Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1986 Feb;122(2):308–314.

Experimental transmission of the delta virus to a hepatitis B chronic carrier chimpanzee with the development of persistent delta carriage.

H A Fields, S Govindarajan, H S Margolis, C D Schable, J W Ebert, J E Maynard
PMCID: PMC1888111  PMID: 3511725

Abstract

A delta virus (DV) infection was experimentally transmitted to a hepatitis B chronic carrier chimpanzee. The infection was monitored by the examination of liver biopsy materials with the use of FITC-labeled human anti-delta IgG or by an indirect immunoperoxidase staining technique and by measurement of the serum alanine aminotransferase (ALT) activity. Three different antibody enzyme immunoassays (EIAs) were developed for assessment of the serologic response: 1) blocking assay, 2) IgM-specific capture assay, and 3) IgG-specific capture assay. An antigen-specific EIA was also developed for monitoring delta antigen in the serum. The results indicate that a superinfection with the DV produced a biphasic ALT response concomitant with the appearance of the delta antigen in biopsy materials. The persistence of these markers over the observation period (350 days) indicates the development of a persistent DV infection.

Full text

PDF
308

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonino F., Hoyer B., Shih J. W., Rizzetto M., Purcell R. H., Gerin J. L. Delta hepatitis agent: structural and antigenic properties of the delta-associated particle. Infect Immun. 1984 Mar;43(3):1000–1005. doi: 10.1128/iai.43.3.1000-1005.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradley D. W., Cook E. H., Maynard J. E., McCaustland K. A., Ebert J. W., Dolana G. H., Petzel R. A., Kantor R. J., Heilbrunn A., Fields H. A. Experimental infection of chimpanzees with antihemophilic (factor VIII) materials: recovery of virus-like particles associated with non-A, non-B hepatitis. J Med Virol. 1979;3(4):253–269. doi: 10.1002/jmv.1890030403. [DOI] [PubMed] [Google Scholar]
  3. Briantais M. J., Grangeot-Keros L., Pillot J. Specificity and sensitivity of the IgM capture immunoassay: studies of possible factors inducing false positive or false negative results. J Virol Methods. 1984 Aug;9(1):15–26. doi: 10.1016/0166-0934(84)90079-x. [DOI] [PubMed] [Google Scholar]
  4. Casali P., Bossus A., Carpentier N. A., Lambert P. H. Solid-phase enzyme immunoassay or radioimmunoassay for the detection of immune complexes based on their recognition by conglutinin: conglutinin-binding test. A comparative study with 125I-labelled Clq binding and Raji-cell RIA tests. Clin Exp Immunol. 1977 Aug;29(2):342–354. [PMC free article] [PubMed] [Google Scholar]
  5. Ishizaka T., Ishizaka K., Salmon S., Fudenberg H. Biologic activities of aggregated gamma-globulin. 8. Aggregated immunoglobulins of different classes. J Immunol. 1967 Jul;99(1):82–91. [PubMed] [Google Scholar]
  6. Maire M. A., Barnet M., Lambert P. H. Purification of bovine conglutinin using pepsin digestion. Mol Immunol. 1981 Feb;18(2):85–89. doi: 10.1016/0161-5890(81)90073-0. [DOI] [PubMed] [Google Scholar]
  7. McCarthy D., Goddard D. H., Pell B. K., Holborow E. J. Intrinsically stable IgG aggregates. J Immunol Methods. 1981;41(1):63–74. doi: 10.1016/0022-1759(81)90274-x. [DOI] [PubMed] [Google Scholar]
  8. Parry J. V. Diagnosis of hepatitis A infection: comparative specificity of IgM capture assays using antigens derived from tissue cultures and marmoset faeces. J Virol Methods. 1984 Aug;9(1):35–44. doi: 10.1016/0166-0934(84)90081-8. [DOI] [PubMed] [Google Scholar]
  9. Purcell R. H., Gerin J. L., Rizzetto M., Ponzetto A., Bonino F., London W. T. Experimental transmission of the delta agent to chimpanzees. Prog Clin Biol Res. 1983;143:79–89. [PubMed] [Google Scholar]
  10. Rizzetto M., Canese M. G., Aricò S., Crivelli O., Trepo C., Bonino F., Verme G. Immunofluorescence detection of new antigen-antibody system (delta/anti-delta) associated to hepatitis B virus in liver and in serum of HBsAg carriers. Gut. 1977 Dec;18(12):997–1003. doi: 10.1136/gut.18.12.997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rizzetto M., Canese M. G., Gerin J. L., London W. T., Sly D. L., Purcell R. H. Transmission of the hepatitis B virus-associated delta antigen to chimpanzees. J Infect Dis. 1980 May;141(5):590–602. doi: 10.1093/infdis/141.5.590. [DOI] [PubMed] [Google Scholar]
  12. Rizzetto M., Canese M. G., Purcell R. H., London W. T., Sly L. D., Gerin J. L. Experimental HBV and delta infections of chimpanzees: occurrence and significance of intrahepatic immune complexes of HBcAg and delta antigen. Hepatology. 1981 Nov-Dec;1(6):567–574. doi: 10.1002/hep.1840010602. [DOI] [PubMed] [Google Scholar]
  13. Rizzetto M., Hoyer B., Canese M. G., Shih J. W., Purcell R. H., Gerin J. L. delta Agent: association of delta antigen with hepatitis B surface antigen and RNA in serum of delta-infected chimpanzees. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6124–6128. doi: 10.1073/pnas.77.10.6124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shattock A. G., Morgan B. M. Sensitive enzyme immunoassay for the detection of delta antigen and anti-delta, using serum as the delta antigen source. J Med Virol. 1984;13(1):73–82. doi: 10.1002/jmv.1890130109. [DOI] [PubMed] [Google Scholar]
  15. Smedile A., Dentico P., Zanetti A., Sagnelli E., Nordenfelt E., Actis G. C., Rizzetto M. Infection with the delta agent in chronic HBsAg carriers. Gastroenterology. 1981 Dec;81(6):992–997. [PubMed] [Google Scholar]
  16. Smedile A., Lavarini C., Crivelli O., Raimondo G., Fassone M., Rizzetto M. Radioimmunoassay detection of IgM antibodies to the HBV-associated delta (delta) antigen:" clinical significance in delta infection. J Med Virol. 1982;9(2):131–138. doi: 10.1002/jmv.1890090208. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES