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. 1993 Aug;143(2):555–564.

The demyelinating potential of antibodies to myelin oligodendrocyte glycoprotein is related to their ability to fix complement.

S J Piddlesden 1, H Lassmann 1, F Zimprich 1, B P Morgan 1, C Linington 1
PMCID: PMC1887024  PMID: 7688186

Abstract

A panel of 13 monoclonal antibodies (mAbs) has been raised to the central nervous system-specific glycoprotein, myelin oligodendrocyte glycoprotein; five of these mAbs recognize a carbohydrate epitope on the molecule. Although all of the mAbs recognized surface epitopes on cultured oligodendrocytes and stained central nervous system tissue sections in a similar manner, marked differences were seen in their ability to induce demyelination in experimental allergic encephalomyelitis in the Lewis rat. This variation in pathogenic potential was not related to the specificity of a given mAb for carbohydrate or peptide epitopes of myelin oligodendrocyte glycoprotein, but correlated with its ability to fix complement.

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Selected References

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  1. Brosnan C. F., Bornstein M. B., Bloom B. R. The effects of macrophage depletion on the clinical and pathologic expression of experimental allergic encephalomyelitis. J Immunol. 1981 Feb;126(2):614–620. [PubMed] [Google Scholar]
  2. Brunner C., Lassmann H., Waehneldt T. V., Matthieu J. M., Linington C. Differential ultrastructural localization of myelin basic protein, myelin/oligodendroglial glycoprotein, and 2',3'-cyclic nucleotide 3'-phosphodiesterase in the CNS of adult rats. J Neurochem. 1989 Jan;52(1):296–304. doi: 10.1111/j.1471-4159.1989.tb10930.x. [DOI] [PubMed] [Google Scholar]
  3. Burton D. R. Immunoglobulin G: functional sites. Mol Immunol. 1985 Mar;22(3):161–206. doi: 10.1016/0161-5890(85)90151-8. [DOI] [PubMed] [Google Scholar]
  4. Driscoll B. F., Kira J., Kies M. W., Alvord E. C., Jr Mechanism of demyelination in the guinea pig. Separate sensitization with encephalitogenic myelin basic protein and nonencephalitogenic brain components. Neurochem Pathol. 1986 Feb;4(1):11–22. doi: 10.1007/BF02834295. [DOI] [PubMed] [Google Scholar]
  5. Huitinga I., van Rooijen N., de Groot C. J., Uitdehaag B. M., Dijkstra C. D. Suppression of experimental allergic encephalomyelitis in Lewis rats after elimination of macrophages. J Exp Med. 1990 Oct 1;172(4):1025–1033. doi: 10.1084/jem.172.4.1025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kabat E. A., Wolf A., Bezer A. E. Rapid Production of Acute Disseminated Encephalomyelitis in Rhesus Monkeys by Injection of Brain Tissue With Adjuvants. Science. 1946 Oct 18;104(2703):362–363. doi: 10.1126/science.104.2703.362. [DOI] [PubMed] [Google Scholar]
  7. Kerlero de Rosbo N., Honegger P., Lassmann H., Matthieu J. M. Demyelination induced in aggregating brain cell cultures by a monoclonal antibody against myelin/oligodendrocyte glycoprotein. J Neurochem. 1990 Aug;55(2):583–587. doi: 10.1111/j.1471-4159.1990.tb04173.x. [DOI] [PubMed] [Google Scholar]
  8. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Lassmann H., Brunner C., Bradl M., Linington C. Experimental allergic encephalomyelitis: the balance between encephalitogenic T lymphocytes and demyelinating antibodies determines size and structure of demyelinated lesions. Acta Neuropathol. 1988;75(6):566–576. doi: 10.1007/BF00686201. [DOI] [PubMed] [Google Scholar]
