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. 1985 Jan;118(1):128–133.

Byssinosis: Release of prostaglandins, thromboxane, and 5-hydroxytryptamine in bronchopulmonary lavage fluid after inhalation of cotton dust extracts.

T G Mundie, C Whitener, S K Ainsworth
PMCID: PMC1887862  PMID: 3966532

Abstract

New Zealand White rabbits were exposed intratracheally to aerosolized cotton dust extract (CDE) for 5 minutes of tidal breathing and lavaged 15 minutes 1, 4, and 6 hours after exposure. Bronchoalveolar lavage cells were counted, and the number of macrophages and polymorphonuclear leukocytes (PMNs) was determined. Cell recruitment, which began 1 hour after exposure to CDE and plateaued at 6 hours, consisted of both mononuclear cells and PMNs. Lavage fluid was analyzed for concentrations of prostaglandin F2 alpha (PGF2 alpha), prostaglandin E1 and E2 (PGE), thromboxane B2 (TxB2), and 5-hydroxytryptamine (5-HT). PGF2 alpha, PGE, TxB2, and 5-HT were maximally increased in the lavage 4 hours after exposure to CDE. This is the first study to demonstrate the in vivo release of arachidonic acid metabolites and 5-HT in the lung in response to CDE inhalation. This study also demonstrates that maximum mediator release occurs at 4 hours after exposure to aerosolized CDE. These findings strongly suggest that arachidonic acid metabolites are available to mediate either totally or partially the pathogenic mechanism(s) of bronchoconstriction seen in the acute byssinotic reaction of man.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ainsworth S. K., Neuman R. E. Chemotaxins in cotton mill dust: possible etiologic agent(s) in byssinosis. Am Rev Respir Dis. 1981 Sep;124(3):280–284. doi: 10.1164/arrd.1981.124.3.280. [DOI] [PubMed] [Google Scholar]
  2. Ainsworth S. K., Neuman R. E., Harley R. A. Histamine release from platelets for assay of byssinogenic substances in cotton mill dust and related materials. Br J Ind Med. 1979 Feb;36(1):35–42. doi: 10.1136/oem.36.1.35. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benveniste J., Henson P. M., Cochrane C. G. Leukocyte-dependent histamine release from rabbit platelets. The role of IgE, basophils, and a platelet-activating factor. J Exp Med. 1972 Dec 1;136(6):1356–1377. doi: 10.1084/jem.136.6.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Benveniste J. Platelet-activating factor, a new mediator of anaphylaxis and immune complex deposition from rabbit and human basophils. Nature. 1974 Jun 7;249(457):581–582. doi: 10.1038/249581a0. [DOI] [PubMed] [Google Scholar]
  5. Bomski H., Otawski J., Bomska H. Hämatologische und serologische Untersuchungen bei Byssinose-gefährdeten Arbeitern. Int Arch Arbeitsmed. 1971;27(4):309–323. [PubMed] [Google Scholar]
  6. Cavagna G., Foà V., Vigliani E. C. Effects in man and rabbits of inhalation of cotton dust or extracts and purified endotoxins. Br J Ind Med. 1969 Oct;26(4):314–321. doi: 10.1136/oem.26.4.314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fowler S. R., Ziprin R. L., Elissalde M. H., Jr, Greenblatt G. A. The etiology of byssinosis--possible role of prostaglandin F2alpha synthesis by alveolar macrophages. Am Ind Hyg Assoc J. 1981 Jun;42(6):445–448. doi: 10.1080/15298668191420035. [DOI] [PubMed] [Google Scholar]
  8. Henson P. M. Release of vasoactive amines from rabbit platelets induced by sensitized mononuclear leukocytes and antigen. J Exp Med. 1970 Feb;131(2):287–306. doi: 10.1084/jem.131.2.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Holt P. G., Holt B. J., Beijer L., Rylander R. Platelet serotonin release by human polymorphonuclear leucocytes stimulated by cotton dust bacteria. Clin Exp Immunol. 1983 Jan;51(1):185–190. [PMC free article] [PubMed] [Google Scholar]
  10. Hopkins N. K., Sun F. F., Gorman R. R. Thromboxane A2 biosynthesis in human lung fibroblasts WI-38. Biochem Biophys Res Commun. 1978 Nov 29;85(2):827–836. doi: 10.1016/0006-291x(78)91237-8. [DOI] [PubMed] [Google Scholar]
  11. Issekutz A. C., Movat H. Z. The effect of vasodilator prostaglandins on polymorphonuclear leukocyte infiltration and vascular injury. Am J Pathol. 1982 Jun;107(3):300–309. [PMC free article] [PubMed] [Google Scholar]
  12. Johnson A. R., Callahan K. S., Tsai S. C., Campbell W. B. Prostacyclin and prostaglandin biosynthesis in human pulmonary endothelial cells. Bull Eur Physiopathol Respir. 1981;17(4):531–551. [PubMed] [Google Scholar]
  13. Kilburn K. H., Lynn W. S., Tres L. L., McKenzie W. N. Leukocyte recruitment through airway walls by condensed vegetable tannins and quercetin. Lab Invest. 1973 Jan;28(1):55–59. [PubMed] [Google Scholar]
  14. Kutz S. A., Olenchock S. A., Elliott J. A., Pearson D. J., Major P. C. Antibody-independent complement activation by cardroom cotton dust. Environ Res. 1979 Aug;19(2):405–414. doi: 10.1016/0013-9351(79)90065-3. [DOI] [PubMed] [Google Scholar]
  15. Merchant J. A., Halprin G. M., Hudson A. R., Kilburn K. H., McKenzie W. N., Hurst D. J., Bermazohn P. Responses to cotton dust. Arch Environ Health. 1975 May;30(5):222–229. doi: 10.1080/00039896.1975.10666685. [DOI] [PubMed] [Google Scholar]
  16. Mundie T. G., Cordova-Salinas M., Bray V. J., Ainsworth S. K. Bioassays of smooth muscle contracting agents in cotton mill dust and bract extracts: arachidonic acid metabolites as possible mediators of the acute byssinotic reaction. Environ Res. 1983 Oct;32(1):62–71. doi: 10.1016/0013-9351(83)90192-5. [DOI] [PubMed] [Google Scholar]
  17. Oehling A., Gonzalez de la Reguera I., Viñes Rueda J. J. A contribution to the allergic ethiopathogenity of byssinosis. Respiration. 1972;29(2):155–160. doi: 10.1159/000192870. [DOI] [PubMed] [Google Scholar]
  18. Rohrbach M. S., Rolstad R. A., Tracy P. B., Russell J. A. Platelet 5-hydroxytryptamine release and aggregation promoted by cotton bracts tannin. J Lab Clin Med. 1984 Jan;103(1):152–160. [PubMed] [Google Scholar]
  19. Rylander R., Nordstrand A. Pulmonary cell reactions after exposure to cotton dust extract. Br J Ind Med. 1974 Jul;31(3):220–223. doi: 10.1136/oem.31.3.220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Smith A. P., Cuthbert M. F. Prostaglandins and resistance to beta adrenoceptor stimulants. Br Med J. 1972 Apr 15;2(5806):166–166. doi: 10.1136/bmj.2.5806.166-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Svenssen J., Strandberg K., Tuvemo T., Hamberg M. Thromboxane A2: effects on airway and vascular smooth muscle. Prostaglandins. 1977 Sep;14(3):425–436. doi: 10.1016/0090-6980(77)90258-1. [DOI] [PubMed] [Google Scholar]
  22. Vargaftig B. B., Lefort J., Chignard M., Benveniste J. Platelet-activating factor induces a platelet-dependent bronchoconstriction unrelated to the formation of prostaglandin derivatives. Eur J Pharmacol. 1980 Jul 25;65(2-3):185–192. doi: 10.1016/0014-2999(80)90391-x. [DOI] [PubMed] [Google Scholar]
  23. Walker R. F., Eidson G., Hatcher J. D. Influence of cotton dust inhalation on free lung cells in rats and guinea pigs. Lab Invest. 1975 Jul;33(1):28–32. [PubMed] [Google Scholar]

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