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. 1993 Sep;143(3):794–803.

The Production of lnterleukin-1 Receptor Antagonist by Human Bronchogenic Carcinoma

Daniel R Smith, Steven L Kunkel, Theodore J Standiford, Stephen W Chensue, Mark W Rolfe, Mark B Orringer, Richard I Whyte, Marie D Burdick, Jean M Danforth, Andrew R Gilbert, Robert M Strieter
PMCID: PMC1887195  PMID: 8362978

Abstract

Bronchogenic carcinoma displays an aggressive clinical course that may reflect a capacity to evade host defenses. We postulated that tumors may elaborate interleukin-1 receptor antagonist protein (IRAP) to escape host interleukin-1-dependent responses. Homogenates of human bronchogenic lung tumors demonstrated significant increases of IRAP compared with normal lung tissue controls (n = 48). There was no significant difference in interleukin-1 β levels between tumor and normal lung tissue. Immunohistochemical staining localized IRAP to tumor cells. Semiquantitative pathological analysis demonstrated a modest inflammatory cell infiltrate with qualitative differences between tumors of different histology. Western blot analysis of tumor homogenates demonstrated several molecular weight forms of IRAP. Finally, antigenic IRAP was detected in supernatants of the human bronchogenic carcinoma cell line (A549) maintained in vitro. These findings illustrate the capacity of bronchogenic tumors to produce and secrete IRAP that may be important in tumor evasion of host defenses.

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Selected References

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