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. 1986 Jun;123(3):425–431.

Follicular large cell lymphoma. An immunophenotype study.

C F Garcia, R A Warnke, L M Weiss
PMCID: PMC1888276  PMID: 3521302

Abstract

The authors investigated 17 cases of follicular large cell lymphoma using monoclonal antibodies applied to frozen sections. The neoplastic cells in 11 cases (65%) showed evidence of immunoglobulin expression similar to the reported percentage of immunoglobulin expressing diffuse large cell lymphomas and lower than seen in low grade follicular lymphomas. All cases showed expression of the B lineage markers T015, B1, and 4G7, and HLA-DR. CALLA was present in all but 1 case, similar to that reported for follicular lymphomas, and much higher than reported for diffuse large cell lymphoma. Approximately one-half of the cases showed weak expression of Tac, 5 cases expressed B2 (C3d), and 3 cases expressed T05 (C3b). Variable expression was seen for the Ki-67 antigen. A CR4/23+, B2+, T05+ dendritic population was identified in all cases. An interfollicular host T-cell infiltrate was noted, mainly phenotypic helper cells. This study demonstrates that follicular large cell lymphoma has immunologic similarities to both diffuse large cell lymphoma and the low grade follicular lymphomas.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Doggett R. S., Wood G. S., Horning S., Levy R., Dorfman R. F., Bindl J., Warnke R. A. The immunologic characterization of 95 nodal and extranodal diffuse large cell lymphomas in 89 patients. Am J Pathol. 1984 May;115(2):245–252. [PMC free article] [PubMed] [Google Scholar]
  2. Dvoretsky P., Wood G. S., Levy R., Warnke R. A. T-lymphocyte subsets in follicular lymphomas compared with those in non-neoplastic lymph nodes and tonsils. Hum Pathol. 1982 Jul;13(7):618–625. doi: 10.1016/s0046-8177(82)80003-8. [DOI] [PubMed] [Google Scholar]
  3. Freedman A. S., Boyd A. W., Anderson K. C., Fisher D. C., Pinkus G. S., Schlossman S. F., Nadler L. M. Immunologic heterogeneity of diffuse large cell lymphoma. Blood. 1985 Mar;65(3):630–637. [PubMed] [Google Scholar]
  4. Gerdes J., Naiem M., Mason D. Y., Stein H. Human complement (C3b) receptors defined by a mouse monoclonal antibody. Immunology. 1982 Apr;45(4):645–653. [PMC free article] [PubMed] [Google Scholar]
  5. Gerdes J., Schwab U., Lemke H., Stein H. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 15;31(1):13–20. doi: 10.1002/ijc.2910310104. [DOI] [PubMed] [Google Scholar]
  6. Gerdes J., Stein H. Complement (C3) receptors on dendritic reticulum cells of normal and malignant lymphoid tissue. Clin Exp Immunol. 1982 May;48(2):348–352. [PMC free article] [PubMed] [Google Scholar]
  7. Grogan T. M., Hicks M. J., Jolley C. S., Rangel C. S., Jones S. E. Identification of two major B cell forms of nodular mixed lymphoma. Lab Invest. 1984 Nov;51(5):504–514. [PubMed] [Google Scholar]
  8. Halper J. P., Knowles D. M., 2nd, Wang C. Y. Ia antigen expression by human malignant lymphomas: correlation with conventional lymphoid markers. Blood. 1980 Mar;55(3):373–382. [PubMed] [Google Scholar]
  9. Harris N. L., Bhan A. K. Distribution of T-cell subsets in follicular and diffuse lymphomas of B-cell type. Am J Pathol. 1983 Nov;113(2):172–180. [PMC free article] [PubMed] [Google Scholar]
  10. Harris N. L., Nadler L. M., Bhan A. K. Immunohistologic characterization of two malignant lymphomas of germinal center type (centroblastic/centrocytic and centrocytic) with monoclonal antibodies. Follicular and diffuse lymphomas of small-cleaved-cell type are related but distinct entities. Am J Pathol. 1984 Nov;117(2):262–272. [PMC free article] [PubMed] [Google Scholar]
  11. Hsu S. M., Yang K., Jaffe E. S. Hairy cell leukemia: a B cell neoplasm with a unique antigenic phenotype. Am J Clin Pathol. 1983 Oct;80(4):421–428. doi: 10.1093/ajcp/80.4.421. [DOI] [PubMed] [Google Scholar]
  12. Hsu S. M., Yang K., Jaffe E. S. Phenotypic expression of Hodgkin's and Reed-Sternberg cells in Hodgkin's disease. Am J Pathol. 1985 Feb;118(2):209–217. [PMC free article] [PubMed] [Google Scholar]
  13. Iida K., Nadler L., Nussenzweig V. Identification of the membrane receptor for the complement fragment C3d by means of a monoclonal antibody. J Exp Med. 1983 Oct 1;158(4):1021–1033. doi: 10.1084/jem.158.4.1021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jaffe E. S., Shevach E. M., Frank M. M., Berard C. W., Green I. Nodular lymphoma--evidence for origin from follicular B lymphocytes. N Engl J Med. 1974 Apr 11;290(15):813–819. doi: 10.1056/NEJM197404112901501. [DOI] [PubMed] [Google Scholar]
  15. Lennert K., Kikuchi M., Sato E., Suchi T., Stansfeld A. G., Feller A. C., Hansmann M. L., Müller-Hermelink H. K., Gödde-Salz E. HTLV-positive and -negative T-cell lymphomas. Morphological and immunohistochemical differences between European and HTLV-positive Japanese T-cell lymphomas. Int J Cancer. 1985 Jan 15;35(1):65–72. doi: 10.1002/ijc.2910350111. [DOI] [PubMed] [Google Scholar]
  16. Levy R., Warnke R., Dorfman R. F., Haimovich J. The monoclonality of human B-cell lymphomas. J Exp Med. 1977 Apr 1;145(4):1014–1028. doi: 10.1084/jem.145.4.1014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nadler L. M., Stashenko P., Hardy R., van Agthoven A., Terhorst C., Schlossman S. F. Characterization of a human B cell-specific antigen (B2) distinct from B1. J Immunol. 1981 May;126(5):1941–1947. [PubMed] [Google Scholar]
  18. Naiem M., Gerdes J., Abdulaziz Z., Stein H., Mason D. Y. Production of a monoclonal antibody reactive with human dendritic reticulum cells and its use in the immunohistological analysis of lymphoid tissue. J Clin Pathol. 1983 Feb;36(2):167–175. doi: 10.1136/jcp.36.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stashenko P., Nadler L. M., Hardy R., Schlossman S. F. Expression of cell surface markers after human B lymphocyte activation. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3848–3852. doi: 10.1073/pnas.78.6.3848. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Swerdlow S. H., Murray L. J., Habeshaw J. A., Stansfeld A. G. B- and T-cell subsets in follicular centroblastic/centrocytic (cleaved follicular center cell) lymphoma: an immunohistologic analysis of 26 lymph nodes and three spleens. Hum Pathol. 1985 Apr;16(4):339–352. doi: 10.1016/s0046-8177(85)80228-8. [DOI] [PubMed] [Google Scholar]
  21. Uchiyama T., Broder S., Waldmann T. A. A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. I. Production of anti-Tac monoclonal antibody and distribution of Tac (+) cells. J Immunol. 1981 Apr;126(4):1393–1397. [PubMed] [Google Scholar]
  22. Warnke R., Levy R. Immunopathology of follicular lymphomas. A model of B-lymphocyte homing. N Engl J Med. 1978 Mar 2;298(9):481–486. doi: 10.1056/NEJM197803022980903. [DOI] [PubMed] [Google Scholar]
  23. Wood G. S., Turner R. R., Shiurba R. A., Eng L., Warnke R. A. Human dendritic cells and macrophages. In situ immunophenotypic definition of subsets that exhibit specific morphologic and microenvironmental characteristics. Am J Pathol. 1985 Apr;119(1):73–82. [PMC free article] [PubMed] [Google Scholar]
  24. Wood G. S., Warnke R. A. The immunologic phenotyping of bone marrow biopsies and aspirates: frozen section techniques. Blood. 1982 May;59(5):913–922. [PubMed] [Google Scholar]

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