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. 1986 Oct;125(1):208–217.

The in vitro growth, heterotransplantation, and differentiation of a human rhabdomyosarcoma cell line.

A J Garvin, W S Stanley, D D Bennett, J L Sullivan, D A Sens
PMCID: PMC1888450  PMID: 3777136

Abstract

A human rhabdomyosarcoma (RMS) cell line was established from a case of childhood small cell sarcoma, which in vivo showed no evidence of differentiation, but which demonstrated myogenic differentiation in tissue culture. In a serum-free culture medium, the tumor cells demonstrated continuous growth without ultrastructural or biochemical evidence of differentiation. Heterotransplanted RMS cells gave rise to tumors in nude mice which also showed no myogenic differentiation. However, RMS cells grown in the presence of either retinoic acid (5 microns), phorbol ester (1 nM), prostaglandin E1 (10 ng/ml), or 2% fetal calf serum gave rise to myotubes with a biochemical shift in the creatine kinase isoenzyme pattern from the embryonic to the mature skeletal muscle form. The karyotype of the RMS cells revealed a translocation of Chromosomes 2 and 13, which may represent a nonrandom aberration unique to this morphologic subtype. In addition, the RMS cells gave evidence for gene amplification in the form of double minute chromosomes. This human RMS cell line provides a valuable in vitro system for study of myogenesis and factors which induce differentiation.

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Selected References

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  1. Altmannsberger M., Osborn M., Treuner J., Hölscher A., Weber K., Shauer A. Diagnosis of human childhood rhabdomyosarcoma of antibodies to desmin, the structural protein of muscle specific intermediate filaments. Virchows Arch B Cell Pathol Incl Mol Pathol. 1982;39(2):203–215. doi: 10.1007/BF02892848. [DOI] [PubMed] [Google Scholar]
  2. Becher R., Wake N., Gibas Z., Ochi H., Sandberg A. A. Chromosome changes in soft tissue sarcomas. J Natl Cancer Inst. 1984 Apr;72(4):823–831. [PubMed] [Google Scholar]
  3. Dexter D. L. N,N-Dimethylformamide-induced morphological differentiation and reduction of tumorigenicity in cultured mouse rhabdomyosarcoma cells. Cancer Res. 1977 Sep;37(9):3136–3140. [PubMed] [Google Scholar]
  4. Foxall C. D., Emery A. E. Changes in creatine kinase and its isoenzymes in human fetal muscle during development. J Neurol Sci. 1975 Apr;24(4):483–492. doi: 10.1016/0022-510x(75)90172-0. [DOI] [PubMed] [Google Scholar]
  5. Freshney R. I. Induction of differentiation in neoplastic cells. Anticancer Res. 1985 Jan-Feb;5(1):111–130. [PubMed] [Google Scholar]
  6. Garvin A. J., Drew S. W., Hintz D. S., Sens D. A. Tissue culture as a diagnostic technique for soft-tissue sarcoma of childhood. Arch Pathol Lab Med. 1984 Apr;108(4):308–310. [PubMed] [Google Scholar]
  7. Garvin A. J., Surrette F., Hintz D. S., Rudisill M. T., Sens M. A., Sens D. A. The in vitro growth and characterization of the skeletal muscle component of Wilms' tumor. Am J Pathol. 1985 Nov;121(2):298–310. [PMC free article] [PubMed] [Google Scholar]
  8. Molenaar W. M., Oosterhuis J. W., Kamps W. A. Cytologic "differentiation" in childhood rhabdomyosarcomas following polychemotherapy. Hum Pathol. 1984 Oct;15(10):973–979. doi: 10.1016/s0046-8177(84)80127-6. [DOI] [PubMed] [Google Scholar]
  9. Mukai K., Rosai J., Hallaway B. E. Localization of myoglobin in normal and neoplastic human skeletal muscle cells using an immunoperoxidase method. Am J Surg Pathol. 1979 Aug;3(4):373–376. doi: 10.1097/00000478-197908000-00008. [DOI] [PubMed] [Google Scholar]
  10. Mukai M., Iri H., Torikata C., Kageyama K., Morikawa Y., Shimizu K. Immunoperoxidase demonstration of a new muscle protein (Z-protein) in myogenic tumors as a diagnostic aid. Am J Pathol. 1984 Jan;114(1):164–170. [PMC free article] [PubMed] [Google Scholar]
  11. Nelson-Rees W. A., Flandermeyer R. R., Hawthorne P. K. Distinctive banded marker chromosomes of human tumor cell lines. Int J Cancer. 1975 Jul 15;16(1):74–82. doi: 10.1002/ijc.2910160109. [DOI] [PubMed] [Google Scholar]
  12. Sarnat H. B., deMello D. E., Siddiqui S. Y. Diagnostic value of histochemistry in embryonal rhabdomyosarcoma. Am J Surg Pathol. 1979 Apr;3(2):177–183. doi: 10.1097/00000478-197904000-00010. [DOI] [PubMed] [Google Scholar]
  13. Seeger R. C., Brodeur G. M., Sather H., Dalton A., Siegel S. E., Wong K. Y., Hammond D. Association of multiple copies of the N-myc oncogene with rapid progression of neuroblastomas. N Engl J Med. 1985 Oct 31;313(18):1111–1116. doi: 10.1056/NEJM198510313131802. [DOI] [PubMed] [Google Scholar]
  14. Seidal T., Mark J., Hagmar B., Angervall L. Alveolar rhabdomyosarcoma: a cytogenetic and correlated cytological and histological study. Acta Pathol Microbiol Immunol Scand A. 1982 Sep;90(5):345–354. doi: 10.1111/j.1699-0463.1982.tb00105_90a.x. [DOI] [PubMed] [Google Scholar]
  15. Szasz G., Gruber W., Bernt E. Creatine kinase in serum: 1. Determination of optimum reaction conditions. Clin Chem. 1976 May;22(5):650–656. [PubMed] [Google Scholar]
  16. Trent J., Casper J., Meltzer P., Thompson F., Fogh J. Nonrandom chromosome alterations in rhabdomyosarcoma. Cancer Genet Cytogenet. 1985 Apr 1;16(3):189–197. doi: 10.1016/0165-4608(85)90045-7. [DOI] [PubMed] [Google Scholar]
  17. Tsokos M., Howard R., Costa J. Immunohistochemical study of alveolar and embryonal rhabdomyosarcoma. Lab Invest. 1983 Feb;48(2):148–155. [PubMed] [Google Scholar]
  18. Turc-Carel C., Lizard-Nacol S., Justrabo E., Favrot M., Philip T., Tabone E. Consistent chromosomal translocation in alveolar rhabdomyosarcoma. Cancer Genet Cytogenet. 1986 Jan 15;19(3-4):361–362. doi: 10.1016/0165-4608(86)90069-5. [DOI] [PubMed] [Google Scholar]
  19. Wold L. E., Li C. Y., Homburger H. A. Localization of the B and M polypeptide subunits of creatine kinase in normal and neoplastic human tissues by an immunoperoxidase technic. Am J Clin Pathol. 1981 Mar;75(3):327–332. doi: 10.1093/ajcp/75.3.327. [DOI] [PubMed] [Google Scholar]
  20. de Jong A. S., van Kessel-van Vark M., Albus-Lutter C. E., van Raamsdonk W., Voûte P. A. Skeletal muscle actin as tumor marker in the diagnosis of rhabdomyosarcoma in childhood. Am J Surg Pathol. 1985 Jul;9(7):467–474. doi: 10.1097/00000478-198507000-00001. [DOI] [PubMed] [Google Scholar]

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