  11. Lassmann H., Stemberger H., Kitz K., Wisniewski H. M. In vivo demyelinating activity of sera from animals with chronic experimental allergic encephalomyelitis. Antibody nature of the demyelinating factor and the role of complement. J Neurol Sci. 1983 Apr;59(1):123–137. doi: 10.1016/0022-510x(83)90086-2. [DOI] [PubMed] [Google Scholar]
  12. Levine S., Cochrane C. G., Carpenter C. B., Behan P. O. Allergic encephalomyelitis: effect of complement depletion with cobra venom. Proc Soc Exp Biol Med. 1971 Oct;138(1):285–289. doi: 10.3181/00379727-138-35880. [DOI] [PubMed] [Google Scholar]
  13. Linington C., Bradl M., Lassmann H., Brunner C., Vass K. Augmentation of demyelination in rat acute allergic encephalomyelitis by circulating mouse monoclonal antibodies directed against a myelin/oligodendrocyte glycoprotein. Am J Pathol. 1988 Mar;130(3):443–454. [PMC free article] [PubMed] [Google Scholar]
  14. Linington C., Lassmann H., Morgan B. P., Compston D. A. Immunohistochemical localisation of terminal complement component C9 in experimental allergic encephalomyelitis. Acta Neuropathol. 1989;79(1):78–85. doi: 10.1007/BF00308961. [DOI] [PubMed] [Google Scholar]
  15. Linington C., Morgan B. P., Scolding N. J., Wilkins P., Piddlesden S., Compston D. A. The role of complement in the pathogenesis of experimental allergic encephalomyelitis. Brain. 1989 Aug;112(Pt 4):895–911. doi: 10.1093/brain/112.4.895. [DOI] [PubMed] [Google Scholar]
  16. Madrid R. E., Wiśniewski H. M., Iqbal K., Pullarkat R. K., Lassmann H. Relapsing experimental allergic encephalomyelitis induced with isolated myelin and with myelin basic protein plus myelin lipids. J Neurol Sci. 1981 Jun;50(3):399–411. doi: 10.1016/0022-510x(81)90152-0. [DOI] [PubMed] [Google Scholar]
  17. Nakane P. K. Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands of the rat. J Histochem Cytochem. 1968 Sep;16(9):557–560. doi: 10.1177/16.9.557. [DOI] [PubMed] [Google Scholar]
  18. Newcombe J., Woodroofe M. N., Cuzner M. L. Distribution of glial fibrillary acidic protein in gliosed human white matter. J Neurochem. 1986 Dec;47(6):1713–1719. doi: 10.1111/j.1471-4159.1986.tb13079.x. [DOI] [PubMed] [Google Scholar]
  19. Norton W. T., Poduslo S. E. Myelination in rat brain: method of myelin isolation. J Neurochem. 1973 Oct;21(4):749–757. doi: 10.1111/j.1471-4159.1973.tb07519.x. [DOI] [PubMed] [Google Scholar]
  20. Pabst H., Day N. K., Gewurz H., Good R. A. Prevention of experimental allergic encephalomyelitis with cobra venom factor. Proc Soc Exp Biol Med. 1971 Feb;136(2):555–560. doi: 10.3181/00379727-136-35310. [DOI] [PubMed] [Google Scholar]
  21. Piddlesden S., Lassmann H., Laffafian I., Morgan B. P., Linington C. Antibody-mediated demyelination in experimental allergic encephalomyelitis is independent of complement membrane attack complex formation. Clin Exp Immunol. 1991 Feb;83(2):245–250. doi: 10.1111/j.1365-2249.1991.tb05622.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Raff M. C., Miller R. H., Noble M. A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium. Nature. 1983 Jun 2;303(5916):390–396. doi: 10.1038/303390a0. [DOI] [PubMed] [Google Scholar]
  23. STONE S. H., LERNER E. M., 2nd CHRONIC DISSEMINATED ALLERGIC ENCEPHALOMYELITIS IN GUINEA PIGS. Ann N Y Acad Sci. 1965 Mar 31;122:227–241. doi: 10.1111/j.1749-6632.1965.tb20206.x. [DOI] [PubMed] [Google Scholar]
  24. Scolding N. J., Frith S., Linington C., Morgan B. P., Campbell A. K., Compston D. A. Myelin-oligodendrocyte glycoprotein (MOG) is a surface marker of oligodendrocyte maturation. J Neuroimmunol. 1989 May;22(3):169–176. doi: 10.1016/0165-5728(89)90014-3. [DOI] [PubMed] [Google Scholar]

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