Abstract
Antibody responses to Four Corners hantavirus (FCV) infections in the deer mouse (Peromyscus maniculatus) were characterized by using FCV nucleocapsid protein (N), glycoprotein 1 (G1), and glycoprotein 2 (G2) recombinant polypeptides in Western immunoblot assays. Strong immunoglobulin G reactivities to FCV N were observed among FCV-infected wild P. maniculatus mice (n = 34) and in laboratory-infected P. maniculatus mice (n = 11). No immunoglobulin G antibody reactivities to FCV G1 or G2 linear determinants were detected. The strongest N responses were mapped to an amino-proximal segment between amino acids 17 and 59 (QLVTARQKLKDAERAVELDPDDVNKSTLQSRRAAVSALETKLG). FCV N antibodies cross-reacted with recombinant N proteins encoded by Puumala, Seoul, and Hantaan viruses.
Full Text
The Full Text of this article is available as a PDF (234.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Arthur R. R., Lofts R. S., Gomez J., Glass G. E., Leduc J. W., Childs J. E. Grouping of hantaviruses by small (S) genome segment polymerase chain reaction and amplification of viral RNA from wild-caught rats. Am J Trop Med Hyg. 1992 Aug;47(2):210–224. doi: 10.4269/ajtmh.1992.47.210. [DOI] [PubMed] [Google Scholar]
- Brummer-Korvenkontio M., Vaheri A., Hovi T., von Bonsdorff C. H., Vuorimies J., Manni T., Penttinen K., Oker-Blom N., Lähdevirta J. Nephropathia epidemica: detection of antigen in bank voles and serologic diagnosis of human infection. J Infect Dis. 1980 Feb;141(2):131–134. doi: 10.1093/infdis/141.2.131. [DOI] [PubMed] [Google Scholar]
- Childs J. E., Ksiazek T. G., Spiropoulou C. F., Krebs J. W., Morzunov S., Maupin G. O., Gage K. L., Rollin P. E., Sarisky J., Enscore R. E. Serologic and genetic identification of Peromyscus maniculatus as the primary rodent reservoir for a new hantavirus in the southwestern United States. J Infect Dis. 1994 Jun;169(6):1271–1280. doi: 10.1093/infdis/169.6.1271. [DOI] [PubMed] [Google Scholar]
- Chu Y. K., Rossi C., Leduc J. W., Lee H. W., Schmaljohn C. S., Dalrymple J. M. Serological relationships among viruses in the Hantavirus genus, family Bunyaviridae. Virology. 1994 Jan;198(1):196–204. doi: 10.1006/viro.1994.1022. [DOI] [PubMed] [Google Scholar]
- Duchin J. S., Koster F. T., Peters C. J., Simpson G. L., Tempest B., Zaki S. R., Ksiazek T. G., Rollin P. E., Nichol S., Umland E. T. Hantavirus pulmonary syndrome: a clinical description of 17 patients with a newly recognized disease. The Hantavirus Study Group. N Engl J Med. 1994 Apr 7;330(14):949–955. doi: 10.1056/NEJM199404073301401. [DOI] [PubMed] [Google Scholar]
- Elliott L. H., Kiley M. P., McCormick J. B. Hantaan virus: identification of virion proteins. J Gen Virol. 1984 Aug;65(Pt 8):1285–1293. doi: 10.1099/0022-1317-65-8-1285. [DOI] [PubMed] [Google Scholar]
- Hjelle B., Chavez-Giles F., Torrez-Martinez N., Yates T., Sarisky J., Webb J., Ascher M. Genetic identification of a novel hantavirus of the harvest mouse Reithrodontomys megalotis. J Virol. 1994 Oct;68(10):6751–6754. doi: 10.1128/jvi.68.10.6751-6754.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hjelle B., Jenison S., Torrez-Martinez N., Yamada T., Nolte K., Zumwalt R., MacInnes K., Myers G. A novel hantavirus associated with an outbreak of fatal respiratory disease in the southwestern United States: evolutionary relationships to known hantaviruses. J Virol. 1994 Feb;68(2):592–596. doi: 10.1128/jvi.68.2.592-596.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jenison S. A., Firzlaff J. M., Langenberg A., Galloway D. A. Identification of immunoreactive antigens of human papillomavirus type 6b by using Escherichia coli-expressed fusion proteins. J Virol. 1988 Jun;62(6):2115–2123. doi: 10.1128/jvi.62.6.2115-2123.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jenison S. A., Yu X. P., Valentine J. M., Galloway D. A. Characterization of human antibody-reactive epitopes encoded by human papillomavirus types 16 and 18. J Virol. 1991 Mar;65(3):1208–1218. doi: 10.1128/jvi.65.3.1208-1218.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jenison S., Yamada T., Morris C., Anderson B., Torrez-Martinez N., Keller N., Hjelle B. Characterization of human antibody responses to four corners hantavirus infections among patients with hantavirus pulmonary syndrome. J Virol. 1994 May;68(5):3000–3006. doi: 10.1128/jvi.68.5.3000-3006.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koerner T. J., Hill J. E., Myers A. M., Tzagoloff A. High-expression vectors with multiple cloning sites for construction of trpE fusion genes: pATH vectors. Methods Enzymol. 1991;194:477–490. doi: 10.1016/0076-6879(91)94036-c. [DOI] [PubMed] [Google Scholar]
- Lee H. W., Baek L. J., Johnson K. M. Isolation of Hantaan virus, the etiologic agent of Korean hemorrhagic fever, from wild urban rats. J Infect Dis. 1982 Nov;146(5):638–644. doi: 10.1093/infdis/146.5.638. [DOI] [PubMed] [Google Scholar]
- Lee H. W., Lee P. W., Johnson K. M. Isolation of the etiologic agent of Korean Hemorrhagic fever. J Infect Dis. 1978 Mar;137(3):298–308. doi: 10.1093/infdis/137.3.298. [DOI] [PubMed] [Google Scholar]
- Lundkvist A., Fatouros A., Niklasson B. Antigenic variation of European haemorrhagic fever with renal syndrome virus strains characterized using bank vole monoclonal antibodies. J Gen Virol. 1991 Sep;72(Pt 9):2097–2103. doi: 10.1099/0022-1317-72-9-2097. [DOI] [PubMed] [Google Scholar]
- Lundkvist A., Niklasson B. Bank vole monoclonal antibodies against Puumala virus envelope glycoproteins: identification of epitopes involved in neutralization. Arch Virol. 1992;126(1-4):93–105. doi: 10.1007/BF01309687. [DOI] [PubMed] [Google Scholar]
- Maina C. V., Riggs P. D., Grandea A. G., 3rd, Slatko B. E., Moran L. S., Tagliamonte J. A., McReynolds L. A., Guan C. D. An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. Gene. 1988 Dec 30;74(2):365–373. doi: 10.1016/0378-1119(88)90170-9. [DOI] [PubMed] [Google Scholar]
- Nichol S. T., Spiropoulou C. F., Morzunov S., Rollin P. E., Ksiazek T. G., Feldmann H., Sanchez A., Childs J., Zaki S., Peters C. J. Genetic identification of a hantavirus associated with an outbreak of acute respiratory illness. Science. 1993 Nov 5;262(5135):914–917. doi: 10.1126/science.8235615. [DOI] [PubMed] [Google Scholar]
- Ruo S. L., Sanchez A., Elliott L. H., Brammer L. S., McCormick J. B., Fisher-Hoch S. P. Monoclonal antibodies to three strains of hantaviruses: Hantaan, R22, and Puumala. Arch Virol. 1991;119(1-2):1–11. doi: 10.1007/BF01314318. [DOI] [PubMed] [Google Scholar]
- Schmaljohn C. S., Hasty S. E., Dalrymple J. M., LeDuc J. W., Lee H. W., von Bonsdorff C. H., Brummer-Korvenkontio M., Vaheri A., Tsai T. F., Regnery H. L. Antigenic and genetic properties of viruses linked to hemorrhagic fever with renal syndrome. Science. 1985 Mar 1;227(4690):1041–1044. doi: 10.1126/science.2858126. [DOI] [PubMed] [Google Scholar]
- Schmaljohn C. S., Jennings G. B., Hay J., Dalrymple J. M. Coding strategy of the S genome segment of Hantaan virus. Virology. 1986 Dec;155(2):633–643. doi: 10.1016/0042-6822(86)90223-0. [DOI] [PubMed] [Google Scholar]
- Schmaljohn C. S., Schmaljohn A. L., Dalrymple J. M. Hantaan virus M RNA: coding strategy, nucleotide sequence, and gene order. Virology. 1987 Mar;157(1):31–39. doi: 10.1016/0042-6822(87)90310-2. [DOI] [PubMed] [Google Scholar]
- Spiropoulou C. F., Morzunov S., Feldmann H., Sanchez A., Peters C. J., Nichol S. T. Genome structure and variability of a virus causing hantavirus pulmonary syndrome. Virology. 1994 May 1;200(2):715–723. doi: 10.1006/viro.1994.1235. [DOI] [PubMed] [Google Scholar]
- Vapalahti O., Kallio-Kokko H., Salonen E. M., Brummer-Korvenkontio M., Vaheri A. Cloning and sequencing of Puumala virus Sotkamo strain S and M RNA segments: evidence for strain variation in hantaviruses and expression of the nucleocapsid protein. J Gen Virol. 1992 Apr;73(Pt 4):829–838. doi: 10.1099/0022-1317-73-4-829. [DOI] [PubMed] [Google Scholar]
- Xiao S. Y., Leduc J. W., Chu Y. K., Schmaljohn C. S. Phylogenetic analyses of virus isolates in the genus Hantavirus, family Bunyaviridae. Virology. 1994 Jan;198(1):205–217. doi: 10.1006/viro.1994.1023. [DOI] [PubMed] [Google Scholar]
- Xiao S. Y., Spik K. W., Li D., Schmaljohn C. S. Nucleotide and deduced amino acid sequences of the M and S genome segments of two Puumala virus isolates from Russia. Virus Res. 1993 Oct;30(1):97–103. doi: 10.1016/0168-1702(93)90019-j. [DOI] [PubMed] [Google Scholar]
- di Guan C., Li P., Riggs P. D., Inouye H. Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. Gene. 1988 Jul 15;67(1):21–30. doi: 10.1016/0378-1119(88)90004-2. [DOI] [PubMed] [Google Scholar]