Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1986 Sep;124(3):373–376.

DNA aneuploidy in follicular adenomas of the thyroid gland.

H Joensuu, P Klemi, E Eerola
PMCID: PMC1888353  PMID: 3766698

Abstract

The nuclear DNA content of 67 follicular adenomas and 7 adenomatous goiters was determined by flow cytometry. The DNA analysis was done with the use of paraffin-embedded tissue, but in 14 cases of follicular adenoma fresh material obtained by fine needle aspiration biopsy was also available. An abnormal DNA stemline was found in 27% (18/67) of the adenomas and in 1 (14%) of the adenomatous goiters. Four (29%) of the 14 adenomas analyzed from fresh samples were aneuploid. In none of the 12 aneuploid or 40 diploid adenomas followed for more than 5 years (mean, 7.2 years) did distant metastases develop after surgical removal of the adenoma. The paraffin blocks of cases with DNA aneuploidy were sectioned subserially at 1-mm intervals, but none of these tumors showed invasion into the capsule or blood vessels. It is concluded that DNA aneuploidy is common in histologically benign follicular adenomas of the thyroid, and that DNA aneuploidy does not appear to be associated with adverse prognosis in follicular adenomas if the tumor is surgically removed.

Full text

PDF
373

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barlogie B., Raber M. N., Schumann J., Johnson T. S., Drewinko B., Swartzendruber D. E., Göhde W., Andreeff M., Freireich E. J. Flow cytometry in clinical cancer research. Cancer Res. 1983 Sep;43(9):3982–3997. [PubMed] [Google Scholar]
  2. Bengtsson A., Malmaeus J., Grimelius L., Johansson H., Pontén J., Rastad J., Akerström G. Measurement of nuclear DNA content in thyroid diagnosis. World J Surg. 1984 Aug;8(4):481–486. doi: 10.1007/BF01654918. [DOI] [PubMed] [Google Scholar]
  3. Friedlander M. L., Hedley D. W., Taylor I. W. Clinical and biological significance of aneuploidy in human tumours. J Clin Pathol. 1984 Sep;37(9):961–974. doi: 10.1136/jcp.37.9.961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Greenebaum E., Koss L. G., Elequin F., Silver C. E. The diagnostic value of flow cytometric DNA measurements in follicular tumors of the thyroid gland. Cancer. 1985 Oct 15;56(8):2011–2018. doi: 10.1002/1097-0142(19851015)56:8<2011::aid-cncr2820560821>3.0.co;2-v. [DOI] [PubMed] [Google Scholar]
  5. Hammarberg C., Slezak P., Tribukait B. Early detection of malignancy in ulcerative colitis. A flow-cytometric DNA study. Cancer. 1984 Jan 15;53(2):291–295. doi: 10.1002/1097-0142(19840115)53:2<291::aid-cncr2820530218>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
  6. Hedley D. W., Friedlander M. L., Taylor I. W., Rugg C. A., Musgrove E. A. Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem. 1983 Nov;31(11):1333–1335. doi: 10.1177/31.11.6619538. [DOI] [PubMed] [Google Scholar]
  7. Johannessen J. V., Sobrinho-Simões M., Lindmo T., Tangen K. O. The diagnostic value of flow cytometric DNA measurements in selected disorders of the human thyroid. Am J Clin Pathol. 1982 Jan;77(1):20–25. doi: 10.1093/ajcp/77.1.20. [DOI] [PubMed] [Google Scholar]
  8. Kraemer B. B., Srigley J. R., Batsakis J. G., Silva E. G., Goepfert H. DNA flow cytometry of thyroid neoplasms. Arch Otolaryngol. 1985 Jan;111(1):34–38. doi: 10.1001/archotol.1985.00800030068008. [DOI] [PubMed] [Google Scholar]
  9. Lukács G. L., Balázs G., Zs-Nagy I. Cytofluorimetric measurements on the DNA contents of tumor cells in human thyroid gland. J Cancer Res Clin Oncol. 1979;95(3):265–271. doi: 10.1007/BF00410647. [DOI] [PubMed] [Google Scholar]
  10. Rabenhorst G. The diagnostic value of the DNA content of fine needle biopsies of the thyroid gland. Virchows Arch B Cell Pathol. 1974;16(4):379–383. doi: 10.1007/BF02894090. [DOI] [PubMed] [Google Scholar]
  11. Stephenson R. A., Gay H., Fair W. R., Melamed M. R. Effect of section thickness on quality of flow cytometric DNA content determinations in paraffin-embedded tissues. Cytometry. 1986 Jan;7(1):41–44. doi: 10.1002/cyto.990070106. [DOI] [PubMed] [Google Scholar]
  12. Vindeløv L. L., Christensen I. J., Nissen N. I. A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry. 1983 Mar;3(5):323–327. doi: 10.1002/cyto.990030503. [DOI] [PubMed] [Google Scholar]
  13. van den Ingh H. F., Griffioen G., Cornelisse C. J. Flow cytometric detection of aneuploidy in colorectal adenomas. Cancer Res. 1985 Jul;45(7):3392–3397. [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES