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. 1995 Mar;69(3):1971–1979. doi: 10.1128/jvi.69.3.1971-1979.1995

Constitutive and inducible kappa B binding activities in the cytosol of v-Rel-transformed lymphoid cells.

J Hodgson 1, P J Enrietto 1
PMCID: PMC188819  PMID: 7853544

Abstract

Constitutive and inducible kapp B binding activities associated with v-Rel and c-Rel in the cytosol of v-Rel-transformed cells have been identified. These activities were resolved by electrophoretic mobility shift assay and chromatographic techniques into a high-molecular-weight protein-DNA complex designated complex I containing v- and c-Rel and lower-molecular-weight complexes II, III and IV which contained only v-Rel and which were stimulated by nucleotides, low pH, and detergent. These experiments suggest that interaction of v-Rel and c-Rel decreases the DNA-binding activity of each.

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Selected References

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