Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1993 Sep;143(3):714–724.

Chromosome aberrations in mesoblastic nephroma.

D E Schofield 1, E J Yunis 1, J A Fletcher 1
PMCID: PMC1887219  PMID: 8395771

Abstract

Mesoblastic nephroma (MN) is the most common renal tumor diagnosed in infancy. Histologically, MNs are designated as classic, cellular, or mixed type based upon variations in cellularity. Recent karyotypic reports have suggested that extra copies of chromosome 11 are a nonrandom occurrence in MNs. We analyzed nuclear suspensions prepared from a group of 17 formalin-fixed, paraffin-embedded tumors to determine the possible role of chromosome 11 copy number in the genesis of MN. Extra copies of D11Z1 (a probe for the centromeric region of chromosome 11) were detected in seven out of 10 MNs with cellular or mixed histology, whereas each of six classic histology MNs were disomic for D11Z1 (P < 0.05). Additional fluorescence in situ hybridization studies utilizing the probes for the alpha satellite, centromeric regions of chromosomes 7, 8, 9, 12, 17, and 20 were then carried out on all cases with cellular or mixed histology. Five out of 10 cellular or mixed MNs had extra copies of D8Z1, and four out of 10 had extra copies of D17Z1, suggesting that gains of chromosomes 8 and 17 may be additional nonrandom cytogenetic events associated with the evolution of MNs. DNA aneuploidy, as determined by image analysis, was detected in three tumors: all had greater than four chromosomal aberrations documented by fluorescence in situ hybridization.

Full text

PDF
714

Images in this article

715Figure 1
on p.715
717Figure 2
on p.717
721Figure 4
on p.721

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adam L. R., Davison E. V., Malcolm A. J., Pearson A. D., Craft A. W. Cytogenetic analysis of a congenital fibrosarcoma. Cancer Genet Cytogenet. 1991 Mar;52(1):37–41. doi: 10.1016/0165-4608(91)90051-u. [DOI] [PubMed] [Google Scholar]
  2. Barrantes J. C., Toyn C., Muir K. R., Parkes S. E., Raafat F., Cameron A. H., Marsden H. B., Mann J. R. Congenital mesoblastic nephroma: possible prognostic and management value of assessing DNA content. J Clin Pathol. 1991 Apr;44(4):317–320. doi: 10.1136/jcp.44.4.317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beckwith J. B., Weeks D. A. Congenital mesoblastic nephroma. When should we worry? Arch Pathol Lab Med. 1986 Feb;110(2):98–99. [PubMed] [Google Scholar]
  4. Bolande R. P., Brough A. J., Izant R. J., Jr Congenital mesoblastic nephroma of infancy. A report of eight cases and the relationship to Wilms' tumor. Pediatrics. 1967 Aug;40(2):272–278. [PubMed] [Google Scholar]
  5. Bolande R. P. Congenital mesoblastic nephroma of infancy. Perspect Pediatr Pathol. 1973;1:227–250. [PubMed] [Google Scholar]
  6. Fletcher J. A., Pinkus G. S., Weidner N., Morton C. C. Lineage-restricted clonality in biphasic solid tumors. Am J Pathol. 1991 May;138(5):1199–1207. [PMC free article] [PubMed] [Google Scholar]
  7. Fu Y., Kay S. Congenital mesoblastic nephroma and its recurrence. Arch Pathol. 1973 Jul;96(1):66–70. [PubMed] [Google Scholar]
  8. Ganick D. J., Gilbert E. F., Beckwith J. B., Kiviat N. Congenital cystic mesoblastic nephroma. Hum Pathol. 1981 Nov;12(11):1039–1043. doi: 10.1016/s0046-8177(81)80265-1. [DOI] [PubMed] [Google Scholar]
  9. Gonzalez-Crussi F., Sotelo-Avila C., Kidd J. M. Malignant mesenchymal nephroma of infancy: report of a case with pulmonary metastases. Am J Surg Pathol. 1980 Apr;4(2):185–190. doi: 10.1097/00000478-198004000-00010. [DOI] [PubMed] [Google Scholar]
  10. Gonzalez-Crussi F., Sotelo-Avila C., Kidd J. M. Mesenchymal renal tumors in infancy: a reappraisal. Hum Pathol. 1981 Jan;12(1):78–85. doi: 10.1016/s0046-8177(81)80244-4. [DOI] [PubMed] [Google Scholar]
  11. Gormley T. S., Skoog S. J., Jones R. V., Maybee D. Cellular congenital mesoblastic nephroma: what are the options. J Urol. 1989 Aug;142(2 Pt 2):479–489. doi: 10.1016/s0022-5347(17)38789-x. [DOI] [PubMed] [Google Scholar]
  12. Haas J. E., Bonadio J. F., Beckwith J. B. Clear cell sarcoma of the kidney with emphasis on ultrastructural studies. Cancer. 1984 Dec 15;54(12):2978–2987. doi: 10.1002/1097-0142(19841215)54:12<2978::aid-cncr2820541228>3.0.co;2-y. [DOI] [PubMed] [Google Scholar]
  13. Hedley D. W., Friedlander M. L., Taylor I. W., Rugg C. A., Musgrove E. A. Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem. 1983 Nov;31(11):1333–1335. doi: 10.1177/31.11.6619538. [DOI] [PubMed] [Google Scholar]
  14. Howell C. G., Othersen H. B., Kiviat N. E., Norkool P., Beckwith J. B., D'Angio G. J. Therapy and outcome in 51 children with mesoblastic nephroma: a report of the National Wilms' Tumor Study. J Pediatr Surg. 1982 Dec;17(6):826–831. doi: 10.1016/s0022-3468(82)80451-x. [DOI] [PubMed] [Google Scholar]
  15. Joshi V. V., Kasznica J., Walters T. R. Atypical mesoblastic nephroma. Pathologic characterization of a potentially aggressive variant of conventional congenital mesoblastic nephroma. Arch Pathol Lab Med. 1986 Feb;110(2):100–106. [PubMed] [Google Scholar]
  16. Joshi V. V., Kay S., Milsten R., Koontz W. W., McWilliams N. B. Congenital mesoblastic nephroma of infancy: report of a case with unusual clinical behavior. Am J Clin Pathol. 1973 Dec;60(6):811–816. doi: 10.1093/ajcp/60.6.811. [DOI] [PubMed] [Google Scholar]
  17. Kaneko Y., Homma C., Maseki N., Sakurai M., Hata J. Correlation of chromosome abnormalities with histological and clinical features in Wilms' and other childhood renal tumors. Cancer Res. 1991 Nov 1;51(21):5937–5942. [PubMed] [Google Scholar]
  18. Kelly D. R. Case 2. Cystic cellular mesoblastic nephroma. Pediatr Pathol. 1985;4(1-2):157–162. doi: 10.3109/15513818509025914. [DOI] [PubMed] [Google Scholar]
  19. Kovacs G., Szücs S., Maschek H. Two chromosomally different cell populations in a partly cellular congenital mesoblastic nephroma. Arch Pathol Lab Med. 1987 Apr;111(4):383–385. [PubMed] [Google Scholar]
  20. Kumar S., Marsden H. B., Carr T., Kodet R. Mesoblastic nephroma contains fibronectin but lacks laminin. J Clin Pathol. 1985 May;38(5):507–511. doi: 10.1136/jcp.38.5.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Limon J., Dal Cin P., Sandberg A. A. Application of long-term collagenase disaggregation for the cytogenetic analysis of human solid tumors. Cancer Genet Cytogenet. 1986 Dec;23(4):305–313. doi: 10.1016/0165-4608(86)90013-0. [DOI] [PubMed] [Google Scholar]
  22. Mandahl N., Heim S., Rydholm A., Willen H., Mitelman F. Nonrandom numerical chromosome aberrations (+8, +11, +17, +20) in infantile fibrosarcoma. Cancer Genet Cytogenet. 1989 Jul 1;40(1):137–139. doi: 10.1016/0165-4608(89)90156-8. [DOI] [PubMed] [Google Scholar]
  23. Mierau G. W., Beckwith J. B., Weeks D. A. Ultrastructure and histogenesis of the renal tumors of childhood: an overview. Ultrastruct Pathol. 1987;11(2-3):313–333. doi: 10.3109/01913128709048329. [DOI] [PubMed] [Google Scholar]
  24. Pettinato G., Manivel J. C., Wick M. R., Dehner L. P. Classical and cellular (atypical) congenital mesoblastic nephroma: a clinicopathologic, ultrastructural, immunohistochemical, and flow cytometric study. Hum Pathol. 1989 Jul;20(7):682–690. doi: 10.1016/0046-8177(89)90156-1. [DOI] [PubMed] [Google Scholar]
  25. Punnett H. H., Halligan G. E., Zaeri N., Karmazin N. Translocation 10;17 in clear cell sarcoma of the kidney. A first report. Cancer Genet Cytogenet. 1989 Aug;41(1):123–128. doi: 10.1016/0165-4608(89)90116-7. [DOI] [PubMed] [Google Scholar]
  26. Richmond H., Dougall A. J. Neonatal renal tumors. J Pediatr Surg. 1970 Aug;5(4):413–418. doi: 10.1016/0022-3468(70)90071-0. [DOI] [PubMed] [Google Scholar]
  27. Sandstedt B., Delemarre J. F., Krul E. J., Tournade M. F. Mesoblastic nephromas: a study of 29 tumours from the SIOP nephroblastoma file. Histopathology. 1985 Jul;9(7):741–750. doi: 10.1111/j.1365-2559.1985.tb02860.x. [DOI] [PubMed] [Google Scholar]
  28. Schmidt D., Wiedemann B., Keil W., Sprenger E., Harms D. Flow cytometric analysis of nephroblastomas and related neoplasms. Cancer. 1986 Dec 1;58(11):2494–2500. doi: 10.1002/1097-0142(19861201)58:11<2494::aid-cncr2820581124>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  29. Schofield D. E., Fletcher J. A. Trisomy 12 in pediatric granulosa-stromal cell tumors. Demonstration by a modified method of fluorescence in situ hybridization on paraffin-embedded material. Am J Pathol. 1992 Dec;141(6):1265–1269. [PMC free article] [PubMed] [Google Scholar]
  30. Seabright M. A rapid banding technique for human chromosomes. Lancet. 1971 Oct 30;2(7731):971–972. doi: 10.1016/s0140-6736(71)90287-x. [DOI] [PubMed] [Google Scholar]
  31. Shen S. C., Yunis E. J. A study of the cellularity and ultrastructure of congenital mesoblastic nephroma. Cancer. 1980 Jan 15;45(2):306–314. doi: 10.1002/1097-0142(19800115)45:2<306::aid-cncr2820450218>3.0.co;2-x. [DOI] [PubMed] [Google Scholar]
  32. Sheng W. W., Soukup S., Bove K., Gotwals B., Lampkin B. Chromosome analysis of 31 Wilms' tumors. Cancer Res. 1990 May 1;50(9):2786–2793. [PubMed] [Google Scholar]
  33. Snyder H. M., 3rd, Lack E. E., Chetty-Baktavizian A., Bauer S. B., Colodny A. H., Retik A. B. Congenital mesoblastic nephroma: relationship to other renal tumors of infancy. J Urol. 1981 Oct;126(4):513–516. doi: 10.1016/s0022-5347(17)54601-7. [DOI] [PubMed] [Google Scholar]
  34. Solis V., Pritchard J., Cowell J. K. Cytogenetic changes in Wilms' tumors. Cancer Genet Cytogenet. 1988 Sep;34(2):223–234. doi: 10.1016/0165-4608(88)90264-6. [DOI] [PubMed] [Google Scholar]
  35. Speleman F., Dal Cin P., De Potter K., Laureys G., Roels H. J., Leroy J., Van Den Berghe H. Cytogenetic investigation of a case of congenital fibrosarcoma. Cancer Genet Cytogenet. 1989 May;39(1):21–24. doi: 10.1016/0165-4608(89)90224-0. [DOI] [PubMed] [Google Scholar]
  36. Steinfeld A. D., Crowley C. A., O'Shea P. A., Tefft M. Recurrent and metastatic mesoblastic nephroma in infancy. J Clin Oncol. 1984 Aug;2(8):956–960. doi: 10.1200/JCO.1984.2.8.956. [DOI] [PubMed] [Google Scholar]
  37. Teyssier J. R., Ferre D. Frequent clonal chromosomal changes in human non-malignant tumors. Int J Cancer. 1989 Nov 15;44(5):828–832. doi: 10.1002/ijc.2910440514. [DOI] [PubMed] [Google Scholar]
  38. Tomlinson G. E., Argyle J. C., Velasco S., Nisen P. D. Molecular characterization of congenital mesoblastic nephroma and its distinction from Wilms tumor. Cancer. 1992 Nov 1;70(9):2358–2361. doi: 10.1002/1097-0142(19921101)70:9<2358::aid-cncr2820700925>3.0.co;2-f. [DOI] [PubMed] [Google Scholar]
  39. Varsa E. W., McConnell T. S., Dressler L. G., Duncan M. Atypical congenital mesoblastic nephroma. Report of a case with karyotypic and flow cytometric analysis. Arch Pathol Lab Med. 1989 Sep;113(9):1078–1080. [PubMed] [Google Scholar]
  40. Walker D., Richard G. A. Fetal hamartoma of the kidney: recurrence and death of patient. J Urol. 1973 Sep;110(3):352–353. doi: 10.1016/s0022-5347(17)60214-3. [DOI] [PubMed] [Google Scholar]
  41. Wigger H. J. Fetal hamartoma of kidney. A benign, symptomatic, congenital tumor, not a form of Wilms' tumor. Am J Clin Pathol. 1969 Mar;51(3):323–337. doi: 10.1093/ajcp/51.3.323. [DOI] [PubMed] [Google Scholar]
  42. Wigger H. J. Fetal mesenchymal hamartoma of kidney. A tumor of secondary mesenchyme. Cancer. 1975 Sep;36(3):1002–1008. doi: 10.1002/1097-0142(197509)36:3<1002::aid-cncr2820360324>3.0.co;2-3. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES