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. 1995 Mar;69(3):1917–1919. doi: 10.1128/jvi.69.3.1917-1919.1995

Packaging in a yeast double-stranded RNA virus.

W Yao 1, K Muqtadir 1, J A Bruenn 1
PMCID: PMC188807  PMID: 7853534

Abstract

The yeast virus ScV-L1 has only two genes, cap and pol, which encode the capsid polypeptide and the viral polymerase, respectively. The second gene is translated only as a cap-pol fusion protein. This fusion protein is responsible for recognition of a specific small stem and loop region of the viral plus strands, of 19 to 31 bases in length, ensuring packaging specificity. We have used a related virus, ScV-La, which has about 29% codon identity with ScV-L1 in the most conserved region of the pol gene, to map the region in pol that is responsible for packaging L1. Characterization of a number of chimeric viral proteins that recognize L1 but have the La capsid region delimits the region necessary for recognition of L1 to a 76- to 82-codon portion of pol. In addition, we show that overproduction of the La capsid polypeptide results in curing of the ScV-La virus, analogous to the production of plants resistant to RNA viruses by virtue of systemic production of viral coat protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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