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. 1986 Sep;124(3):427–435.

Mast cells in human keloid, small intestine, and lung by an immunoperoxidase technique using a murine monoclonal antibody against tryptase.

S S Craig, G DeBlois, L B Schwartz
PMCID: PMC1888358  PMID: 3532813

Abstract

A murine monoclonal antibody (G5) against human lung mast cell tryptase was used for selective staining of human mast cells by an indirect immunoperoxidase method. Human tissues (keloid, small bowel, lung) were fixed in either Carnoy's fluid or neutral buffered formalin. In all three tissues the number and location of G5-stained mast cells corresponded closely with metachromatic toluidine blue-stained mast cells, although the immunospecific technique appeared to be more sensitive. In lung the average concentration of G5-positive mast cells after Carnoy's fixation was 15,695/cu mm of subepithelial tissue in bronchi and bronchioles and 26,580/cu mm of alveolar wall, in small bowel was 20,958/cu mm of mucosa and 8576/cu mm of submucosa, and in keloid was 3068/cu mm. Formalin fixation significantly reduced concentrations of G5-positive mast cells in all tissues except keloid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrett K. E., Metcalfe D. D. Mast cell heterogeneity: evidence and implications. J Clin Immunol. 1984 Jul;4(4):253–261. doi: 10.1007/BF00915292. [DOI] [PubMed] [Google Scholar]
  2. Befus A. D., Johnston N., Bienenstock J. Nippostrongylus brasiliensis: mast cells and histamine levels in tissues of infected and normal rats. Exp Parasitol. 1979 Aug;48(1):1–8. doi: 10.1016/0014-4894(79)90048-1. [DOI] [PubMed] [Google Scholar]
  3. Befus D., Goodacre R., Dyck N., Bienenstock J. Mast cell heterogeneity in man. I. Histologic studies of the intestine. Int Arch Allergy Appl Immunol. 1985;76(3):232–236. doi: 10.1159/000233697. [DOI] [PubMed] [Google Scholar]
  4. Chiu H., Lagunoff D. Histochemical comparison of vertebrate mast cells. Histochem J. 1972 Mar;4(2):135–144. doi: 10.1007/BF01004972. [DOI] [PubMed] [Google Scholar]
  5. Combs J. W., Lagunoff D., Benditt E. P. Differentiation and proliferation of embryonic mast cells of the rat. J Cell Biol. 1965 Jun;25(3):577–592. doi: 10.1083/jcb.25.3.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Enerbäck L. Mast cells in rat gastrointestinal mucosa. I. Effects of fixation. Acta Pathol Microbiol Scand. 1966;66(3):289–302. doi: 10.1111/apm.1966.66.3.289. [DOI] [PubMed] [Google Scholar]
  7. Fox B., Bull T. B., Guz A. Mast cells in the human alveolar wall: an electronmicroscopic study. J Clin Pathol. 1981 Dec;34(12):1333–1342. doi: 10.1136/jcp.34.12.1333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GLENNER G. G., COHEN L. A. Histochemical demonstration of a species-specific trypsin-like enzyme in mast cells. Nature. 1960 Mar 19;185:846–847. doi: 10.1038/185846a0. [DOI] [PubMed] [Google Scholar]
  9. Haig D. M., McKee T. A., Jarrett E. E., Woodbury R., Miller H. R. Generation of mucosal mast cells is stimulated in vitro by factors derived from T cells of helminth-infected rats. Nature. 1982 Nov 11;300(5888):188–190. doi: 10.1038/300188a0. [DOI] [PubMed] [Google Scholar]
  10. Hall W. B. Staining mast cells in human gingiva. Arch Oral Biol. 1966 Dec;11(12):1325–1336. doi: 10.1016/0003-9969(66)90023-9. [DOI] [PubMed] [Google Scholar]
  11. Kido H., Fukusen N., Katunuma N. Chymotrypsin- and trypsin-type serine proteases in rat mast cells: properties and functions. Arch Biochem Biophys. 1985 Jun;239(2):436–443. doi: 10.1016/0003-9861(85)90709-x. [DOI] [PubMed] [Google Scholar]
  12. Mayrhofer G. The nature of the thymus dependency of mucosal mast cells. I. An adaptive secondary response to challenge with Nippostrongylus brasiliensis. Cell Immunol. 1979 Oct;47(2):304–311. doi: 10.1016/0008-8749(79)90340-x. [DOI] [PubMed] [Google Scholar]
  13. Ruitenberg E. J., Elgersma A., Kruizinga W. Intestinal mast cells and globule leucocytes: role of the thymus on their presence and proliferation during a Trichinella spiralis infection in the rat. Int Arch Allergy Appl Immunol. 1979;60(3):302–309. doi: 10.1159/000232355. [DOI] [PubMed] [Google Scholar]
  14. Ruitenberg E. J., Gustowska L., Elgersma A., Ruitenberg H. M. Effect of fixation on the light microscopical visualization of mast cells in the mucosa and connective tissue of the human duodenum. Int Arch Allergy Appl Immunol. 1982;67(3):233–238. doi: 10.1159/000233024. [DOI] [PubMed] [Google Scholar]
  15. SALVATO G. Mast cells in bronchial connective tissue of man: their modifications in asthma and after treatment with the histamine liberator 48/80. Int Arch Allergy Appl Immunol. 1961;18:348–358. doi: 10.1159/000229186. [DOI] [PubMed] [Google Scholar]
  16. Schechter N. M., Fräki J. E., Geesin J. C., Lazarus G. S. Human skin chymotryptic proteinase. Isolation and relation to cathepsin g and rat mast cell proteinase I. J Biol Chem. 1983 Mar 10;258(5):2973–2978. [PubMed] [Google Scholar]
  17. Schwartz L. B., Foley J. V., Austen K. F., Soter N. A., Shepard R., Murphy G. F. Localization of tryptase to human cutaneous mast cells and keratinocytes by immunofluorescence and immunoperoxidase cytochemistry with monoclonal antitryptase antibody. J Allergy Clin Immunol. 1985 Aug;76(2 Pt 1):182–188. doi: 10.1016/0091-6749(85)90699-2. [DOI] [PubMed] [Google Scholar]
  18. Schwartz L. B., Lewis R. A., Austen K. F. Tryptase from human pulmonary mast cells. Purification and characterization. J Biol Chem. 1981 Nov 25;256(22):11939–11943. [PubMed] [Google Scholar]
  19. Schwartz L. B. Monoclonal antibodies against human mast cell tryptase demonstrate shared antigenic sites on subunits of tryptase and selective localization of the enzyme to mast cells. J Immunol. 1985 Jan;134(1):526–531. [PubMed] [Google Scholar]
  20. Strobel S., Miller H. R., Ferguson A. Human intestinal mucosal mast cells: evaluation of fixation and staining techniques. J Clin Pathol. 1981 Aug;34(8):851–858. doi: 10.1136/jcp.34.8.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tharp M. D., Seelig L. L., Jr, Tigelaar R. E., Bergstresser P. R. Conjugated avidin binds to mast cell granules. J Histochem Cytochem. 1985 Jan;33(1):27–32. doi: 10.1177/33.1.2578142. [DOI] [PubMed] [Google Scholar]
  22. Wenzel S., Irani A. M., Sanders J. M., Bradford T. R., Schwartz L. B. Immunoassay of tryptase from human mast cells. J Immunol Methods. 1986 Jan 22;86(1):139–142. doi: 10.1016/0022-1759(86)90277-2. [DOI] [PubMed] [Google Scholar]
  23. Woodbury R. G., Neurath H. Structure, specificity and localization of the serine proteases of connective tissue. FEBS Lett. 1980 Jun 2;114(2):189–196. doi: 10.1016/0014-5793(80)81112-4. [DOI] [PubMed] [Google Scholar]
  24. Zachrisson B. U. Mast cells of the human gingiva. 3. Histochemical demonstration of immature mast cells in chronically inflamed tissue. J Periodontal Res. 1968;3(2):136–145. doi: 10.1111/j.1600-0765.1968.tb01914.x. [DOI] [PubMed] [Google Scholar]

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