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. 1985 Jan;118(1):15–25.

Interaction between enteroviruses and human endothelial cells in vitro. Alterations in the physical properties of endothelial cell plasma membrane and adhesion of human granulocytes.

C J Kirkpatrick, B D Bültmann, H Gruler
PMCID: PMC1887841  PMID: 2981472

Abstract

Fluorescent molecular probes were used for study of the interaction between enteroviruses (Echo 9, Echo 12, and Coxsackie B3 virus) and human endothelial cells in monolayer culture. With the use of the monomer-excimer method with pyrene decanoic acid it was shown that a marked dose-dependent restructuring of the plasma membrane occurred following addition of virus to the endothelial cells. This took the form of an increase in the lipid surface available to the lipophil reporter molecules, probably due to an alteration in the domain structure of the plasma membrane caused by insertion of virus capsid proteins. Experiments with diphenylhexatriene indicated that the enteroviruses had only a slight tendency to make the plasma membrane of the endothelial cell more fluid. Concomitant with these alterations in the biophysical properties of the membrane, a virus-induced increase in granulocyte adherence to the endothelial cells was observed for all three enteroviruses studied. Possible mechanisms for this elevated adherence are discussed, as well as the significance of the results for the phenomenon of virus-induced granulocytopenia.

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Selected References

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  1. ATHENS J. W., RAAB S. O., HAAB O. P., MAUER A. M., ASHENBRUCKER H., CARTWRIGHT G. E., WINTROBE M. M. Leukokinetic studies. III. The distribution of granulocytes in the blood of normal subjects. J Clin Invest. 1961 Jan;40:159–164. doi: 10.1172/JCI104230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bültmann B. D., Allmendinger P., Raus R. U., Melzner I., Haferkamp O., Eggers H., Gruler H. F-Met-Leu-Phe and echo 9 virus interaction with human granulocytes. Changes of cell membrane structure. Am J Pathol. 1984 Jul;116(1):46–55. [PMC free article] [PubMed] [Google Scholar]
  3. Bültmann B., Eggers H. J., Haferkamp O. Selective inhibition of human neutrophil chemotaxis by ECHO-virus, type 9. Klin Wochenschr. 1981 Jun 1;59(11):571–573. doi: 10.1007/BF01716459. [DOI] [PubMed] [Google Scholar]
  4. Crowell R. L., Philipson L. Specific alterations of coxsackievirus B3 eluted from HeLa cells. J Virol. 1971 Oct;8(4):509–515. doi: 10.1128/jvi.8.4.509-515.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EGGERS H. J., SABIN A. B. Factors determining pathogenicity of variants of ECHO 9 virus for newborn mice. J Exp Med. 1959 Dec 1;110:951–967. doi: 10.1084/jem.110.6.951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. EGGERS H. J., TAMM I. Spectrum and characteristics of the virus inhibitory action of 2-(alpha-hydroxybenzyl)-benzimidazole. J Exp Med. 1961 Apr 1;113:657–682. doi: 10.1084/jem.113.4.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FENWICK M. L., COOPER P. D. Early interactions between poliovirus and ERK cells: some observations on the nature and significance of the rejected particles. Virology. 1962 Oct;18:212–223. doi: 10.1016/0042-6822(62)90007-7. [DOI] [PubMed] [Google Scholar]
  8. Galla H. J., Hartmann W. Excimer-forming lipids in membrane research. Chem Phys Lipids. 1980 Oct;27(3):199–219. doi: 10.1016/0009-3084(80)90036-5. [DOI] [PubMed] [Google Scholar]
  9. Galla H. J., Sackmann E. Lateral diffusion in the hydrophobic region of membranes: use of pyrene excimers as optical probes. Biochim Biophys Acta. 1974 Feb 26;339(1):103–115. doi: 10.1016/0005-2736(74)90336-8. [DOI] [PubMed] [Google Scholar]
  10. Heyn M. P. Determination of lipid order parameters and rotational correlation times from fluorescence depolarization experiments. FEBS Lett. 1979 Dec 15;108(2):359–364. doi: 10.1016/0014-5793(79)80564-5. [DOI] [PubMed] [Google Scholar]
  11. Jaffe E. A., Nachman R. L., Becker C. G., Minick C. R. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 1973 Nov;52(11):2745–2756. doi: 10.1172/JCI107470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jones G., Hurley J. V. The effect of prostacyclin on the adhesion of leucocytes to injured vascular endothelium. J Pathol. 1984 Jan;142(1):51–59. doi: 10.1002/path.1711420110. [DOI] [PubMed] [Google Scholar]
  13. Jähnig F. Structural order of lipids and proteins in membranes: evaluation of fluorescence anisotropy data. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6361–6365. doi: 10.1073/pnas.76.12.6361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Karnovsky M. J., Kleinfeld A. M., Hoover R. L., Klausner R. D. The concept of lipid domains in membranes. J Cell Biol. 1982 Jul;94(1):1–6. doi: 10.1083/jcb.94.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kohn A. Early interactions of viruses with cellular membranes. Adv Virus Res. 1979;24:223–276. doi: 10.1016/s0065-3527(08)60395-4. [DOI] [PubMed] [Google Scholar]
  16. Levanon A., Kohn A. Changes in cell membrane microviscosity associated with adsorption of viruses. Differences between fusing and non-fusing viruses. FEBS Lett. 1978 Jan 15;85(2):245–248. doi: 10.1016/0014-5793(78)80465-7. [DOI] [PubMed] [Google Scholar]
  17. Levanon A., Kohn A., Inbar M. Increase in lipid fluidity of cellular membranes induced by adsorption of RNA and DNA virions. J Virol. 1977 May;22(2):353–360. doi: 10.1128/jvi.22.2.353-360.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. MIMS C. A. ASPECTS OF THE PATHOGENESIS OF VIRUS DISEASES. Bacteriol Rev. 1964 Mar;28:30–71. doi: 10.1128/br.28.1.30-71.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. MacGregor R. R., Friedman H. M., Macarak E. J., Kefalides N. A. Virus infection of endothelial cells increases granulocyte adherence. J Clin Invest. 1980 Jun;65(6):1469–1477. doi: 10.1172/JCI109811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rosenwirth B., Eggers H. J. Early processes of echovirus 12-infection: elution, penetration, and uncoating under the influence of rhodanine. Virology. 1979 Sep;97(2):241–255. doi: 10.1016/0042-6822(79)90336-2. [DOI] [PubMed] [Google Scholar]
  21. Rosenwirth B., Eggers H. J. Structure and replication of echovirus type 12. 1. Analysis of the polypeptides and RNA of echovirus 12 particles. Eur J Biochem. 1978 Dec 1;92(1):53–60. doi: 10.1111/j.1432-1033.1978.tb12722.x. [DOI] [PubMed] [Google Scholar]
  22. Shinitzky M., Barenholz Y. Fluidity parameters of lipid regions determined by fluorescence polarization. Biochim Biophys Acta. 1978 Dec 15;515(4):367–394. doi: 10.1016/0304-4157(78)90010-2. [DOI] [PubMed] [Google Scholar]
  23. Shinitzky M., Inbar M. Microviscosity parameters and protein mobility in biological membranes. Biochim Biophys Acta. 1976 Apr 16;433(1):133–149. doi: 10.1016/0005-2736(76)90183-8. [DOI] [PubMed] [Google Scholar]
  24. Singer S. J., Nicolson G. L. The fluid mosaic model of the structure of cell membranes. Science. 1972 Feb 18;175(4023):720–731. doi: 10.1126/science.175.4023.720. [DOI] [PubMed] [Google Scholar]

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