 1969;28(2):122–132. doi: 10.1007/BF01249378. [DOI] [PubMed] [Google Scholar]
  13. Outlaw M. C., Dimmock N. J. IgG neutralization of type A influenza viruses and the inhibition of the endosomal fusion stage of the infectious pathway in BHK cells. Virology. 1993 Aug;195(2):413–421. doi: 10.1006/viro.1993.1391. [DOI] [PubMed] [Google Scholar]
  14. Outlaw M. C., Dimmock N. J. Insights into neutralization of animal viruses gained from study of influenza virus. Epidemiol Infect. 1991 Apr;106(2):205–220. doi: 10.1017/s0950268800048354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Parrott R. H., Kim H. W., Arrobio J. O., Hodes D. S., Murphy B. R., Brandt C. D., Camargo E., Chanock R. M. Epidemiology of respiratory syncytial virus infection in Washington, D.C. II. Infection and disease with respect to age, immunologic status, race and sex. Am J Epidemiol. 1973 Oct;98(4):289–300. doi: 10.1093/oxfordjournals.aje.a121558. [DOI] [PubMed] [Google Scholar]
  16. Rieber E. P., Federle C., Reiter C., Krauss S., Gürtler L., Eberle J., Deinhardt F., Riethmüller G. The monoclonal CD4 antibody M-T413 inhibits cellular infection with human immunodeficiency virus after viral attachment to the cell membrane: an approach to postexposure prophylaxis. Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10792–10796. doi: 10.1073/pnas.89.22.10792. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rigg R. J., Carver A. S., Dimmock N. J. IgG-neutralized influenza virus undergoes primary, but not secondary uncoating in vivo. J Gen Virol. 1989 Aug;70(Pt 8):2097–2109. doi: 10.1099/0022-1317-70-8-2097. [DOI] [PubMed] [Google Scholar]
  18. Skinner M. A., Langlois A. J., McDanal C. B., McDougal J. S., Bolognesi D. P., Matthews T. J. Neutralizing antibodies to an immunodominant envelope sequence do not prevent gp120 binding to CD4. J Virol. 1988 Nov;62(11):4195–4200. doi: 10.1128/jvi.62.11.4195-4200.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stott E. J., Taylor G. Respiratory syncytial virus. Brief review. Arch Virol. 1985;84(1-2):1–52. doi: 10.1007/BF01310552. [DOI] [PubMed] [Google Scholar]
  20. Tolskaya E. A., Ivannikova T. A., Kolesnikova M. S., Drozdov S. G., Agol V. I. Postinfection treatment with antiviral serum results in survival of neural cells productively infected with virulent poliovirus. J Virol. 1992 Aug;66(8):5152–5156. doi: 10.1128/jvi.66.8.5152-5156.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vrijsen R., Mosser A., Boeyé A. Postabsorption neutralization of poliovirus. J Virol. 1993 Jun;67(6):3126–3133. doi: 10.1128/jvi.67.6.3126-3133.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ward K. A., Lambden P. R., Ogilvie M. M., Watt P. J. Antibodies to respiratory syncytial virus polypeptides and their significance in human infection. J Gen Virol. 1983 Sep;64(Pt 9):1867–1876. doi: 10.1099/0022-1317-64-9-1867. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES