Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
letter
. 1986 Apr;123(1):9–15.

HTLV-III/LAV Viral Antigens in Lymph Nodes of Homosexual Men With Persistent Generalized Lymphadenopathy and AIDS

Klara Tenner-Racz, P Racz, Margarita Bofill, Anke Schulz-Meyer, M Dietrich, P Kern, J Weber, A J Pinching, Fulvia Veronese-Dimarzo, M Popovic, D Klatzmann, J C Gluckman, G Janossy
PMCID: PMC1888153  PMID: 3008562

Abstract

The presence of core antigens of retrovirus HTLV-III/LAV, referred to as “AIDS-related virus” (AV), has been sought in lymph node samples of patients with persistent generalized lymphadenopathy (PGL, 28 patients), prodromal AIDS (1 patient) and AIDS with Kaposi sarcoma (3 patients). In 30 patients the deposition of viral antigens, detected by monoclonal antibodies to HTLV-III and LAV, could be observed within the germinal centers (GCs) primarily within the extracellular network of immune complexes, and the two patients who were negative were atypical. No AV could be found in normal tonsil or in samples with follicular hyperplasia of unknown etiology (20 cases). These findings, taken together with the ultrastructural identification of typical retrovirus particles in all 9 PGL and 2 AIDS cases studied, indicates that the network of follicular dendritic (FD) cells is an important reservoir of AV virus antigen at this site. The persistence of this retrovirus inside the GCs helps explain how the follicular hyperplasia affecting FD cells and B blasts in PGL may in progressive cases be accompanied by destruction of FD cells and gradual development of T4+ lymphopenia. T4+ T cells may circulate through the GCs and become infected with AV there. In addition, the identification of retrovirus antigen in situ may be of diagnostic value.

Full text

PDF
9

Images in this article

11Figure 1
on p.11
12Figure 2
on p.12

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong J. A., Horne R. Follicular dendritic cells and virus-like particles in AIDS-related lymphadenopathy. Lancet. 1984 Aug 18;2(8399):370–372. doi: 10.1016/s0140-6736(84)90540-3. [DOI] [PubMed] [Google Scholar]
  2. Barré-Sinoussi F., Chermann J. C., Rey F., Nugeyre M. T., Chamaret S., Gruest J., Dauguet C., Axler-Blin C., Vézinet-Brun F., Rouzioux C. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science. 1983 May 20;220(4599):868–871. doi: 10.1126/science.6189183. [DOI] [PubMed] [Google Scholar]
  3. Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. doi: 10.1177/32.2.6198355. [DOI] [PubMed] [Google Scholar]
  4. Dalgleish A. G., Beverley P. C., Clapham P. R., Crawford D. H., Greaves M. F., Weiss R. A. The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature. 1984 Dec 20;312(5996):763–767. doi: 10.1038/312763a0. [DOI] [PubMed] [Google Scholar]
  5. Dorsett B., Cronin W., Chuma V., Ioachim H. L. Anti-lymphocyte antibodies in patients with the acquired immune deficiency syndrome. Am J Med. 1985 Apr;78(4):621–626. doi: 10.1016/0002-9343(85)90405-x. [DOI] [PubMed] [Google Scholar]
  6. Gallo R. C., Salahuddin S. Z., Popovic M., Shearer G. M., Kaplan M., Haynes B. F., Palker T. J., Redfield R., Oleske J., Safai B. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science. 1984 May 4;224(4648):500–503. doi: 10.1126/science.6200936. [DOI] [PubMed] [Google Scholar]
  7. Goodall A. H., Meek F. L., Waters J. A., Miescher G. C., Janossy G., Thomas H. C. A rapid one-step radiometric assay for hepatitis B surface antigen utilizing monoclonal antibodies. J Immunol Methods. 1982 Jul 30;52(2):167–174. doi: 10.1016/0022-1759(82)90042-4. [DOI] [PubMed] [Google Scholar]
  8. Griffiths P. D., Panjwani D. D., Stirk P. R., Ball M. G., Ganczakowski M., Blacklock H. A., Prentice H. G. Rapid diagnosis of cytomegalovirus infection in immunocompromised patients by detection of early antigen fluorescent foci. Lancet. 1984 Dec 1;2(8414):1242–1245. doi: 10.1016/s0140-6736(84)92797-1. [DOI] [PubMed] [Google Scholar]
  9. Haynes B. F., Robert-Guroff M., Metzgar R. S., Franchini G., Kalyanaraman V. S., Palker T. J., Gallo R. C. Monoclonal antibody against human T cell leukemia virus p19 defines a human thymic epithelial antigen acquired during ontogeny. J Exp Med. 1983 Mar 1;157(3):907–920. doi: 10.1084/jem.157.3.907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Humphrey J. H. Virus-like particles in AIDS-related lymphadenopathy. Lancet. 1984 Sep 15;2(8403):643–643. doi: 10.1016/s0140-6736(84)90636-6. [DOI] [PubMed] [Google Scholar]
  11. Janossy G., Pinching A. J., Bofill M., Weber J., McLaughlin J. E., Ornstein M., Ivory K., Harris J. R., Favrot M., Macdonald-Burns D. C. An immunohistological approach to persistent lymphadenopathy and its relevance to AIDS. Clin Exp Immunol. 1985 Feb;59(2):257–266. [PMC free article] [PubMed] [Google Scholar]
  12. Klatzmann D., Barré-Sinoussi F., Nugeyre M. T., Danquet C., Vilmer E., Griscelli C., Brun-Veziret F., Rouzioux C., Gluckman J. C., Chermann J. C. Selective tropism of lymphadenopathy associated virus (LAV) for helper-inducer T lymphocytes. Science. 1984 Jul 6;225(4657):59–63. doi: 10.1126/science.6328660. [DOI] [PubMed] [Google Scholar]
  13. Lane H. C., Masur H., Edgar L. C., Whalen G., Rook A. H., Fauci A. S. Abnormalities of B-cell activation and immunoregulation in patients with the acquired immunodeficiency syndrome. N Engl J Med. 1983 Aug 25;309(8):453–458. doi: 10.1056/NEJM198308253090803. [DOI] [PubMed] [Google Scholar]
  14. Mandel T. E., Phipps R. P., Abbot A., Tew J. G. The follicular dendritic cell: long term antigen retention during immunity. Immunol Rev. 1980;53:29–59. doi: 10.1111/j.1600-065x.1980.tb01039.x. [DOI] [PubMed] [Google Scholar]
  15. Mathur-Wagh U., Mildvan D. Prodromal syndromes in AIDS. Ann N Y Acad Sci. 1984;437:184–191. doi: 10.1111/j.1749-6632.1984.tb37135.x. [DOI] [PubMed] [Google Scholar]
  16. Montagnier L., Gruest J., Chamaret S., Dauguet C., Axler C., Guétard D., Nugeyre M. T., Barré-Sinoussi F., Chermann J. C., Brunet J. B. Adaptation of lymphadenopathy associated virus (LAV) to replication in EBV-transformed B lymphoblastoid cell lines. Science. 1984 Jul 6;225(4657):63–66. doi: 10.1126/science.6328661. [DOI] [PubMed] [Google Scholar]
  17. Parwaresch M. R., Radzun H. J., Hansmann M. L., Peters K. P. Monoclonal antibody Ki-M4 specifically recognizes human dendritic reticulum cells (follicular dendritic cells) and their possible precursor in blood. Blood. 1983 Sep;62(3):585–590. [PubMed] [Google Scholar]
  18. Pereira L., Hoffman M., Gallo D., Cremer N. Monoclonal antibodies to human cytomegalovirus: three surface membrane proteins with unique immunological and electrophoretic properties specify cross-reactive determinants. Infect Immun. 1982 Jun;36(3):924–932. doi: 10.1128/iai.36.3.924-932.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rácz P., Feller A. C., Tenner-Rácz K., Luther B., Lennert K., Dietrich M., Kern P. Human lymphocyte subpopulations in lymphadenopathies in homosexual men. Adv Exp Med Biol. 1985;186:1069–1076. doi: 10.1007/978-1-4613-2463-8_129. [DOI] [PubMed] [Google Scholar]
  20. Rácz P., Tenner-Rácz K., Kahl C., Feller A. C., Kern P., Dietrich M. Spectrum of morphologic changes of lymph nodes from patients with AIDS or AIDS-related complexes. Prog Allergy. 1986;37:81–181. doi: 10.1159/000318442. [DOI] [PubMed] [Google Scholar]
  21. Tenner-Rácz K., Rácz P., Dietrich M., Kern P. Altered follicular dendritic cells and virus-like particles in AIDS and AIDS-related lymphadenopathy. Lancet. 1985 Jan 12;1(8420):105–106. doi: 10.1016/s0140-6736(85)91994-4. [DOI] [PubMed] [Google Scholar]
  22. di Marzo Veronese F., Sarngadharan M. G., Rahman R., Markham P. D., Popovic M., Bodner A. J., Gallo R. C. Monoclonal antibodies specific for p24, the major core protein of human T-cell leukemia virus type III. Proc Natl Acad Sci U S A. 1985 Aug;82(15):5199–5202. doi: 10.1073/pnas.82.15.5199. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES