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. 1986 Jul;124(1):53–58.

In vivo induction of neutrophilia, lymphopenia, and diminution of neutrophil adhesion by stable analogs of prostaglandins E1, E2, and F2 alpha.

T R Ulich, E B Dakay, J H Williams, R X Ni
PMCID: PMC1888171  PMID: 3728647

Abstract

Stable analogs of prostaglandins E1, E2, and F2 alpha (M-PGE1, DM-PGE2, and M-PGF2 alpha) were found to induce marked changes in circulating white blood cell subsets in Brown-Norway rats after subcutaneous injection. Dose-response studies demonstrated that 1000 micrograms/kg of each prostaglandin induced a maximum neutrophilia in the range of 40-70% of the total white blood cell count (normal, 5-20%) and that as little as 5 micrograms/kg of M-PGE1 induced a significant neutrophilia (P less than 0.05). Kinetic studies demonstrated that the maximum neutrophilia occurred 4-6 hours after injection of each prostaglandin and was not accompanied by the release of morphologically immature neutrophil forms from the bone marrow. Splenectomy slightly diminished the average neutrophilia at 2 hours but not at 4-6 hours after injection, which suggests that release of neutrophils from the spleen partially contributed to the early neutrophilia. Adherence experiments employing whole heparinized blood from rats given prostaglandins 6 hours prior to sacrifice demonstrated that neutrophils exposed to prostaglandins in vivo have diminished adherence to nylon wool columns, which suggests that diminished adherence of the marginated neutrophil pool may contribute to the neutrophilia. The prostaglandin-induced neutrophilia was accompanied not by a significant change in total numbers of circulating white blood cells, but, rather, by a significant decrease in circulating mononuclear white blood cells, including T-helper, T-suppressor, and B cells. The combination of neutrophilia with lymphopenia has classically been attributed to the release of adrenal hormones and suggests 1) that prostaglandins may directly or indirectly cause the release of adrenal hormones, or 2) that adrenal hormones may mediate their effects on circulating white blood cell subsets via prostaglandins, or 3) that prostaglandins activate intracellular messenger systems that are also activated by adrenal hormones.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barclay A. N. The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 1981 Apr;42(4):593–600. [PMC free article] [PubMed] [Google Scholar]
  2. Brideau R. J., Carter P. B., McMaster W. R., Mason D. W., Williams A. F. Two subsets of rat T lymphocytes defined with monoclonal antibodies. Eur J Immunol. 1980 Aug;10(8):609–615. doi: 10.1002/eji.1830100807. [DOI] [PubMed] [Google Scholar]
  3. Crum E. D. The subset of T lymphocytes mediating delayed-type hypersensitivity in the rat. Cell Immunol. 1983 Apr 15;77(2):385–394. doi: 10.1016/0008-8749(83)90039-4. [DOI] [PubMed] [Google Scholar]
  4. Duarte A. J., Carpenter C. B., Strom T. B. Expression of T cell differentiation antigens and Ia on rat cytotoxic T lymphocytes. J Immunol. 1982 Feb;128(2):580–584. [PubMed] [Google Scholar]
  5. Fantone J. C., Kunkel R. G., Kinnes D. A. Potentiation of alpha-naphthyl thiourea-induced lung injury by prostaglandin E1 and platelet depletion. Lab Invest. 1984 Jun;50(6):703–710. [PubMed] [Google Scholar]
  6. Fantone J. C., Kunkel S. L., Ward P. A. Suppression of human polymorphonuclear function after intravenous infusion of prostaglandin E1. Prostaglandins Med. 1981 Aug;7(2):195–198. doi: 10.1016/0161-4630(81)90062-8. [DOI] [PubMed] [Google Scholar]
  7. Fantone J. C., Marasco W. A., Elgas L. J., Ward P. A. Anti-inflammatory effects of prostaglandin E1: in vivo modulation of the formyl peptide chemotactic receptor on the rat neutrophil. J Immunol. 1983 Apr;130(4):1495–1497. [PubMed] [Google Scholar]
  8. Fantone J. C., Marasco W. A., Elgas L. J., Ward P. A. Stimulus specificity of prostaglandin inhibition of rabbit polymorphonuclear leukocyte lysosomal enzyme release and superoxide anion production. Am J Pathol. 1984 Apr;115(1):9–16. [PMC free article] [PubMed] [Google Scholar]
  9. GORDON A. S. Some aspects of hormonal influences upon the leukocytes. Ann N Y Acad Sci. 1955 Mar 24;59(5):907–927. doi: 10.1111/j.1749-6632.1955.tb45990.x. [DOI] [PubMed] [Google Scholar]
  10. Issekutz A. C. Effect of vasoactive agents on polymorphonuclear leukocyte emigration in vivo. Lab Invest. 1981 Sep;45(3):234–240. [PubMed] [Google Scholar]
  11. Kunkel S. L., Fantone J. C., Ward P. A., Zurier R. B. Modulation of inflammatory reactions by prostaglandins. Prog Lipid Res. 1981;20:633–640. doi: 10.1016/0163-7827(81)90118-1. [DOI] [PubMed] [Google Scholar]
  12. Kunkel S. L., Thrall R. S., Kunkel R. G., McCormick J. R., Ward P. A., Zurier R. B. Suppression of immune complex vasculitis in rats by prostaglandin. J Clin Invest. 1979 Nov;64(5):1525–1529. doi: 10.1172/JCI109611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kurosawa M., Nemoto T., Aoki H., Ike A., Abe O., Sunaga Y., Kobayashi S. Prostaglandin-induced eosinopenia in splenectomized rats. J Allergy Clin Immunol. 1978 Jul;62(1):33–36. doi: 10.1016/0091-6749(78)90070-2. [DOI] [PubMed] [Google Scholar]
  14. Lomnitzer R., Rabson A. R., Koornhof H. J. The effects of cyclic AMP on leucocyte inhibitory factor (LIF) production and on the inhibition of leucocyte migration. Clin Exp Immunol. 1976 Apr;24(1):42–48. [PMC free article] [PubMed] [Google Scholar]
  15. MacGregor R. R., Spagnuolo P. J., Lentnek A. L. Inhibition of granulocyte adherence by ethanol, prednisone, and aspirin, measured with an assay system. N Engl J Med. 1974 Sep 26;291(13):642–646. doi: 10.1056/NEJM197409262911302. [DOI] [PubMed] [Google Scholar]
  16. Marsden K. A., Rao P. S., Cavanagh D., Spaziani E. The effect of prostaglandin D2 (PGD2) on circulating eosinophils. Prostaglandins Leukot Med. 1984 Sep;15(3):387–397. doi: 10.1016/0262-1746(84)90137-9. [DOI] [PubMed] [Google Scholar]
  17. McMaster W. R., Williams A. F. Identification of Ia glycoproteins in rat thymus and purification from rat spleen. Eur J Immunol. 1979 Jun;9(6):426–433. doi: 10.1002/eji.1830090603. [DOI] [PubMed] [Google Scholar]
  18. Till G., Kownatzki E., Seitz M., Gemsa D. Chemokinetic and chemotactic activity of various prostaglandins for neutrophil granulocytes. Clin Immunol Immunopathol. 1979 Jan;12(1):111–118. doi: 10.1016/0090-1229(79)90116-8. [DOI] [PubMed] [Google Scholar]
  19. Ulich T. R., Bannister K. M., Wilson C. B. Tubulointerstitial nephritis induced in the brown Norway rat with chaotropically solubilized bovine tubular basement membrane: the model and the humoral and cellular responses. Clin Immunol Immunopathol. 1985 Aug;36(2):187–200. doi: 10.1016/0090-1229(85)90120-5. [DOI] [PubMed] [Google Scholar]
  20. Ulich T. R., Dakay E. B., Williams J. H., Ni R. X., Cairo M. S. (15S)-15-methyl prostaglandin E1-induced neutrophilia in Brown-Norway rats. Prostaglandins Leukot Med. 1985 Nov;20(2):169–173. doi: 10.1016/0262-1746(85)90007-1. [DOI] [PubMed] [Google Scholar]
  21. White R. A., Mason D. W., Williams A. F., Galfre G., Milstein C. T-lymphocyte heterogeneity in the rat: separation of functional subpopulations using a monoclonal antibody. J Exp Med. 1978 Sep 1;148(3):664–673. doi: 10.1084/jem.148.3.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Williams A. F., Galfrè G., Milstein C. Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell. 1977 Nov;12(3):663–673. doi: 10.1016/0092-8674(77)90266-5. [DOI] [PubMed] [Google Scholar]

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