Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1993 Dec;143(6):1535–1542.

Detection of anti-sense transcripts of the insulin-like growth factor-2 gene in Wilms' tumor.

P Baccarini 1, M Fiorentino 1, A D'Errico 1, A M Mancini 1, W F Grigioni 1
PMCID: PMC1887269  PMID: 8256846

Abstract

The expression of the insulin-like growth factor-2 (IGF-2) gene was studied by means of both in situ hybridization and immunohistochemistry in eight cases of Wilms' tumor with different histological features. An anti-sense cRNA IGF-2 probe revealed the presence of abundant IGF-2 mRNAs in all eight tumors examined, localized in the most undifferentiated tumoral cells (blastemal cells); none of the tumors showed immunoreactivity for the anti-IGF-2 antibody. Using a sense cRNA IGF-2 probe, we also detected anti-sense transcripts of the IGF-2 gene in five of eight tumors. These transcripts were exclusively localized in the cells expressing the IGF-2 mRNAs. Although the function of these anti-sense transcripts is unknown, we think that their presence could explain the lack of IGF-2 peptides in Wilms' tumors despite increased expression of IGF-2 mRNAs.

Full text

PDF
1535

Images in this article

1538Figure 1
on p.1538
1539Figure 2
on p.1539
1540Figure 3
on p.1540

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adelman J. P., Bond C. T., Douglass J., Herbert E. Two mammalian genes transcribed from opposite strands of the same DNA locus. Science. 1987 Mar 20;235(4795):1514–1517. doi: 10.1126/science.3547652. [DOI] [PubMed] [Google Scholar]
  2. Beckwith J. B., Kiviat N. B., Bonadio J. F. Nephrogenic rests, nephroblastomatosis, and the pathogenesis of Wilms' tumor. Pediatr Pathol. 1990;10(1-2):1–36. doi: 10.3109/15513819009067094. [DOI] [PubMed] [Google Scholar]
  3. Beckwith J. B. Wilms' tumor and other renal tumors of childhood: a selective review from the National Wilms' Tumor Study Pathology Center. Hum Pathol. 1983 Jun;14(6):481–492. doi: 10.1016/s0046-8177(83)80003-3. [DOI] [PubMed] [Google Scholar]
  4. Brown A. L., Graham D. E., Nissley S. P., Hill D. J., Strain A. J., Rechler M. M. Developmental regulation of insulin-like growth factor II mRNA in different rat tissues. J Biol Chem. 1986 Oct 5;261(28):13144–13150. [PubMed] [Google Scholar]
  5. Cariani E., Seurin D., Lasserre C., Franco D., Binoux M., Brechot C. Expression of insulin-like growth factor II (IGF-II) in human primary liver cancer: mRNA and protein analysis. J Hepatol. 1990 Sep;11(2):226–231. doi: 10.1016/0168-8278(90)90118-b. [DOI] [PubMed] [Google Scholar]
  6. Dull T. J., Gray A., Hayflick J. S., Ullrich A. Insulin-like growth factor II precursor gene organization in relation to insulin gene family. 1984 Aug 30-Sep 5Nature. 310(5980):777–781. doi: 10.1038/310777a0. [DOI] [PubMed] [Google Scholar]
  7. El-Badry O. M., Helman L. J., Chatten J., Steinberg S. M., Evans A. E., Israel M. A. Insulin-like growth factor II-mediated proliferation of human neuroblastoma. J Clin Invest. 1991 Feb;87(2):648–657. doi: 10.1172/JCI115042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Froesch E. R., Schmid C., Schwander J., Zapf J. Actions of insulin-like growth factors. Annu Rev Physiol. 1985;47:443–467. doi: 10.1146/annurev.ph.47.030185.002303. [DOI] [PubMed] [Google Scholar]
  9. Han V. K., D'Ercole A. J., Lund P. K. Cellular localization of somatomedin (insulin-like growth factor) messenger RNA in the human fetus. Science. 1987 Apr 10;236(4798):193–197. doi: 10.1126/science.3563497. [DOI] [PubMed] [Google Scholar]
  10. Haselbacher G. K., Irminger J. C., Zapf J., Ziegler W. H., Humbel R. E. Insulin-like growth factor II in human adrenal pheochromocytomas and Wilms tumors: expression at the mRNA and protein level. Proc Natl Acad Sci U S A. 1987 Feb;84(4):1104–1106. doi: 10.1073/pnas.84.4.1104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hirvonen H., Sandberg M., Kalimo H., Hukkanen V., Vuorio E., Salmi T. T., Alitalo K. The N-myc proto-oncogene and IGF-II growth factor mRNAs are expressed by distinct cells in human fetal kidney and brain. J Cell Biol. 1989 Mar;108(3):1093–1104. doi: 10.1083/jcb.108.3.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Humbel R. E. Insulin-like growth factors I and II. Eur J Biochem. 1990 Jul 5;190(3):445–462. doi: 10.1111/j.1432-1033.1990.tb15595.x. [DOI] [PubMed] [Google Scholar]
  13. Irminger J. C., Schoenle E. J., Briner J., Humbel R. E. Structural alteration of the insulin-like growth factor II-gene in Wilms tumour. Eur J Pediatr. 1989 Jun;148(7):620–623. doi: 10.1007/BF00441514. [DOI] [PubMed] [Google Scholar]
  14. Kimelman D., Kirschner M. W. An antisense mRNA directs the covalent modification of the transcript encoding fibroblast growth factor in Xenopus oocytes. Cell. 1989 Nov 17;59(4):687–696. doi: 10.1016/0092-8674(89)90015-9. [DOI] [PubMed] [Google Scholar]
  15. Kindy M. S., McCormack J. E., Buckler A. J., Levine R. A., Sonenshein G. E. Independent regulation of transcription of the two strands of the c-myc gene. Mol Cell Biol. 1987 Aug;7(8):2857–2862. doi: 10.1128/mcb.7.8.2857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Koufos A., Hansen M. F., Lampkin B. C., Workman M. L., Copeland N. G., Jenkins N. A., Cavenee W. K. Loss of alleles at loci on human chromosome 11 during genesis of Wilms' tumour. Nature. 1984 May 10;309(5964):170–172. doi: 10.1038/309170a0. [DOI] [PubMed] [Google Scholar]
  17. Lamas E., Le Bail B., Housset C., Boucher O., Bréchot C. Localization of insulin-like growth factor-II and hepatitis B virus mRNAs and proteins in human hepatocellular carcinomas. Lab Invest. 1991 Jan;64(1):98–104. [PubMed] [Google Scholar]
  18. Lambert S., Vivario J., Boniver J., Gol-Winkler R. Abnormal expression and structural modification of the insulin-like growth-factor-II gene in human colorectal tumors. Int J Cancer. 1990 Sep 15;46(3):405–410. doi: 10.1002/ijc.2910460313. [DOI] [PubMed] [Google Scholar]
  19. Lazar M. A., Hodin R. A., Darling D. S., Chin W. W. A novel member of the thyroid/steroid hormone receptor family is encoded by the opposite strand of the rat c-erbA alpha transcriptional unit. Mol Cell Biol. 1989 Mar;9(3):1128–1136. doi: 10.1128/mcb.9.3.1128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Macaulay V. M. Insulin-like growth factors and cancer. Br J Cancer. 1992 Mar;65(3):311–320. doi: 10.1038/bjc.1992.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mizuno T., Chou M. Y., Inouye M. A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA). Proc Natl Acad Sci U S A. 1984 Apr;81(7):1966–1970. doi: 10.1073/pnas.81.7.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Paik S., Rosen N., Jung W., You J. M., Lippman M. E., Perdue J. F., Yee D. Expression of insulin-like growth factor-II mRNA in fetal kidney and Wilms' tumor. An in situ hybridization study. Lab Invest. 1989 Nov;61(5):522–526. [PubMed] [Google Scholar]
  23. Reeve A. E., Eccles M. R., Wilkins R. J., Bell G. I., Millow L. J. Expression of insulin-like growth factor-II transcripts in Wilms' tumour. Nature. 1985 Sep 19;317(6034):258–260. doi: 10.1038/317258a0. [DOI] [PubMed] [Google Scholar]
  24. Scott J., Cowell J., Robertson M. E., Priestley L. M., Wadey R., Hopkins B., Pritchard J., Bell G. I., Rall L. B., Graham C. F. Insulin-like growth factor-II gene expression in Wilms' tumour and embryonic tissues. Nature. 1985 Sep 19;317(6034):260–262. doi: 10.1038/317260a0. [DOI] [PubMed] [Google Scholar]
  25. Suzuki T., Iwafuchi M., Takahashi H., Ikuta F., Nishikawa K., Tanaka H., Yanaihara N. Immunocytochemical demonstration of IGF-II-like immunoreactivity in human paraganglioma of the craniocervical region. Virchows Arch A Pathol Anat Histopathol. 1989;414(6):515–521. doi: 10.1007/BF00781709. [DOI] [PubMed] [Google Scholar]
  26. Taylor E. R., Seleiro E. A., Brickell P. M. Identification of antisense transcripts of the chicken insulin-like growth factor-II gene. J Mol Endocrinol. 1991 Oct;7(2):145–154. doi: 10.1677/jme.0.0070145. [DOI] [PubMed] [Google Scholar]
  27. Zumkeller W., Schofield P. N. The role of insulin-like growth factors and IGF-binding proteins in the physiological and pathological processes of the kidney. Virchows Arch B Cell Pathol Incl Mol Pathol. 1992;62(4):207–220. doi: 10.1007/BF02899684. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES