Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1986 Apr;123(1):161–165.

Minimal invasive carcinoma of the colon in rats.

C A Rubio, G Nylander, M Sveander, A Duvander, M L Alun
PMCID: PMC1888166  PMID: 3963149

Abstract

The careful histologic examination of the macroscopically normal colon in rats treated 8 months previously with a single dose of the colonotropic carcinogen dimethylhydrazine (DMH) demonstrated atypical colonic glands in normally occurring lymphoid mucosal patches. The atypical glands were characterized by the absence of cytoplasmic mucus, hyperchromatic cell nuclei, cell stratification, and increased number of mitotic figures. In 61.7% of the experimental animals, atypical colonic glands were found beyond the boundary of the muscularis mucosae, but in none of the controls. Thus, microinvasive adenocarcinoma of the colon seems to be a common finding in rats treated with a single dose of DMH. These adenocarcinomas remain, however, undetected at macroscopic examination. Measurements performed in consecutive lymphoid aggregates demonstrated that 50% of rats receiving DMH had thicker lymphoid aggregates than control animals. In spite of this, no tumor was recorded at naked eye examination in these areas. The macroscopic examination appears to be an unreliable method of ruling out malignancy in experimental rats treated with a single dose of the above-mentioned carcinogen.

Full text

PDF
161

Images in this article

162Figure 1
on p.162
162Figure 2
on p.162
163Figure 3
on p.163
163Figure 4
on p.163
164Figure 5
on p.164
164Figure 6
on p.164

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carachi R., Busuttil A., Joffe S. N., Blumgart L. H. Multiple polyps and colonic neoplasia induced in the rat. J Surg Res. 1980 Oct;29(4):363–370. doi: 10.1016/0022-4804(80)90070-0. [DOI] [PubMed] [Google Scholar]
  2. Deasy J. M., Steele G., Jr, Ross D. S., Lahey S. J., Wilson R. E., Madara J. Gut-associated lymphoid tissue and dimethylhydrazine-induced colorectal carcinoma in the Wistar/Furth rat. J Surg Oncol. 1983 Sep;24(1):36–40. doi: 10.1002/jso.2930240109. [DOI] [PubMed] [Google Scholar]
  3. Evans J. T., Shows T. B., Sproul E. E., Paolini N. S., Mittelman A., Hauschka T. S. Genetics of colon carcinogenesis in mice treated with 1,2-dimethylhydrazine. Cancer Res. 1977 Jan;37(1):134–136. [PubMed] [Google Scholar]
  4. Kikkawa N. Experimental studies of polypogenesis and carcinogenesis of the large intestine. Med J Osaka Univ. 1974 Mar;24(4):293–314. [PubMed] [Google Scholar]
  5. LaMont J. T., O'Gorman T. A. Experimental colon cancer. Gastroenterology. 1978 Dec;75(6):1157–1169. [PubMed] [Google Scholar]
  6. Maskens A. P., Dujardin-Loits R. M. Experimental adenomas and carcinomas of the large intestine behave as distinct entities: most carcinomas arise de novo in flat mucosa. Cancer. 1981 Jan 1;47(1):81–89. doi: 10.1002/1097-0142(19810101)47:1<81::aid-cncr2820470115>3.0.co;2-t. [DOI] [PubMed] [Google Scholar]
  7. Newberne P. M., Rogers A. E. Animal model of human disease. Adenocarcinoma of the colon. Am J Pathol. 1973 Sep;72(3):541–544. [PMC free article] [PubMed] [Google Scholar]
  8. Rubio C. A., Nylander G. Further studies on the carcinogenesis of the colon of the rat with special reference to the absence of intestinal contents. Cancer. 1981 Aug 15;48(4):951–953. doi: 10.1002/1097-0142(19810815)48:4<951::aid-cncr2820480416>3.0.co;2-u. [DOI] [PubMed] [Google Scholar]
  9. Rubio C. A., Nylander G., Santos M. Experimental colon cancer in the absence of intestinal contents in Sprague-Dawley rats. J Natl Cancer Inst. 1980 Mar;64(3):569–572. [PubMed] [Google Scholar]
  10. Rubio C. A., Nylander G. Surgical resection of the rat colon: effects on carcinogenesis by 1,2-dimethylhydrazine. J Natl Cancer Inst. 1982 May;68(5):813–815. [PubMed] [Google Scholar]
  11. Rubio C. A., Nylander G., Wallin B., Sveander M., Alun M. L., Duvander A. Carcinogenesis at colonic anastomotic sites. An animal study. Dis Colon Rectum. 1984 Jul;27(7):468–470. doi: 10.1007/BF02555543. [DOI] [PubMed] [Google Scholar]
  12. Rubio C. A., Nylander G., Wallin B., Sveander M., Alun M. L., Duvander A. Partial colon resection as a promotor of cancer growth in the rat. J Surg Oncol. 1984 Dec;27(4):236–238. doi: 10.1002/jso.2930270408. [DOI] [PubMed] [Google Scholar]
  13. Sunter J. P., Appleton D. R., Wright N. A., Watson A. J. Pathological features of the colonic tumours induced in rats by the administration of 1,2-dimethylhydrazine. Virchows Arch B Cell Pathol. 1978 Dec 21;29(3):211–223. doi: 10.1007/BF02899354. [DOI] [PubMed] [Google Scholar]
  14. Ward J. M. Morphogenesis of chemically induced neoplasms of the colon and small intestine in rats. Lab Invest. 1974 Apr;30(4):505–513. [PubMed] [Google Scholar]
  15. Ward J. M., Rice J. M., Roller P. P., Wenk M. L. Natural history of intestinal neoplasms induced in rats by a single injection of methyl(acetoxymethyl)nitrosamine. Cancer Res. 1977 Sep;37(9):3046–3052. [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES